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In the wild hybridization of annual Datura species as unveiled by morphological and molecular comparisons.

Tsialtas IT, Patelou E, Kaloumenos NS, Mylona PV, Polidoros A, Menexes G, Eleftherohorinos IG - J Biol Res (Thessalon) (2014)

Bottom Line: The findings suggest that the natural hybridization of annual Datura species occurs.Extrapolating, this hybridization could be the first step for speciation.More possibly, it can alter population composition, its weediness and adaptability to local conditions.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Agriculture, Laboratory of Agronomy, Aristotle University of Thessaloniki, 541 24 Thessaloniki, Greece.

ABSTRACT

Background: The present work aimed to verify whether intermediate variants were natural crosses between Datura species (D. stramonium forms and D. ferox). Their existence has been long ago insinuated but has not been studied using morphological features and molecular tools. The variants differed in stem coloring, upper bearing forks, and fruit characters.

Results: Principal Components Analysis of 11 morphological characteristics showed that D. ferox and D. stramonium (forms stramonium and tatula) were quite different and the putative hybrids were intermittent. The D. ferox × D. stramonium f. tatula was closer to the latter of its parents. Sequencing analysis revealed identical amplified trnL intron in all variants and a 100% homology with D. stramonium accession number EU580984.1 suggested that this plastid cannot discern Datura variants. However, genomic analysis with URP markers indicated that the hybrids had >60% genetic makeup similarity with both parents suggesting that the intermediate variants were putative inter-specific hybrids. Moreover, the dendrogram stemmed from cluster analysis of the fingerprint profile of variants placed D. stramonium and D. ferox in different branches indicating their genetic differentiation from each other as well as from their hybrids.

Conclusions: The findings suggest that the natural hybridization of annual Datura species occurs. Extrapolating, this hybridization could be the first step for speciation. More possibly, it can alter population composition, its weediness and adaptability to local conditions.

No MeSH data available.


Dendrogram showing relationships of theDaturavariants according to their fingerprinting profile. The tree was constructed from the Jaccard dissimilarity matrix using the UPGMA method of the MEGA5 software. S: D. stramonium; F: D. ferox; A: D. ferox × f. tatula; B: D. ferox × f. stramonium.
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Fig3: Dendrogram showing relationships of theDaturavariants according to their fingerprinting profile. The tree was constructed from the Jaccard dissimilarity matrix using the UPGMA method of the MEGA5 software. S: D. stramonium; F: D. ferox; A: D. ferox × f. tatula; B: D. ferox × f. stramonium.

Mentions: The dendrogram obtained from the cluster analysis of the examined fingerprint profile of the variants showed grouping of the putative hybrids in one cluster (Figure 3). The position of D. stramonium and D. ferox in different branches of the tree indicates their genetic differentiation from each other as well as from the putative hybrids.Figure 3


In the wild hybridization of annual Datura species as unveiled by morphological and molecular comparisons.

Tsialtas IT, Patelou E, Kaloumenos NS, Mylona PV, Polidoros A, Menexes G, Eleftherohorinos IG - J Biol Res (Thessalon) (2014)

Dendrogram showing relationships of theDaturavariants according to their fingerprinting profile. The tree was constructed from the Jaccard dissimilarity matrix using the UPGMA method of the MEGA5 software. S: D. stramonium; F: D. ferox; A: D. ferox × f. tatula; B: D. ferox × f. stramonium.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4389997&req=5

Fig3: Dendrogram showing relationships of theDaturavariants according to their fingerprinting profile. The tree was constructed from the Jaccard dissimilarity matrix using the UPGMA method of the MEGA5 software. S: D. stramonium; F: D. ferox; A: D. ferox × f. tatula; B: D. ferox × f. stramonium.
Mentions: The dendrogram obtained from the cluster analysis of the examined fingerprint profile of the variants showed grouping of the putative hybrids in one cluster (Figure 3). The position of D. stramonium and D. ferox in different branches of the tree indicates their genetic differentiation from each other as well as from the putative hybrids.Figure 3

Bottom Line: The findings suggest that the natural hybridization of annual Datura species occurs.Extrapolating, this hybridization could be the first step for speciation.More possibly, it can alter population composition, its weediness and adaptability to local conditions.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Agriculture, Laboratory of Agronomy, Aristotle University of Thessaloniki, 541 24 Thessaloniki, Greece.

ABSTRACT

Background: The present work aimed to verify whether intermediate variants were natural crosses between Datura species (D. stramonium forms and D. ferox). Their existence has been long ago insinuated but has not been studied using morphological features and molecular tools. The variants differed in stem coloring, upper bearing forks, and fruit characters.

Results: Principal Components Analysis of 11 morphological characteristics showed that D. ferox and D. stramonium (forms stramonium and tatula) were quite different and the putative hybrids were intermittent. The D. ferox × D. stramonium f. tatula was closer to the latter of its parents. Sequencing analysis revealed identical amplified trnL intron in all variants and a 100% homology with D. stramonium accession number EU580984.1 suggested that this plastid cannot discern Datura variants. However, genomic analysis with URP markers indicated that the hybrids had >60% genetic makeup similarity with both parents suggesting that the intermediate variants were putative inter-specific hybrids. Moreover, the dendrogram stemmed from cluster analysis of the fingerprint profile of variants placed D. stramonium and D. ferox in different branches indicating their genetic differentiation from each other as well as from their hybrids.

Conclusions: The findings suggest that the natural hybridization of annual Datura species occurs. Extrapolating, this hybridization could be the first step for speciation. More possibly, it can alter population composition, its weediness and adaptability to local conditions.

No MeSH data available.