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Enhanced vascularization in hybrid PCL/gelatin fibrous scaffolds with sustained release of VEGF.

Wang K, Chen X, Pan Y, Cui Y, Zhou X, Kong D, Zhao Q - Biomed Res Int (2015)

Bottom Line: In vitro release test confirms the sustained releasing profile of VEGF, and stable release was observed over a time period of 25 days.In vitro cell assay indicates that VEGF release significantly promoted the proliferation of endothelial cells.More importantly, in vivo subcutaneous implantation reflects that vascularization has been effectively enhanced in the PCL/gelatin scaffolds compared with the PCL counterpart due to the sustained release of VEGF.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, China.

ABSTRACT
Creating a long-lasting and functional vasculature represents one of the most fundamental challenges in tissue engineering. VEGF has been widely accepted as a potent angiogenic factor involved in the early stages of blood vessel formation. In this study, fibrous scaffolds that consist of PCL and gelatin fibers were fabricated. The gelatin fibers were further functionalized by heparin immobilization, which provides binding sites for VEGF and thus enables the sustained release of VEGF. In vitro release test confirms the sustained releasing profile of VEGF, and stable release was observed over a time period of 25 days. In vitro cell assay indicates that VEGF release significantly promoted the proliferation of endothelial cells. More importantly, in vivo subcutaneous implantation reflects that vascularization has been effectively enhanced in the PCL/gelatin scaffolds compared with the PCL counterpart due to the sustained release of VEGF. Therefore, the heparinized PCL/gelatin scaffolds developed in this study may be a promising candidate for regeneration of complex tissues with sufficient vascularization.

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Related in: MedlinePlus

Structure characterization of electrospun PCL/Gel scaffolds. SEM images of PCL/Gel-1 (a), Hep-PCL/Gel-1 (b), and Hep-PCL/Gel-1 degraded in PBS for 14 days (c) and PCL/Gel-2 (d), Hep-PCL/Gel-2 (e), and Hep-PCL/Gel-2 degraded in PBS for 14 days (f) (scale bar = 20 μm). Fluorescent images of PCL/Gel-1 with red PCL fibers labled with DiI (g), and green gelatin fibers labled with DiO (h), as well as the merge one (i) (scale bar = 20 μm).
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fig1: Structure characterization of electrospun PCL/Gel scaffolds. SEM images of PCL/Gel-1 (a), Hep-PCL/Gel-1 (b), and Hep-PCL/Gel-1 degraded in PBS for 14 days (c) and PCL/Gel-2 (d), Hep-PCL/Gel-2 (e), and Hep-PCL/Gel-2 degraded in PBS for 14 days (f) (scale bar = 20 μm). Fluorescent images of PCL/Gel-1 with red PCL fibers labled with DiI (g), and green gelatin fibers labled with DiO (h), as well as the merge one (i) (scale bar = 20 μm).

Mentions: SEM images show two types of fibers with contrast in fiber size (Figures 1(a) and 1(d)). The fibers of large diameter are attributed to PCL, while those of small diameter correspond to gelatin, by comparing with the SEM images of neat PCL and gelatin [24]. The pore size of scaffolds was also determined based on the SEM images (Table 2).


Enhanced vascularization in hybrid PCL/gelatin fibrous scaffolds with sustained release of VEGF.

Wang K, Chen X, Pan Y, Cui Y, Zhou X, Kong D, Zhao Q - Biomed Res Int (2015)

Structure characterization of electrospun PCL/Gel scaffolds. SEM images of PCL/Gel-1 (a), Hep-PCL/Gel-1 (b), and Hep-PCL/Gel-1 degraded in PBS for 14 days (c) and PCL/Gel-2 (d), Hep-PCL/Gel-2 (e), and Hep-PCL/Gel-2 degraded in PBS for 14 days (f) (scale bar = 20 μm). Fluorescent images of PCL/Gel-1 with red PCL fibers labled with DiI (g), and green gelatin fibers labled with DiO (h), as well as the merge one (i) (scale bar = 20 μm).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4390103&req=5

fig1: Structure characterization of electrospun PCL/Gel scaffolds. SEM images of PCL/Gel-1 (a), Hep-PCL/Gel-1 (b), and Hep-PCL/Gel-1 degraded in PBS for 14 days (c) and PCL/Gel-2 (d), Hep-PCL/Gel-2 (e), and Hep-PCL/Gel-2 degraded in PBS for 14 days (f) (scale bar = 20 μm). Fluorescent images of PCL/Gel-1 with red PCL fibers labled with DiI (g), and green gelatin fibers labled with DiO (h), as well as the merge one (i) (scale bar = 20 μm).
Mentions: SEM images show two types of fibers with contrast in fiber size (Figures 1(a) and 1(d)). The fibers of large diameter are attributed to PCL, while those of small diameter correspond to gelatin, by comparing with the SEM images of neat PCL and gelatin [24]. The pore size of scaffolds was also determined based on the SEM images (Table 2).

Bottom Line: In vitro release test confirms the sustained releasing profile of VEGF, and stable release was observed over a time period of 25 days.In vitro cell assay indicates that VEGF release significantly promoted the proliferation of endothelial cells.More importantly, in vivo subcutaneous implantation reflects that vascularization has been effectively enhanced in the PCL/gelatin scaffolds compared with the PCL counterpart due to the sustained release of VEGF.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, China.

ABSTRACT
Creating a long-lasting and functional vasculature represents one of the most fundamental challenges in tissue engineering. VEGF has been widely accepted as a potent angiogenic factor involved in the early stages of blood vessel formation. In this study, fibrous scaffolds that consist of PCL and gelatin fibers were fabricated. The gelatin fibers were further functionalized by heparin immobilization, which provides binding sites for VEGF and thus enables the sustained release of VEGF. In vitro release test confirms the sustained releasing profile of VEGF, and stable release was observed over a time period of 25 days. In vitro cell assay indicates that VEGF release significantly promoted the proliferation of endothelial cells. More importantly, in vivo subcutaneous implantation reflects that vascularization has been effectively enhanced in the PCL/gelatin scaffolds compared with the PCL counterpart due to the sustained release of VEGF. Therefore, the heparinized PCL/gelatin scaffolds developed in this study may be a promising candidate for regeneration of complex tissues with sufficient vascularization.

Show MeSH
Related in: MedlinePlus