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Antimicrobial residual effects of irrigation regimens with maleic acid in infected root canals.

Ferrer-Luque CM, González-Castillo S, Ruiz-Linares M, Arias-Moliz MT, Rodríguez-Archilla A, Baca P - J Biol Res (Thessalon) (2015)

Bottom Line: The association of MA with CHX and CHX + CTR as final irrigating solutions showed the best results, 5 and 4 samples out of 12, respectively, and without differences between them (p = 0.928), followed by 7% MA + 0.2% CTR with 7 out of 12.The 7% MA (11/12) group showed significant differences with respect to the groups in which MA was combined with CHX (p < 0.005) and CHX + CTR (p < 0.005).Final irrigating solutions of 7% MA combined with 2% CHX or 2% CHX + 0.2% CTR were found to effectively improve antimicrobial root canal disinfection.

View Article: PubMed Central - PubMed

Affiliation: Department of Dental Pathology and Therapeutics, School of Dentistry, University of Granada, Campus de Cartuja, Colegio Máximo s/n, Granada, Spain.

ABSTRACT

Background: The success of endodontic treatment depends largely on the control of microorganisms present in infected root canals. The aim of this study was to determine the residual antimicrobial activity of several final irrigation protocols with 7% maleic acid (MA) alone and combined with chlorhexidine (CHX), cetrimide (CTR) or both, in root canals infected with Enterococcus faecalis. Biofilms of E. faecalis were grown in uniradicular roots for 4 weeks. A total of 72 specimens were divided into 5 experimental groups according to the final irrigation regime used: Group 1: 2.5% NaOCl; Group 2: 7% MA; Group 3: 7% MA + 0.2% CTR; Group 4: 7% MA + 2% CHX; Group 5: 7% MA + 0.2% CTR + 2% CHX; and

Control group: 0.9% saline solution. Twelve roots (2/group) that were instrumented and not infected served as negative or sterility controls. The proportion of ungrown samples over 60 days was evaluated using non-parametric Kaplan-Meier survival analysis. Differences among groups were tested using the log-rank test (p< 0.05).

Results: The association of MA with CHX and CHX + CTR as final irrigating solutions showed the best results, 5 and 4 samples out of 12, respectively, and without differences between them (p = 0.928), followed by 7% MA + 0.2% CTR with 7 out of 12. The 7% MA (11/12) group showed significant differences with respect to the groups in which MA was combined with CHX (p < 0.005) and CHX + CTR (p < 0.005).

Conclusion: Final irrigating solutions of 7% MA combined with 2% CHX or 2% CHX + 0.2% CTR were found to effectively improve antimicrobial root canal disinfection.

No MeSH data available.


Related in: MedlinePlus

Kaplan-Meier survival probabilities at 60 days (probability of no growth) for experimental groups. Cum survival: percentage of samples that did not show E. faecalis growth at a given time. For clarity: ++, 7% MA + 0.2% CTR. At day 30, there was growth of E. faecalis in 7 out of 12 samples, meaning that 41.67% survived without growing.
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Fig1: Kaplan-Meier survival probabilities at 60 days (probability of no growth) for experimental groups. Cum survival: percentage of samples that did not show E. faecalis growth at a given time. For clarity: ++, 7% MA + 0.2% CTR. At day 30, there was growth of E. faecalis in 7 out of 12 samples, meaning that 41.67% survived without growing.

Mentions: At 60 days, all samples in the 2.5% NaOCl group showed regrowth, exhibiting statistically significant differences with respect to all the other study groups. The 7% MA group, with regrowth of 91.66% of samples, showed significant differences with respect to the groups in which MA was combined with CHX (41.66%) and CHX + CTR (33.33%), but not when combined with only CTR (58.33%). The association of MA with CHX and with CHX + CTR showed the best results, with a very low number of regrown samples at 60 days (respectively, 5 and 4 samples out of 12). The results of Kaplan-Meier survival analysis, (excluding 0.9% SS group), are indicated in Figure 1 and its footnote.Figure 1


Antimicrobial residual effects of irrigation regimens with maleic acid in infected root canals.

