Limits...
In the wild hybridization of annual Datura species as unveiled by morphological and molecular comparisons.

Tsialtas IT, Patelou E, Kaloumenos NS, Mylona PV, Polidoros A, Menexes G, Eleftherohorinos IG - J Biol Res (Thessalon) (2014)

Bottom Line: The findings suggest that the natural hybridization of annual Datura species occurs.Extrapolating, this hybridization could be the first step for speciation.More possibly, it can alter population composition, its weediness and adaptability to local conditions.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Agriculture, Laboratory of Agronomy, Aristotle University of Thessaloniki, 541 24 Thessaloniki, Greece.

ABSTRACT

Background: The present work aimed to verify whether intermediate variants were natural crosses between Datura species (D. stramonium forms and D. ferox). Their existence has been long ago insinuated but has not been studied using morphological features and molecular tools. The variants differed in stem coloring, upper bearing forks, and fruit characters.

Results: Principal Components Analysis of 11 morphological characteristics showed that D. ferox and D. stramonium (forms stramonium and tatula) were quite different and the putative hybrids were intermittent. The D. ferox × D. stramonium f. tatula was closer to the latter of its parents. Sequencing analysis revealed identical amplified trnL intron in all variants and a 100% homology with D. stramonium accession number EU580984.1 suggested that this plastid cannot discern Datura variants. However, genomic analysis with URP markers indicated that the hybrids had >60% genetic makeup similarity with both parents suggesting that the intermediate variants were putative inter-specific hybrids. Moreover, the dendrogram stemmed from cluster analysis of the fingerprint profile of variants placed D. stramonium and D. ferox in different branches indicating their genetic differentiation from each other as well as from their hybrids.

Conclusions: The findings suggest that the natural hybridization of annual Datura species occurs. Extrapolating, this hybridization could be the first step for speciation. More possibly, it can alter population composition, its weediness and adaptability to local conditions.

No MeSH data available.


Related in: MedlinePlus

PCR products of theDaturavariants amplified using the URP primers. Each lane is labeled according to the template and primer used for amplification. Letters represent variants followed by dot and a number that represents primer. Variant labels are S: D. stramonium; F: D. ferox; A: putative D. ferox × f. tatula; B: putative D. ferox × f. stramonium. URP primers used (1–12) are shown in Table 5. Molecular marker is the 1 kb DNA ladder.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC4389997&req=5

Fig2: PCR products of theDaturavariants amplified using the URP primers. Each lane is labeled according to the template and primer used for amplification. Letters represent variants followed by dot and a number that represents primer. Variant labels are S: D. stramonium; F: D. ferox; A: putative D. ferox × f. tatula; B: putative D. ferox × f. stramonium. URP primers used (1–12) are shown in Table 5. Molecular marker is the 1 kb DNA ladder.

Mentions: The PCR fingerprinting, using all the primers and DNA, detected 63 clear and scorable bands in the samples including D. stramonium, D. ferox, and the two putative inter-specific hybrids (Figure 2). The band patterns of the D. stramonium forms were identical and only results from one form are shown. The URP primers produced multiple bands in all variants, varying in size from about 100 bp to more than 3000 bp. Polymorphic as well as monomorphic bands were revealed with 11 from the 12 URP primers. Only primer URP13R (number 7 in Figure 2) produced a single monomorphic band in all samples. Out of 63 scorable bands, 37 bands (58.73%) were found to be polymorphic (present in one to three variants) while 26 bands (41.27%) were monomorphic (present in all variants). Totally, 20 polymorphic bands (31.75%) were present in only one variant, while six were present in two (9.5%) and 11 in three variants (17.5%).Figure 2


In the wild hybridization of annual Datura species as unveiled by morphological and molecular comparisons.

Tsialtas IT, Patelou E, Kaloumenos NS, Mylona PV, Polidoros A, Menexes G, Eleftherohorinos IG - J Biol Res (Thessalon) (2014)

PCR products of theDaturavariants amplified using the URP primers. Each lane is labeled according to the template and primer used for amplification. Letters represent variants followed by dot and a number that represents primer. Variant labels are S: D. stramonium; F: D. ferox; A: putative D. ferox × f. tatula; B: putative D. ferox × f. stramonium. URP primers used (1–12) are shown in Table 5. Molecular marker is the 1 kb DNA ladder.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4389997&req=5

Fig2: PCR products of theDaturavariants amplified using the URP primers. Each lane is labeled according to the template and primer used for amplification. Letters represent variants followed by dot and a number that represents primer. Variant labels are S: D. stramonium; F: D. ferox; A: putative D. ferox × f. tatula; B: putative D. ferox × f. stramonium. URP primers used (1–12) are shown in Table 5. Molecular marker is the 1 kb DNA ladder.
Mentions: The PCR fingerprinting, using all the primers and DNA, detected 63 clear and scorable bands in the samples including D. stramonium, D. ferox, and the two putative inter-specific hybrids (Figure 2). The band patterns of the D. stramonium forms were identical and only results from one form are shown. The URP primers produced multiple bands in all variants, varying in size from about 100 bp to more than 3000 bp. Polymorphic as well as monomorphic bands were revealed with 11 from the 12 URP primers. Only primer URP13R (number 7 in Figure 2) produced a single monomorphic band in all samples. Out of 63 scorable bands, 37 bands (58.73%) were found to be polymorphic (present in one to three variants) while 26 bands (41.27%) were monomorphic (present in all variants). Totally, 20 polymorphic bands (31.75%) were present in only one variant, while six were present in two (9.5%) and 11 in three variants (17.5%).Figure 2

Bottom Line: The findings suggest that the natural hybridization of annual Datura species occurs.Extrapolating, this hybridization could be the first step for speciation.More possibly, it can alter population composition, its weediness and adaptability to local conditions.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Agriculture, Laboratory of Agronomy, Aristotle University of Thessaloniki, 541 24 Thessaloniki, Greece.

ABSTRACT

Background: The present work aimed to verify whether intermediate variants were natural crosses between Datura species (D. stramonium forms and D. ferox). Their existence has been long ago insinuated but has not been studied using morphological features and molecular tools. The variants differed in stem coloring, upper bearing forks, and fruit characters.

Results: Principal Components Analysis of 11 morphological characteristics showed that D. ferox and D. stramonium (forms stramonium and tatula) were quite different and the putative hybrids were intermittent. The D. ferox × D. stramonium f. tatula was closer to the latter of its parents. Sequencing analysis revealed identical amplified trnL intron in all variants and a 100% homology with D. stramonium accession number EU580984.1 suggested that this plastid cannot discern Datura variants. However, genomic analysis with URP markers indicated that the hybrids had >60% genetic makeup similarity with both parents suggesting that the intermediate variants were putative inter-specific hybrids. Moreover, the dendrogram stemmed from cluster analysis of the fingerprint profile of variants placed D. stramonium and D. ferox in different branches indicating their genetic differentiation from each other as well as from their hybrids.

Conclusions: The findings suggest that the natural hybridization of annual Datura species occurs. Extrapolating, this hybridization could be the first step for speciation. More possibly, it can alter population composition, its weediness and adaptability to local conditions.

No MeSH data available.


Related in: MedlinePlus