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Enhanced vascularization in hybrid PCL/gelatin fibrous scaffolds with sustained release of VEGF.

Wang K, Chen X, Pan Y, Cui Y, Zhou X, Kong D, Zhao Q - Biomed Res Int (2015)

Bottom Line: In vitro release test confirms the sustained releasing profile of VEGF, and stable release was observed over a time period of 25 days.In vitro cell assay indicates that VEGF release significantly promoted the proliferation of endothelial cells.More importantly, in vivo subcutaneous implantation reflects that vascularization has been effectively enhanced in the PCL/gelatin scaffolds compared with the PCL counterpart due to the sustained release of VEGF.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, China.

ABSTRACT
Creating a long-lasting and functional vasculature represents one of the most fundamental challenges in tissue engineering. VEGF has been widely accepted as a potent angiogenic factor involved in the early stages of blood vessel formation. In this study, fibrous scaffolds that consist of PCL and gelatin fibers were fabricated. The gelatin fibers were further functionalized by heparin immobilization, which provides binding sites for VEGF and thus enables the sustained release of VEGF. In vitro release test confirms the sustained releasing profile of VEGF, and stable release was observed over a time period of 25 days. In vitro cell assay indicates that VEGF release significantly promoted the proliferation of endothelial cells. More importantly, in vivo subcutaneous implantation reflects that vascularization has been effectively enhanced in the PCL/gelatin scaffolds compared with the PCL counterpart due to the sustained release of VEGF. Therefore, the heparinized PCL/gelatin scaffolds developed in this study may be a promising candidate for regeneration of complex tissues with sufficient vascularization.

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Related in: MedlinePlus

Mechanical properties of PCL and PCL/Gel scaffolds with or without heparization.
© Copyright Policy - open-access
Related In: Results  -  Collection


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fig3: Mechanical properties of PCL and PCL/Gel scaffolds with or without heparization.

Mentions: The mechanical properties were evaluated by tensile test (Figure 3 and Table 3). Neat PCL is a ductile polymer with high degree of elongation, that is, 614.90 ± 13.34%. The incorporation of gelatin component fails to alter the flexible mechanical characteristic; only tensile strength was slightly lowered because the weight ratio of the gelatin in the scaffold is relatively low. Heparization strengthens the PCL/Gel scaffolds effectively with evidently enhanced tensile strength and Young's modulus. At the same time, the elongation at break was adversely decreased due to the formation of 3D molecular network during the crosslinking. Similar trends in mechanical properties have been reported before [21]. However in this case the elongation for heparinized PCL/Gel is still higher than 100%, which is higher than the normal displacement of the artery during the physiological dilation and constriction, and satisfies the requirement of artificial vascular grafts. In a word, the mechanical properties of the scaffolds could be readily tuned within a wide range to satisfy the requirement of vascular grafts.


Enhanced vascularization in hybrid PCL/gelatin fibrous scaffolds with sustained release of VEGF.

Wang K, Chen X, Pan Y, Cui Y, Zhou X, Kong D, Zhao Q - Biomed Res Int (2015)

Mechanical properties of PCL and PCL/Gel scaffolds with or without heparization.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4390103&req=5

fig3: Mechanical properties of PCL and PCL/Gel scaffolds with or without heparization.
Mentions: The mechanical properties were evaluated by tensile test (Figure 3 and Table 3). Neat PCL is a ductile polymer with high degree of elongation, that is, 614.90 ± 13.34%. The incorporation of gelatin component fails to alter the flexible mechanical characteristic; only tensile strength was slightly lowered because the weight ratio of the gelatin in the scaffold is relatively low. Heparization strengthens the PCL/Gel scaffolds effectively with evidently enhanced tensile strength and Young's modulus. At the same time, the elongation at break was adversely decreased due to the formation of 3D molecular network during the crosslinking. Similar trends in mechanical properties have been reported before [21]. However in this case the elongation for heparinized PCL/Gel is still higher than 100%, which is higher than the normal displacement of the artery during the physiological dilation and constriction, and satisfies the requirement of artificial vascular grafts. In a word, the mechanical properties of the scaffolds could be readily tuned within a wide range to satisfy the requirement of vascular grafts.

Bottom Line: In vitro release test confirms the sustained releasing profile of VEGF, and stable release was observed over a time period of 25 days.In vitro cell assay indicates that VEGF release significantly promoted the proliferation of endothelial cells.More importantly, in vivo subcutaneous implantation reflects that vascularization has been effectively enhanced in the PCL/gelatin scaffolds compared with the PCL counterpart due to the sustained release of VEGF.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, China.

ABSTRACT
Creating a long-lasting and functional vasculature represents one of the most fundamental challenges in tissue engineering. VEGF has been widely accepted as a potent angiogenic factor involved in the early stages of blood vessel formation. In this study, fibrous scaffolds that consist of PCL and gelatin fibers were fabricated. The gelatin fibers were further functionalized by heparin immobilization, which provides binding sites for VEGF and thus enables the sustained release of VEGF. In vitro release test confirms the sustained releasing profile of VEGF, and stable release was observed over a time period of 25 days. In vitro cell assay indicates that VEGF release significantly promoted the proliferation of endothelial cells. More importantly, in vivo subcutaneous implantation reflects that vascularization has been effectively enhanced in the PCL/gelatin scaffolds compared with the PCL counterpart due to the sustained release of VEGF. Therefore, the heparinized PCL/gelatin scaffolds developed in this study may be a promising candidate for regeneration of complex tissues with sufficient vascularization.

Show MeSH
Related in: MedlinePlus