Ferrer-Luque CM, González-Castillo S, Ruiz-Linares M, Arias-Moliz MT, Rodríguez-Archilla A, Baca P - J Biol Res (Thessalon) (2015)

Kaplan-Meier survival probabilities at 60 days (probability of no growth) for experimental groups. Cum survival: percentage of samples that did not show E. faecalis growth at a given time. For clarity: ++, 7% MA + 0.2% CTR. At day 30, there was growth of E. faecalis in 7 out of 12 samples, meaning that 41.67% survived without growing.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4390086&req=5

Fig1: Kaplan-Meier survival probabilities at 60 days (probability of no growth) for experimental groups. Cum survival: percentage of samples that did not show E. faecalis growth at a given time. For clarity: ++, 7% MA + 0.2% CTR. At day 30, there was growth of E. faecalis in 7 out of 12 samples, meaning that 41.67% survived without growing.
Mentions: At 60 days, all samples in the 2.5% NaOCl group showed regrowth, exhibiting statistically significant differences with respect to all the other study groups. The 7% MA group, with regrowth of 91.66% of samples, showed significant differences with respect to the groups in which MA was combined with CHX (41.66%) and CHX + CTR (33.33%), but not when combined with only CTR (58.33%). The association of MA with CHX and with CHX + CTR showed the best results, with a very low number of regrown samples at 60 days (respectively, 5 and 4 samples out of 12). The results of Kaplan-Meier survival analysis, (excluding 0.9% SS group), are indicated in Figure 1 and its footnote.Figure 1

Bottom Line: The association of MA with CHX and CHX + CTR as final irrigating solutions showed the best results, 5 and 4 samples out of 12, respectively, and without differences between them (p = 0.928), followed by 7% MA + 0.2% CTR with 7 out of 12.The 7% MA (11/12) group showed significant differences with respect to the groups in which MA was combined with CHX (p < 0.005) and CHX + CTR (p < 0.005).Final irrigating solutions of 7% MA combined with 2% CHX or 2% CHX + 0.2% CTR were found to effectively improve antimicrobial root canal disinfection.

View Article: PubMed Central - PubMed

Affiliation: Department of Dental Pathology and Therapeutics, School of Dentistry, University of Granada, Campus de Cartuja, Colegio Máximo s/n, Granada, Spain.

ABSTRACT

Background: The success of endodontic treatment depends largely on the control of microorganisms present in infected root canals. The aim of this study was to determine the residual antimicrobial activity of several final irrigation protocols with 7% maleic acid (MA) alone and combined with chlorhexidine (CHX), cetrimide (CTR) or both, in root canals infected with Enterococcus faecalis. Biofilms of E. faecalis were grown in uniradicular roots for 4 weeks. A total of 72 specimens were divided into 5 experimental groups according to the final irrigation regime used: Group 1: 2.5% NaOCl; Group 2: 7% MA; Group 3: 7% MA + 0.2% CTR; Group 4: 7% MA + 2% CHX; Group 5: 7% MA + 0.2% CTR + 2% CHX; and

Control group: 0.9% saline solution. Twelve roots (2/group) that were instrumented and not infected served as negative or sterility controls. The proportion of ungrown samples over 60 days was evaluated using non-parametric Kaplan-Meier survival analysis. Differences among groups were tested using the log-rank test (p< 0.05).

Results: The association of MA with CHX and CHX + CTR as final irrigating solutions showed the best results, 5 and 4 samples out of 12, respectively, and without differences between them (p = 0.928), followed by 7% MA + 0.2% CTR with 7 out of 12. The 7% MA (11/12) group showed significant differences with respect to the groups in which MA was combined with CHX (p < 0.005) and CHX + CTR (p < 0.005).

Conclusion: Final irrigating solutions of 7% MA combined with 2% CHX or 2% CHX + 0.2% CTR were found to effectively improve antimicrobial root canal disinfection.

No MeSH data available.


Related in: MedlinePlus