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In the wild hybridization of annual Datura species as unveiled by morphological and molecular comparisons.

Tsialtas IT, Patelou E, Kaloumenos NS, Mylona PV, Polidoros A, Menexes G, Eleftherohorinos IG - J Biol Res (Thessalon) (2014)

Bottom Line: The findings suggest that the natural hybridization of annual Datura species occurs.Extrapolating, this hybridization could be the first step for speciation.More possibly, it can alter population composition, its weediness and adaptability to local conditions.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Agriculture, Laboratory of Agronomy, Aristotle University of Thessaloniki, 541 24 Thessaloniki, Greece.

ABSTRACT

Background: The present work aimed to verify whether intermediate variants were natural crosses between Datura species (D. stramonium forms and D. ferox). Their existence has been long ago insinuated but has not been studied using morphological features and molecular tools. The variants differed in stem coloring, upper bearing forks, and fruit characters.

Results: Principal Components Analysis of 11 morphological characteristics showed that D. ferox and D. stramonium (forms stramonium and tatula) were quite different and the putative hybrids were intermittent. The D. ferox × D. stramonium f. tatula was closer to the latter of its parents. Sequencing analysis revealed identical amplified trnL intron in all variants and a 100% homology with D. stramonium accession number EU580984.1 suggested that this plastid cannot discern Datura variants. However, genomic analysis with URP markers indicated that the hybrids had >60% genetic makeup similarity with both parents suggesting that the intermediate variants were putative inter-specific hybrids. Moreover, the dendrogram stemmed from cluster analysis of the fingerprint profile of variants placed D. stramonium and D. ferox in different branches indicating their genetic differentiation from each other as well as from their hybrids.

Conclusions: The findings suggest that the natural hybridization of annual Datura species occurs. Extrapolating, this hybridization could be the first step for speciation. More possibly, it can alter population composition, its weediness and adaptability to local conditions.

No MeSH data available.


Related in: MedlinePlus

Upper bearing fork and capsule of the putative parents and their hybrids.
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Fig4: Upper bearing fork and capsule of the putative parents and their hybrids.

Mentions: Determinations were conducted in situ at Eukarpia site, where Datura population cohort was more homogenous. Six specimens, typical of each variant (Figure 4), were selected for measurements, which were conducted on the upper full expanded leaves (length, width, and length/width ratio), flowers (corolla and calyx length) and capsules (length, width, number of spines, short spine length, medium spine length, and long spine length). Lengths were measured using a HOREX electronic digital caliper (Helios-Preisser, Gammertingen, Germany). Stem color of the specimens was also recorded.Figure 4


In the wild hybridization of annual Datura species as unveiled by morphological and molecular comparisons.

Tsialtas IT, Patelou E, Kaloumenos NS, Mylona PV, Polidoros A, Menexes G, Eleftherohorinos IG - J Biol Res (Thessalon) (2014)

Upper bearing fork and capsule of the putative parents and their hybrids.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4389997&req=5

Fig4: Upper bearing fork and capsule of the putative parents and their hybrids.
Mentions: Determinations were conducted in situ at Eukarpia site, where Datura population cohort was more homogenous. Six specimens, typical of each variant (Figure 4), were selected for measurements, which were conducted on the upper full expanded leaves (length, width, and length/width ratio), flowers (corolla and calyx length) and capsules (length, width, number of spines, short spine length, medium spine length, and long spine length). Lengths were measured using a HOREX electronic digital caliper (Helios-Preisser, Gammertingen, Germany). Stem color of the specimens was also recorded.Figure 4

Bottom Line: The findings suggest that the natural hybridization of annual Datura species occurs.Extrapolating, this hybridization could be the first step for speciation.More possibly, it can alter population composition, its weediness and adaptability to local conditions.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Agriculture, Laboratory of Agronomy, Aristotle University of Thessaloniki, 541 24 Thessaloniki, Greece.

ABSTRACT

Background: The present work aimed to verify whether intermediate variants were natural crosses between Datura species (D. stramonium forms and D. ferox). Their existence has been long ago insinuated but has not been studied using morphological features and molecular tools. The variants differed in stem coloring, upper bearing forks, and fruit characters.

Results: Principal Components Analysis of 11 morphological characteristics showed that D. ferox and D. stramonium (forms stramonium and tatula) were quite different and the putative hybrids were intermittent. The D. ferox × D. stramonium f. tatula was closer to the latter of its parents. Sequencing analysis revealed identical amplified trnL intron in all variants and a 100% homology with D. stramonium accession number EU580984.1 suggested that this plastid cannot discern Datura variants. However, genomic analysis with URP markers indicated that the hybrids had >60% genetic makeup similarity with both parents suggesting that the intermediate variants were putative inter-specific hybrids. Moreover, the dendrogram stemmed from cluster analysis of the fingerprint profile of variants placed D. stramonium and D. ferox in different branches indicating their genetic differentiation from each other as well as from their hybrids.

Conclusions: The findings suggest that the natural hybridization of annual Datura species occurs. Extrapolating, this hybridization could be the first step for speciation. More possibly, it can alter population composition, its weediness and adaptability to local conditions.

No MeSH data available.


Related in: MedlinePlus