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Anxiolytic and antidepressant profile of the methanolic extract of Piper nigrum fruits in beta-amyloid (1-42) rat model of Alzheimer's disease.

Hritcu L, Noumedem JA, Cioanca O, Hancianu M, Postu P, Mihasan M - Behav Brain Funct (2015)

Bottom Line: Also, the antioxidant activity in the amygdala was assessed using superoxide dismutase, glutathione peroxidase and catalase specific activities, the total content of the reduced glutathione, protein carbonyl and malondialdehyde levels.F values for which p < 0.05 were regarded as statistically significant.Administration of the methanolic extract significantly exhibited anxiolytic- and antidepressant-like effects and also antioxidant potential.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Alexandru Ioan Cuza University, Bd. Carol I, No.11, Iasi, 700506, Romania. hritcu@uaic.ro.

ABSTRACT

Background: Piper nigrum L. (Piperaceae) is employed in traditional medicine of many countries as analgesic, antiinflammatory, anticonvulsant, antioxidant, antidepressant and cognitive-enhancing agent. This study was undertaken in order to evaluate the possible anxiolytic, antidepressant and antioxidant properties of the methanolic extract of Piper nigrum fruits in beta-amyloid (1-42) rat model of Alzheimer's disease.

Methods: The anxiolytic- and antidepressant-like effects of the methanolic extract were studied by means of in vivo (elevated plus-maze and forced swimming tests) approaches. Also, the antioxidant activity in the amygdala was assessed using superoxide dismutase, glutathione peroxidase and catalase specific activities, the total content of the reduced glutathione, protein carbonyl and malondialdehyde levels. Statistical analyses were performed using one-way analysis of variance (ANOVA). Significant differences were determined by Tukey's post hoc test. F values for which p < 0.05 were regarded as statistically significant. Pearson's correlation coefficient and regression analysis were used in order to evaluate the connection between behavioral measures, the antioxidant defence and lipid peroxidation.

Results: The beta-amyloid (1-42)-treated rats exhibited the following: decrease of the exploratory activity, the percentage of the time spent and the number of entries in the open arm within elevated plus-maze test and decrease of swimming time and increase of immobility time within forced swimming test. Administration of the methanolic extract significantly exhibited anxiolytic- and antidepressant-like effects and also antioxidant potential.

Conclusions: Taken together, our results suggest that the methanolic extract ameliorates beta-amyloid (1-42)-induced anxiety and depression by attenuation of the oxidative stress in the rat amygdala.

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Related in: MedlinePlus

Effects of the methanolic extract ofPiper nigrumfruits (50 and 100 mg/kg) on SOD (a), GPX (b) and CAT (c) specific activities, on total content of reduced GSH (d), protein carbonyl (e) and MDA (f) levels in the Aβ (1–42)-treated rats. Values are means + S.E.M. (n = 10 animals per group). For Turkey’s post hoc analysis - #Aβ (1–42) vs. Aβ (1–42)+P50: p<0.0001 and ##Aβ (1–42) vs. Aβ (1–42)+P100: p<0.0001 (a), #Aβ (1–42) vs. Aβ (1–42)+P50: p<0.0001 and ##Aβ (1–42) vs. Aβ (1–42)+P100: p<0.0001 (b), #Aβ (1–42) and Aβ (1–42)+P50 groups (p<0.001) and ##Aβ (1–42) vs. Aβ (1–42)+P100: p<0.0001(c), ##Aβ (1–42) vs. Aβ (1–42)+P100: p<0.01 (d), #Aβ (1–42) vs. Aβ (1–42)+P50: p<0.0001 and ##Aβ (1–42) vs. Aβ (1–42)+P100: p<0.0001 (e) and #Aβ (1–42) vs. Aβ (1–42)+P50: p<0.0001 and ##Aβ (1–42) vs. Aβ (1–42)+P100: p<0.0001 (f).
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Fig4: Effects of the methanolic extract ofPiper nigrumfruits (50 and 100 mg/kg) on SOD (a), GPX (b) and CAT (c) specific activities, on total content of reduced GSH (d), protein carbonyl (e) and MDA (f) levels in the Aβ (1–42)-treated rats. Values are means + S.E.M. (n = 10 animals per group). For Turkey’s post hoc analysis - #Aβ (1–42) vs. Aβ (1–42)+P50: p<0.0001 and ##Aβ (1–42) vs. Aβ (1–42)+P100: p<0.0001 (a), #Aβ (1–42) vs. Aβ (1–42)+P50: p<0.0001 and ##Aβ (1–42) vs. Aβ (1–42)+P100: p<0.0001 (b), #Aβ (1–42) and Aβ (1–42)+P50 groups (p<0.001) and ##Aβ (1–42) vs. Aβ (1–42)+P100: p<0.0001(c), ##Aβ (1–42) vs. Aβ (1–42)+P100: p<0.01 (d), #Aβ (1–42) vs. Aβ (1–42)+P50: p<0.0001 and ##Aβ (1–42) vs. Aβ (1–42)+P100: p<0.0001 (e) and #Aβ (1–42) vs. Aβ (1–42)+P50: p<0.0001 and ##Aβ (1–42) vs. Aβ (1–42)+P100: p<0.0001 (f).

