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Combined ginger extract & Gelam honey modulate Ras/ERK and PI3K/AKT pathway genes in colon cancer HT29 cells.

Tahir AA, Sani NF, Murad NA, Makpol S, Ngah WZ, Yusof YA - Nutr J (2015)

Bottom Line: The cells were divided into 4 groups: the first group represents HT29 cells without treatment, the second and third groups were cells treated singly with either ginger or Gelam honey, respectively, and the last group represents cells treated with ginger and Gelam honey combined.Cell death in response to the combined ginger and Gelam honey treatment was associated with the stimulation of early apoptosis (upregulation of caspase 9 and IκB genes) accompanied by downregulation of the KRAS, ERK, AKT, Bcl-xL, NFkB (p65) genes in a synergistic manner.In conclusion, the combination of ginger and Gelam honey may be an effective chemopreventive and therapeutic strategy for inducing the death of colon cancer cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, Faculty of Medicine, Universiti Kebangsaan Malaysia, Jalan Yaacob Latif, Bandar Tun Razak, Cheras, 56000, Kuala Lumpur, Malaysia. analbaik@gmail.com.

ABSTRACT

Background: The interconnected Ras/ERK and PI3K/AKT pathways play a central role in colorectal tumorigenesis, and they are targets for elucidating mechanisms involved in attempts to induce colon cancer cell death. Both ginger (Zingiber officinale) and honey have been shown to exhibit anti-tumor and anti-inflammation properties against many types of cancer, including colorectal cancer. However, there are currently no reports showing the combined effect of these two dietary compounds in cancer growth inhibition. The aim of this study was to evaluate the synergistic effect of crude ginger extract and Gelam honey in combination as potential cancer chemopreventive agents against the colorectal cancer cell line HT29.

Methods: The cells were divided into 4 groups: the first group represents HT29 cells without treatment, the second and third groups were cells treated singly with either ginger or Gelam honey, respectively, and the last group represents cells treated with ginger and Gelam honey combined.

Results: The results of MTS assay showed that the IC50 of ginger and Gelam honey alone were 5.2 mg/ml and 80 mg/ml, respectively, whereas the IC50 of the combination treatment was 3 mg/ml of ginger plus 27 mg/ml of Gelam honey with a combination index of < 1, suggesting synergism. Cell death in response to the combined ginger and Gelam honey treatment was associated with the stimulation of early apoptosis (upregulation of caspase 9 and IκB genes) accompanied by downregulation of the KRAS, ERK, AKT, Bcl-xL, NFkB (p65) genes in a synergistic manner.

Conclusions: In conclusion, the combination of ginger and Gelam honey may be an effective chemopreventive and therapeutic strategy for inducing the death of colon cancer cells.

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Related in: MedlinePlus

Effects of ginger and Gelam honey alone on viability of HT29 cells. Viability of HT29 cells with treatment of Ginger (A) and Gelam honey (B) was determined by MTS assay after 24 hour treatment with increasing concentrations of ginger or Gelam honey. Data are presented as the mean ± SEM from three independent experiments. *(p < 0.05) significant compared to without treatment.
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Fig1: Effects of ginger and Gelam honey alone on viability of HT29 cells. Viability of HT29 cells with treatment of Ginger (A) and Gelam honey (B) was determined by MTS assay after 24 hour treatment with increasing concentrations of ginger or Gelam honey. Data are presented as the mean ± SEM from three independent experiments. *(p < 0.05) significant compared to without treatment.

Mentions: Figure 1A and B show the IC50 of ginger extract and Gelam honey to be 5.2 mg/ml and 80 mg/ml, respectively. The viability of HT29 cells did not show any changes when treated at low concentrations with ginger (2 and 4 mg/ml) or Gelam honey (20, 40 and 60 mg/ml). Whereas at high concentration, both ginger and Gelam honey decreased the viability of HT29 cells significantly (p<0.05) compared to untreated cells.Figure 1


Combined ginger extract & Gelam honey modulate Ras/ERK and PI3K/AKT pathway genes in colon cancer HT29 cells.

