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Therapeutic effect of Agaricus brasiliensis on phenylhydrazine-induced neonatal jaundice in rats.

Zhang L, Yuan B, Wang H, Gao Y - Biomed Res Int (2015)

Bottom Line: Administration of ABE dose-dependently reduced the elevated bilirubin level induced by phenylhydrazine.It can be somewhat supported from the results of in vitro bilirubin degradation experiment.ABE treatment also reduced the total antioxidant status (TAOS), cascade O2(-)/SOD, level of NF-κB protein, and adrenomedullin (AM).

View Article: PubMed Central - PubMed

Affiliation: Department of Pediatrics, Second Hospital of Xi'an Jiaotong University, Xi'an 710004, China.

ABSTRACT
The present study was designed to investigate the effect of Agaricus brasiliensis extract (ABE) on phenylhydrazine-induced neonatal jaundice in rats. Administration of ABE dose-dependently reduced the elevated bilirubin level induced by phenylhydrazine. It can be somewhat supported from the results of in vitro bilirubin degradation experiment. ABE treatment also reduced the total antioxidant status (TAOS), cascade O2(-)/SOD, level of NF-κB protein, and adrenomedullin (AM). Overall, the results of this study demonstrated that Agaricus brasiliensis extract may be beneficial to reducing bilirubin level without causing hepatotoxicity in neonatal jaundice.

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Histopathological analysis of rat liver sections using hematoxylin and eosin staining. (a) Section from a normal control rat liver. (b) Section from ABE-100 rat liver. (c) Section from ABE-50 rat liver. (d) Section from ABE-20 rat liver. (e) Section from a phenylhydrazine rat liver.
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fig2: Histopathological analysis of rat liver sections using hematoxylin and eosin staining. (a) Section from a normal control rat liver. (b) Section from ABE-100 rat liver. (c) Section from ABE-50 rat liver. (d) Section from ABE-20 rat liver. (e) Section from a phenylhydrazine rat liver.

Mentions: Phenylhydrazine induces neonatal jaundice conditions because it increases unconjugated bilirubin level without any significant change in the liver function. Liver function was evaluated by assessing serum ALT and AST, since AST and ALT are sensitive indicators of liver cell injury [23]. In the present study, we have confirmed that phenylhydrazine did not increase AST and ALT levels in serum of rats (Table 2). Consistent with the result, the liver histopathological observations also did not show any hepatic damage due to phenylhydrazine administration. As hypothesized, normal activity of the liver function enzymes and absence of any liver damage after ABE administration indicated the safety profile of ABE (Figure 2).


Therapeutic effect of Agaricus brasiliensis on phenylhydrazine-induced neonatal jaundice in rats.

Zhang L, Yuan B, Wang H, Gao Y - Biomed Res Int (2015)

Histopathological analysis of rat liver sections using hematoxylin and eosin staining. (a) Section from a normal control rat liver. (b) Section from ABE-100 rat liver. (c) Section from ABE-50 rat liver. (d) Section from ABE-20 rat liver. (e) Section from a phenylhydrazine rat liver.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4389989&req=5

fig2: Histopathological analysis of rat liver sections using hematoxylin and eosin staining. (a) Section from a normal control rat liver. (b) Section from ABE-100 rat liver. (c) Section from ABE-50 rat liver. (d) Section from ABE-20 rat liver. (e) Section from a phenylhydrazine rat liver.
Mentions: Phenylhydrazine induces neonatal jaundice conditions because it increases unconjugated bilirubin level without any significant change in the liver function. Liver function was evaluated by assessing serum ALT and AST, since AST and ALT are sensitive indicators of liver cell injury [23]. In the present study, we have confirmed that phenylhydrazine did not increase AST and ALT levels in serum of rats (Table 2). Consistent with the result, the liver histopathological observations also did not show any hepatic damage due to phenylhydrazine administration. As hypothesized, normal activity of the liver function enzymes and absence of any liver damage after ABE administration indicated the safety profile of ABE (Figure 2).

Bottom Line: Administration of ABE dose-dependently reduced the elevated bilirubin level induced by phenylhydrazine.It can be somewhat supported from the results of in vitro bilirubin degradation experiment.ABE treatment also reduced the total antioxidant status (TAOS), cascade O2(-)/SOD, level of NF-κB protein, and adrenomedullin (AM).

View Article: PubMed Central - PubMed

Affiliation: Department of Pediatrics, Second Hospital of Xi'an Jiaotong University, Xi'an 710004, China.

ABSTRACT
The present study was designed to investigate the effect of Agaricus brasiliensis extract (ABE) on phenylhydrazine-induced neonatal jaundice in rats. Administration of ABE dose-dependently reduced the elevated bilirubin level induced by phenylhydrazine. It can be somewhat supported from the results of in vitro bilirubin degradation experiment. ABE treatment also reduced the total antioxidant status (TAOS), cascade O2(-)/SOD, level of NF-κB protein, and adrenomedullin (AM). Overall, the results of this study demonstrated that Agaricus brasiliensis extract may be beneficial to reducing bilirubin level without causing hepatotoxicity in neonatal jaundice.

Show MeSH
Related in: MedlinePlus