Mentions: For SOD, GPX and CAT specific activities estimated in the rat amygdala homogenates, ANOVA revealed a significant overall differences between all groups for SOD (F(3,36) = 20.54, p < 0.0001) (Figure 4a), GPX (F(3, 36) = 66.48, p < 0.0001) (Figure 4b) and CAT (F(3, 36) = 12.77, p < 0.0001) (Figure 4c) specific activities. Both doses of the methanolic extract, but especially 100 mg/kg, significantly decreased the specific activities of SOD, GPX and CAT of Aβ (1–42)-treated groups as compared to Aβ (1–42) alone-treated group. Additionally, Tukey’s post hoc analysis revealed significant differences between Aβ (1–42) and Aβ (1–42) + P50 groups (p < 0.0001) and Aβ (1–42) and Aβ (1–42) + P100 groups (p < 0.0001) for SOD specific activity, between Aβ(1–42) and Aβ (1–42) + P50 groups (p < 0.0001) and Aβ (1–42) and Aβ (1–42) + P100 groups (p < 0.0001) for GPX specific activity and between Aβ (1–42) + P50 groups (p < 0.0001), Aβ (1–42) and Aβ (1–42) + P100 groups (p < 0.0001) for CAT specific activity.Figure 4


Anxiolytic and antidepressant profile of the methanolic extract of Piper nigrum fruits in beta-amyloid (1-42) rat model of Alzheimer's disease.

Hritcu L, Noumedem JA, Cioanca O, Hancianu M, Postu P, Mihasan M - Behav Brain Funct (2015)

Effects of the methanolic extract ofPiper nigrumfruits (50 and 100 mg/kg) on SOD (a), GPX (b) and CAT (c) specific activities, on total content of reduced GSH (d), protein carbonyl (e) and MDA (f) levels in the Aβ (1–42)-treated rats. Values are means + S.E.M. (n = 10 animals per group). For Turkey’s post hoc analysis - #Aβ (1–42) vs. Aβ (1–42)+P50: p<0.0001 and ##Aβ (1–42) vs. Aβ (1–42)+P100: p<0.0001 (a), #Aβ (1–42) vs. Aβ (1–42)+P50: p<0.0001 and ##Aβ (1–42) vs. Aβ (1–42)+P100: p<0.0001 (b), #Aβ (1–42) and Aβ (1–42)+P50 groups (p<0.001) and ##Aβ (1–42) vs. Aβ (1–42)+P100: p<0.0001(c), ##Aβ (1–42) vs. Aβ (1–42)+P100: p<0.01 (d), #Aβ (1–42) vs. Aβ (1–42)+P50: p<0.0001 and ##Aβ (1–42) vs. Aβ (1–42)+P100: p<0.0001 (e) and #Aβ (1–42) vs. Aβ (1–42)+P50: p<0.0001 and ##Aβ (1–42) vs. Aβ (1–42)+P100: p<0.0001 (f).
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Related In: Results  -  Collection

