Limits...
Therapeutic effect of Agaricus brasiliensis on phenylhydrazine-induced neonatal jaundice in rats.

Zhang L, Yuan B, Wang H, Gao Y - Biomed Res Int (2015)

Bottom Line: Administration of ABE dose-dependently reduced the elevated bilirubin level induced by phenylhydrazine.It can be somewhat supported from the results of in vitro bilirubin degradation experiment.ABE treatment also reduced the total antioxidant status (TAOS), cascade O2(-)/SOD, level of NF-κB protein, and adrenomedullin (AM).

View Article: PubMed Central - PubMed

Affiliation: Department of Pediatrics, Second Hospital of Xi'an Jiaotong University, Xi'an 710004, China.

ABSTRACT
The present study was designed to investigate the effect of Agaricus brasiliensis extract (ABE) on phenylhydrazine-induced neonatal jaundice in rats. Administration of ABE dose-dependently reduced the elevated bilirubin level induced by phenylhydrazine. It can be somewhat supported from the results of in vitro bilirubin degradation experiment. ABE treatment also reduced the total antioxidant status (TAOS), cascade O2(-)/SOD, level of NF-κB protein, and adrenomedullin (AM). Overall, the results of this study demonstrated that Agaricus brasiliensis extract may be beneficial to reducing bilirubin level without causing hepatotoxicity in neonatal jaundice.

Show MeSH

Related in: MedlinePlus

Effect of ABE on cascade of O2−/SOD values. Values are shown as means ± SEM. *P < 0.05 versus phenylhydrazine, **P < 0.01 versus phenylhydrazine group (Pdz: phenylhydrazine).
© Copyright Policy - open-access
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4389989&req=5

fig3: Effect of ABE on cascade of O2−/SOD values. Values are shown as means ± SEM. *P < 0.05 versus phenylhydrazine, **P < 0.01 versus phenylhydrazine group (Pdz: phenylhydrazine).

Mentions: Several studies have shown that the antioxidant defense system is altered during pregnancy. Exposure to oxidative stress may result in excessive bilirubin production that, when combined with diminished conjugation capacity, severely exacerbates the potential for extreme hyperbilirubinemia [24]. Neonatal erythrocytes are prone to oxidative damage due to their unsaturated membrane lipids [25]. In this study, phenylhydrazine administration increased TAOS activity (Table 3). We measured TAOS activity as an indirect indication of the formation of O2− and other oxidant species. Those in the ABE-50- and ABE-100-treated groups were significantly lower than those in the phenylhydrazine-treated group (P < 0.05 and P < 0.01, resp.). Moreover, significantly higher cascade of O2−/SOD values was measured in the phenylhydrazine group (P < 0.01) compared to the control group. ABE-100 suppressed phenylhydrazine and induced the higher cascade of O2−/SOD values. Taken together, these results clearly indicated that oxidative stress was generated in erythrocyte of neonatal jaundiced mice. ABE treatment reversed these adverse effects (Figure 3).


Therapeutic effect of Agaricus brasiliensis on phenylhydrazine-induced neonatal jaundice in rats.

Zhang L, Yuan B, Wang H, Gao Y - Biomed Res Int (2015)

Effect of ABE on cascade of O2−/SOD values. Values are shown as means ± SEM. *P < 0.05 versus phenylhydrazine, **P < 0.01 versus phenylhydrazine group (Pdz: phenylhydrazine).
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4389989&req=5

fig3: Effect of ABE on cascade of O2−/SOD values. Values are shown as means ± SEM. *P < 0.05 versus phenylhydrazine, **P < 0.01 versus phenylhydrazine group (Pdz: phenylhydrazine).
Mentions: Several studies have shown that the antioxidant defense system is altered during pregnancy. Exposure to oxidative stress may result in excessive bilirubin production that, when combined with diminished conjugation capacity, severely exacerbates the potential for extreme hyperbilirubinemia [24]. Neonatal erythrocytes are prone to oxidative damage due to their unsaturated membrane lipids [25]. In this study, phenylhydrazine administration increased TAOS activity (Table 3). We measured TAOS activity as an indirect indication of the formation of O2− and other oxidant species. Those in the ABE-50- and ABE-100-treated groups were significantly lower than those in the phenylhydrazine-treated group (P < 0.05 and P < 0.01, resp.). Moreover, significantly higher cascade of O2−/SOD values was measured in the phenylhydrazine group (P < 0.01) compared to the control group. ABE-100 suppressed phenylhydrazine and induced the higher cascade of O2−/SOD values. Taken together, these results clearly indicated that oxidative stress was generated in erythrocyte of neonatal jaundiced mice. ABE treatment reversed these adverse effects (Figure 3).

Bottom Line: Administration of ABE dose-dependently reduced the elevated bilirubin level induced by phenylhydrazine.It can be somewhat supported from the results of in vitro bilirubin degradation experiment.ABE treatment also reduced the total antioxidant status (TAOS), cascade O2(-)/SOD, level of NF-κB protein, and adrenomedullin (AM).

View Article: PubMed Central - PubMed

Affiliation: Department of Pediatrics, Second Hospital of Xi'an Jiaotong University, Xi'an 710004, China.

ABSTRACT
The present study was designed to investigate the effect of Agaricus brasiliensis extract (ABE) on phenylhydrazine-induced neonatal jaundice in rats. Administration of ABE dose-dependently reduced the elevated bilirubin level induced by phenylhydrazine. It can be somewhat supported from the results of in vitro bilirubin degradation experiment. ABE treatment also reduced the total antioxidant status (TAOS), cascade O2(-)/SOD, level of NF-κB protein, and adrenomedullin (AM). Overall, the results of this study demonstrated that Agaricus brasiliensis extract may be beneficial to reducing bilirubin level without causing hepatotoxicity in neonatal jaundice.

Show MeSH
Related in: MedlinePlus