Tahir AA, Sani NF, Murad NA, Makpol S, Ngah WZ, Yusof YA - Nutr J (2015)

Effects of ginger and Gelam honey alone on viability of HT29 cells. Viability of HT29 cells with treatment of Ginger (A) and Gelam honey (B) was determined by MTS assay after 24 hour treatment with increasing concentrations of ginger or Gelam honey. Data are presented as the mean ± SEM from three independent experiments. *(p < 0.05) significant compared to without treatment.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4390091&req=5

Fig1: Effects of ginger and Gelam honey alone on viability of HT29 cells. Viability of HT29 cells with treatment of Ginger (A) and Gelam honey (B) was determined by MTS assay after 24 hour treatment with increasing concentrations of ginger or Gelam honey. Data are presented as the mean ± SEM from three independent experiments. *(p < 0.05) significant compared to without treatment.
Mentions: Figure 1A and B show the IC50 of ginger extract and Gelam honey to be 5.2 mg/ml and 80 mg/ml, respectively. The viability of HT29 cells did not show any changes when treated at low concentrations with ginger (2 and 4 mg/ml) or Gelam honey (20, 40 and 60 mg/ml). Whereas at high concentration, both ginger and Gelam honey decreased the viability of HT29 cells significantly (p<0.05) compared to untreated cells.Figure 1

Bottom Line: The cells were divided into 4 groups: the first group represents HT29 cells without treatment, the second and third groups were cells treated singly with either ginger or Gelam honey, respectively, and the last group represents cells treated with ginger and Gelam honey combined.Cell death in response to the combined ginger and Gelam honey treatment was associated with the stimulation of early apoptosis (upregulation of caspase 9 and IκB genes) accompanied by downregulation of the KRAS, ERK, AKT, Bcl-xL, NFkB (p65) genes in a synergistic manner.In conclusion, the combination of ginger and Gelam honey may be an effective chemopreventive and therapeutic strategy for inducing the death of colon cancer cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, Faculty of Medicine, Universiti Kebangsaan Malaysia, Jalan Yaacob Latif, Bandar Tun Razak, Cheras, 56000, Kuala Lumpur, Malaysia. analbaik@gmail.com.

ABSTRACT

Background: The interconnected Ras/ERK and PI3K/AKT pathways play a central role in colorectal tumorigenesis, and they are targets for elucidating mechanisms involved in attempts to induce colon cancer cell death. Both ginger (Zingiber officinale) and honey have been shown to exhibit anti-tumor and anti-inflammation properties against many types of cancer, including colorectal cancer. However, there are currently no reports showing the combined effect of these two dietary compounds in cancer growth inhibition. The aim of this study was to evaluate the synergistic effect of crude ginger extract and Gelam honey in combination as potential cancer chemopreventive agents against the colorectal cancer cell line HT29.

Methods: The cells were divided into 4 groups: the first group represents HT29 cells without treatment, the second and third groups were cells treated singly with either ginger or Gelam honey, respectively, and the last group represents cells treated with ginger and Gelam honey combined.

Results: The results of MTS assay showed that the IC50 of ginger and Gelam honey alone were 5.2 mg/ml and 80 mg/ml, respectively, whereas the IC50 of the combination treatment was 3 mg/ml of ginger plus 27 mg/ml of Gelam honey with a combination index of < 1, suggesting synergism. Cell death in response to the combined ginger and Gelam honey treatment was associated with the stimulation of early apoptosis (upregulation of caspase 9 and IκB genes) accompanied by downregulation of the KRAS, ERK, AKT, Bcl-xL, NFkB (p65) genes in a synergistic manner.

Conclusions: In conclusion, the combination of ginger and Gelam honey may be an effective chemopreventive and therapeutic strategy for inducing the death of colon cancer cells.

Show MeSH
Related in: MedlinePlus