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Fig4: Effects of the methanolic extract ofPiper nigrumfruits (50 and 100 mg/kg) on SOD (a), GPX (b) and CAT (c) specific activities, on total content of reduced GSH (d), protein carbonyl (e) and MDA (f) levels in the Aβ (1–42)-treated rats. Values are means + S.E.M. (n = 10 animals per group). For Turkey’s post hoc analysis - #Aβ (1–42) vs. Aβ (1–42)+P50: p<0.0001 and ##Aβ (1–42) vs. Aβ (1–42)+P100: p<0.0001 (a), #Aβ (1–42) vs. Aβ (1–42)+P50: p<0.0001 and ##Aβ (1–42) vs. Aβ (1–42)+P100: p<0.0001 (b), #Aβ (1–42) and Aβ (1–42)+P50 groups (p<0.001) and ##Aβ (1–42) vs. Aβ (1–42)+P100: p<0.0001(c), ##Aβ (1–42) vs. Aβ (1–42)+P100: p<0.01 (d), #Aβ (1–42) vs. Aβ (1–42)+P50: p<0.0001 and ##Aβ (1–42) vs. Aβ (1–42)+P100: p<0.0001 (e) and #Aβ (1–42) vs. Aβ (1–42)+P50: p<0.0001 and ##Aβ (1–42) vs. Aβ (1–42)+P100: p<0.0001 (f).
Mentions: For SOD, GPX and CAT specific activities estimated in the rat amygdala homogenates, ANOVA revealed a significant overall differences between all groups for SOD (F(3,36) = 20.54, p < 0.0001) (Figure 4a), GPX (F(3, 36) = 66.48, p < 0.0001) (Figure 4b) and CAT (F(3, 36) = 12.77, p < 0.0001) (Figure 4c) specific activities. Both doses of the methanolic extract, but especially 100 mg/kg, significantly decreased the specific activities of SOD, GPX and CAT of Aβ (1–42)-treated groups as compared to Aβ (1–42) alone-treated group. Additionally, Tukey’s post hoc analysis revealed significant differences between Aβ (1–42) and Aβ (1–42) + P50 groups (p < 0.0001) and Aβ (1–42) and Aβ (1–42) + P100 groups (p < 0.0001) for SOD specific activity, between Aβ(1–42) and Aβ (1–42) + P50 groups (p < 0.0001) and Aβ (1–42) and Aβ (1–42) + P100 groups (p < 0.0001) for GPX specific activity and between Aβ (1–42) + P50 groups (p < 0.0001), Aβ (1–42) and Aβ (1–42) + P100 groups (p < 0.0001) for CAT specific activity.Figure 4

Bottom Line: Also, the antioxidant activity in the amygdala was assessed using superoxide dismutase, glutathione peroxidase and catalase specific activities, the total content of the reduced glutathione, protein carbonyl and malondialdehyde levels.F values for which p < 0.05 were regarded as statistically significant.Administration of the methanolic extract significantly exhibited anxiolytic- and antidepressant-like effects and also antioxidant potential.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Alexandru Ioan Cuza University, Bd. Carol I, No.11, Iasi, 700506, Romania. hritcu@uaic.ro.

ABSTRACT

Background: Piper nigrum L. (Piperaceae) is employed in traditional medicine of many countries as analgesic, antiinflammatory, anticonvulsant, antioxidant, antidepressant and cognitive-enhancing agent. This study was undertaken in order to evaluate the possible anxiolytic, antidepressant and antioxidant properties of the methanolic extract of Piper nigrum fruits in beta-amyloid (1-42) rat model of Alzheimer's disease.

Methods: The anxiolytic- and antidepressant-like effects of the methanolic extract were studied by means of in vivo (elevated plus-maze and forced swimming tests) approaches. Also, the antioxidant activity in the amygdala was assessed using superoxide dismutase, glutathione peroxidase and catalase specific activities, the total content of the reduced glutathione, protein carbonyl and malondialdehyde levels. Statistical analyses were performed using one-way analysis of variance (ANOVA). Significant differences were determined by Tukey's post hoc test. F values for which p < 0.05 were regarded as statistically significant. Pearson's correlation coefficient and regression analysis were used in order to evaluate the connection between behavioral measures, the antioxidant defence and lipid peroxidation.

Results: The beta-amyloid (1-42)-treated rats exhibited the following: decrease of the exploratory activity, the percentage of the time spent and the number of entries in the open arm within elevated plus-maze test and decrease of swimming time and increase of immobility time within forced swimming test. Administration of the methanolic extract significantly exhibited anxiolytic- and antidepressant-like effects and also antioxidant potential.

Conclusions: Taken together, our results suggest that the methanolic extract ameliorates beta-amyloid (1-42)-induced anxiety and depression by attenuation of the oxidative stress in the rat amygdala.

Show MeSH
Related in: MedlinePlus