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In the wild hybridization of annual Datura species as unveiled by morphological and molecular comparisons.

Tsialtas IT, Patelou E, Kaloumenos NS, Mylona PV, Polidoros A, Menexes G, Eleftherohorinos IG - J Biol Res (Thessalon) (2014)

Bottom Line: The findings suggest that the natural hybridization of annual Datura species occurs.Extrapolating, this hybridization could be the first step for speciation.More possibly, it can alter population composition, its weediness and adaptability to local conditions.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Agriculture, Laboratory of Agronomy, Aristotle University of Thessaloniki, 541 24 Thessaloniki, Greece.

ABSTRACT

Background: The present work aimed to verify whether intermediate variants were natural crosses between Datura species (D. stramonium forms and D. ferox). Their existence has been long ago insinuated but has not been studied using morphological features and molecular tools. The variants differed in stem coloring, upper bearing forks, and fruit characters.

Results: Principal Components Analysis of 11 morphological characteristics showed that D. ferox and D. stramonium (forms stramonium and tatula) were quite different and the putative hybrids were intermittent. The D. ferox × D. stramonium f. tatula was closer to the latter of its parents. Sequencing analysis revealed identical amplified trnL intron in all variants and a 100% homology with D. stramonium accession number EU580984.1 suggested that this plastid cannot discern Datura variants. However, genomic analysis with URP markers indicated that the hybrids had >60% genetic makeup similarity with both parents suggesting that the intermediate variants were putative inter-specific hybrids. Moreover, the dendrogram stemmed from cluster analysis of the fingerprint profile of variants placed D. stramonium and D. ferox in different branches indicating their genetic differentiation from each other as well as from their hybrids.

Conclusions: The findings suggest that the natural hybridization of annual Datura species occurs. Extrapolating, this hybridization could be the first step for speciation. More possibly, it can alter population composition, its weediness and adaptability to local conditions.

No MeSH data available.


Plot ofDaturaindividuals by first and second factorial scores derived from PCA. ● D. ferox, ■ f. stramonium, ○ f. tatula, ▲ D. ferox × f. stramonium and ▼ D. ferox × f. tatula.
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Fig1: Plot ofDaturaindividuals by first and second factorial scores derived from PCA. ● D. ferox, ■ f. stramonium, ○ f. tatula, ▲ D. ferox × f. stramonium and ▼ D. ferox × f. tatula.

Mentions: The projection of the 30 plant individuals on the 1 × 2 factorial plane derived from PCA indicated that the first component PC1 clearly separated the D. ferox from f. stramonium individuals while D. ferox × f. stramonium plants were projected around the origin almost in the middle of their two parents (Figure 1). The second component PC2 distinguished D. ferox and f. stramonium individuals from the f. tatula and D. ferox × f. tatula plants. The D. ferox × f. tatula variants were most similar with one of their parents, f. tatula. The individuals of D. ferox, f. stramonium, and D. ferox × f. stramonium showed greater variability along the PC1 axis than the f. tatula and D. ferox × f. tatula plants, which had very little variability on the same axis; in contrast they showed some variability along the PC2 axis.Figure 1


In the wild hybridization of annual Datura species as unveiled by morphological and molecular comparisons.

Tsialtas IT, Patelou E, Kaloumenos NS, Mylona PV, Polidoros A, Menexes G, Eleftherohorinos IG - J Biol Res (Thessalon) (2014)

Plot ofDaturaindividuals by first and second factorial scores derived from PCA. ● D. ferox, ■ f. stramonium, ○ f. tatula, ▲ D. ferox × f. stramonium and ▼ D. ferox × f. tatula.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4389997&req=5

Fig1: Plot ofDaturaindividuals by first and second factorial scores derived from PCA. ● D. ferox, ■ f. stramonium, ○ f. tatula, ▲ D. ferox × f. stramonium and ▼ D. ferox × f. tatula.
Mentions: The projection of the 30 plant individuals on the 1 × 2 factorial plane derived from PCA indicated that the first component PC1 clearly separated the D. ferox from f. stramonium individuals while D. ferox × f. stramonium plants were projected around the origin almost in the middle of their two parents (Figure 1). The second component PC2 distinguished D. ferox and f. stramonium individuals from the f. tatula and D. ferox × f. tatula plants. The D. ferox × f. tatula variants were most similar with one of their parents, f. tatula. The individuals of D. ferox, f. stramonium, and D. ferox × f. stramonium showed greater variability along the PC1 axis than the f. tatula and D. ferox × f. tatula plants, which had very little variability on the same axis; in contrast they showed some variability along the PC2 axis.Figure 1

Bottom Line: The findings suggest that the natural hybridization of annual Datura species occurs.Extrapolating, this hybridization could be the first step for speciation.More possibly, it can alter population composition, its weediness and adaptability to local conditions.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Agriculture, Laboratory of Agronomy, Aristotle University of Thessaloniki, 541 24 Thessaloniki, Greece.

ABSTRACT

Background: The present work aimed to verify whether intermediate variants were natural crosses between Datura species (D. stramonium forms and D. ferox). Their existence has been long ago insinuated but has not been studied using morphological features and molecular tools. The variants differed in stem coloring, upper bearing forks, and fruit characters.

Results: Principal Components Analysis of 11 morphological characteristics showed that D. ferox and D. stramonium (forms stramonium and tatula) were quite different and the putative hybrids were intermittent. The D. ferox × D. stramonium f. tatula was closer to the latter of its parents. Sequencing analysis revealed identical amplified trnL intron in all variants and a 100% homology with D. stramonium accession number EU580984.1 suggested that this plastid cannot discern Datura variants. However, genomic analysis with URP markers indicated that the hybrids had >60% genetic makeup similarity with both parents suggesting that the intermediate variants were putative inter-specific hybrids. Moreover, the dendrogram stemmed from cluster analysis of the fingerprint profile of variants placed D. stramonium and D. ferox in different branches indicating their genetic differentiation from each other as well as from their hybrids.

Conclusions: The findings suggest that the natural hybridization of annual Datura species occurs. Extrapolating, this hybridization could be the first step for speciation. More possibly, it can alter population composition, its weediness and adaptability to local conditions.

No MeSH data available.