Limits...
Comparative metabolic fingerprinting of Gentiana rhodantha from different geographical origins using LC-UV-MS/MS and multivariate statistical analysis.

Pan Y, Zhang J, Shen T, Zhao YL, Wang YZ, Li WY - BMC Biochem. (2015)

Bottom Line: The most abundant mangiferin (82.21 mg/g) found in sample from Zunyi, Guizhou province.Furthermore, 64 samples according to their geographical origins, could be classified by partial least-squares discriminate analysis (PLS-DA) and nine compounds including two new compounds identified by mass spectrometry could be regarded as characteristic compounds for discriminating samples from different geographical origins.The developed method appears to be a useful tool for analysis of G. rhodantha, which could provide potential indicators for differentiation of different geographical origins.

View Article: PubMed Central - PubMed

Affiliation: Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, 2238, Beijing Road, Panlong District, Kunming, 650200, China. panyu412@hotmail.com.

ABSTRACT

Backgrounds: Gentiana rhodantha, a rich source of iridoids and polyphenols, is a traditional ethnomedicine widely used in China. Metabolic fingerprinting based on a LC-UV-MS/MS method was applied to explore the chemical markers for discrimination of G. rhodantha from different geographical origins.

Results: Targeted compounds were separated on a Shim-pack XR-ODS III (150 × 2.0 mm, 2.2 μm), with a mobile phase consisted of acetonitrile and 0.1% formic acid in water, under gradient elution. In quantitative analysis, all of the calibration curves showed good linear regression (R(2) < less than 0.9991) within the tested ranges, and accuracy ranged from 97.8% to 104.2% and the %RSD of precision (less than 3%) were all within the required limits. The most abundant mangiferin (82.21 mg/g) found in sample from Zunyi, Guizhou province. Furthermore, 64 samples according to their geographical origins, could be classified by partial least-squares discriminate analysis (PLS-DA) and nine compounds including two new compounds identified by mass spectrometry could be regarded as characteristic compounds for discriminating samples from different geographical origins.

Conclusions: The developed method appears to be a useful tool for analysis of G. rhodantha, which could provide potential indicators for differentiation of different geographical origins.

No MeSH data available.


Related in: MedlinePlus

Structures of the marker metabolites inG. rhodantha.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC4390080&req=5

Fig3: Structures of the marker metabolites inG. rhodantha.

Mentions: PLS-DA 3D scores plot (a) and Loading Bi-plot (b) of PLS-DA model on different geographical origins ofG. rhodantha. Detailed legend of Figure 3b: (DA1: southwest Guizhou; DA2: northeast Guizhou; DA3: Xilin DA4: northwest Yunnan; DA5: Kunming; DA6: southwest).


Comparative metabolic fingerprinting of Gentiana rhodantha from different geographical origins using LC-UV-MS/MS and multivariate statistical analysis.

Pan Y, Zhang J, Shen T, Zhao YL, Wang YZ, Li WY - BMC Biochem. (2015)

Structures of the marker metabolites inG. rhodantha.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4390080&req=5

Fig3: Structures of the marker metabolites inG. rhodantha.
Mentions: PLS-DA 3D scores plot (a) and Loading Bi-plot (b) of PLS-DA model on different geographical origins ofG. rhodantha. Detailed legend of Figure 3b: (DA1: southwest Guizhou; DA2: northeast Guizhou; DA3: Xilin DA4: northwest Yunnan; DA5: Kunming; DA6: southwest).

Bottom Line: The most abundant mangiferin (82.21 mg/g) found in sample from Zunyi, Guizhou province.Furthermore, 64 samples according to their geographical origins, could be classified by partial least-squares discriminate analysis (PLS-DA) and nine compounds including two new compounds identified by mass spectrometry could be regarded as characteristic compounds for discriminating samples from different geographical origins.The developed method appears to be a useful tool for analysis of G. rhodantha, which could provide potential indicators for differentiation of different geographical origins.

View Article: PubMed Central - PubMed

Affiliation: Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, 2238, Beijing Road, Panlong District, Kunming, 650200, China. panyu412@hotmail.com.

ABSTRACT

Backgrounds: Gentiana rhodantha, a rich source of iridoids and polyphenols, is a traditional ethnomedicine widely used in China. Metabolic fingerprinting based on a LC-UV-MS/MS method was applied to explore the chemical markers for discrimination of G. rhodantha from different geographical origins.

Results: Targeted compounds were separated on a Shim-pack XR-ODS III (150 × 2.0 mm, 2.2 μm), with a mobile phase consisted of acetonitrile and 0.1% formic acid in water, under gradient elution. In quantitative analysis, all of the calibration curves showed good linear regression (R(2) < less than 0.9991) within the tested ranges, and accuracy ranged from 97.8% to 104.2% and the %RSD of precision (less than 3%) were all within the required limits. The most abundant mangiferin (82.21 mg/g) found in sample from Zunyi, Guizhou province. Furthermore, 64 samples according to their geographical origins, could be classified by partial least-squares discriminate analysis (PLS-DA) and nine compounds including two new compounds identified by mass spectrometry could be regarded as characteristic compounds for discriminating samples from different geographical origins.

Conclusions: The developed method appears to be a useful tool for analysis of G. rhodantha, which could provide potential indicators for differentiation of different geographical origins.

No MeSH data available.


Related in: MedlinePlus