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Diffuse calcifications protect carotid plaques regardless of the amount of neoangiogenesis and related histological complications.

Vasuri F, Fittipaldi S, Pini R, Degiovanni A, Mauro R, D'Errico-Grigioni A, Faggioli G, Stella A, Pasquinelli G - Biomed Res Int (2015)

Bottom Line: Previous data from our group showed that Nestin-positive intraplaque neovessels correlated with histological complications.Diffusely calcified plaques (13/73) were found predominantly in females (P = 0.017), with a significantly lower incidence of symptoms (TIA/stroke (P = 0.019) than noncalcified plaques but with the same incidence of histological complications (P = 0.156)).These results can be applied in a future presurgical identification of patients at major risk of developing symptoms.

View Article: PubMed Central - PubMed

Affiliation: Pathology Unit, Department of Experimental, Diagnostic and Specialty Medicine (DIMES), S. Orsola-Malpighi Hospital, Bologna University, Via Massarenti 9, 40138 Bologna, Italy.

ABSTRACT

Background: Neoangiogenesis is crucial in plaque progression and instability. Previous data from our group showed that Nestin-positive intraplaque neovessels correlated with histological complications. The aim of the present work is to evaluate the relationship between neoangiogenesis, plaque morphology, and clinical instability of the plaque.

Materials and methods: Seventy-three patients (53 males and 20 females, mean age 71 years) were consecutively enrolled. Clinical data and 14 histological variables, including intraplaque hemorrhage and calcifications, were collected. Immunohistochemistry for CD34 and Nestin was performed. RT-PCR was performed to evaluate Nestin mRNA (including 5 healthy arteries as controls).

Results: Diffusely calcified plaques (13/73) were found predominantly in females (P = 0.017), with a significantly lower incidence of symptoms (TIA/stroke (P = 0.019) than noncalcified plaques but with the same incidence of histological complications (P = 0.156)). Accordingly, calcified and noncalcified plaques showed similar mean densities of positivity for CD34 and Nestin. Nestin density, but not CD34, correlated with the occurrence of intraplaque hemorrhage.

Conclusions: Plaques with massive calcifications show the same incidence of histological complications but without influencing symptomatology, especially in female patients, and regardless of the amount of neoangiogenesis. These results can be applied in a future presurgical identification of patients at major risk of developing symptoms.

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Related in: MedlinePlus

Immunohistochemical staining for CD34 (a), (c), and (e) and Nestin (b), (d), and (f) in an uncomplicated noncalcified plaque (a) and (b), a calcified plaque (c) and (d), and a complicated noncalcified plaque (e) and (f). Neoangiogenesis in uncomplicated plaques is generally Nestin-negative. The overall neoangiogenesis in calcified plaques (complicated or not) is generally lower (both CD34 and Nestin) than in complicated plaques. Magnification 20x.
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fig2: Immunohistochemical staining for CD34 (a), (c), and (e) and Nestin (b), (d), and (f) in an uncomplicated noncalcified plaque (a) and (b), a calcified plaque (c) and (d), and a complicated noncalcified plaque (e) and (f). Neoangiogenesis in uncomplicated plaques is generally Nestin-negative. The overall neoangiogenesis in calcified plaques (complicated or not) is generally lower (both CD34 and Nestin) than in complicated plaques. Magnification 20x.

Mentions: The calcified plaques (including complicated and uncomplicated) showed overall less neoangiogenesis, measured with both CD34 and Nestin, than noncalcified plaques (P < 0.001, Table 3; Figure 2).


Diffuse calcifications protect carotid plaques regardless of the amount of neoangiogenesis and related histological complications.

Vasuri F, Fittipaldi S, Pini R, Degiovanni A, Mauro R, D'Errico-Grigioni A, Faggioli G, Stella A, Pasquinelli G - Biomed Res Int (2015)

Immunohistochemical staining for CD34 (a), (c), and (e) and Nestin (b), (d), and (f) in an uncomplicated noncalcified plaque (a) and (b), a calcified plaque (c) and (d), and a complicated noncalcified plaque (e) and (f). Neoangiogenesis in uncomplicated plaques is generally Nestin-negative. The overall neoangiogenesis in calcified plaques (complicated or not) is generally lower (both CD34 and Nestin) than in complicated plaques. Magnification 20x.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4389976&req=5

fig2: Immunohistochemical staining for CD34 (a), (c), and (e) and Nestin (b), (d), and (f) in an uncomplicated noncalcified plaque (a) and (b), a calcified plaque (c) and (d), and a complicated noncalcified plaque (e) and (f). Neoangiogenesis in uncomplicated plaques is generally Nestin-negative. The overall neoangiogenesis in calcified plaques (complicated or not) is generally lower (both CD34 and Nestin) than in complicated plaques. Magnification 20x.
Mentions: The calcified plaques (including complicated and uncomplicated) showed overall less neoangiogenesis, measured with both CD34 and Nestin, than noncalcified plaques (P < 0.001, Table 3; Figure 2).

Bottom Line: Previous data from our group showed that Nestin-positive intraplaque neovessels correlated with histological complications.Diffusely calcified plaques (13/73) were found predominantly in females (P = 0.017), with a significantly lower incidence of symptoms (TIA/stroke (P = 0.019) than noncalcified plaques but with the same incidence of histological complications (P = 0.156)).These results can be applied in a future presurgical identification of patients at major risk of developing symptoms.

View Article: PubMed Central - PubMed

Affiliation: Pathology Unit, Department of Experimental, Diagnostic and Specialty Medicine (DIMES), S. Orsola-Malpighi Hospital, Bologna University, Via Massarenti 9, 40138 Bologna, Italy.

ABSTRACT

Background: Neoangiogenesis is crucial in plaque progression and instability. Previous data from our group showed that Nestin-positive intraplaque neovessels correlated with histological complications. The aim of the present work is to evaluate the relationship between neoangiogenesis, plaque morphology, and clinical instability of the plaque.

Materials and methods: Seventy-three patients (53 males and 20 females, mean age 71 years) were consecutively enrolled. Clinical data and 14 histological variables, including intraplaque hemorrhage and calcifications, were collected. Immunohistochemistry for CD34 and Nestin was performed. RT-PCR was performed to evaluate Nestin mRNA (including 5 healthy arteries as controls).

Results: Diffusely calcified plaques (13/73) were found predominantly in females (P = 0.017), with a significantly lower incidence of symptoms (TIA/stroke (P = 0.019) than noncalcified plaques but with the same incidence of histological complications (P = 0.156)). Accordingly, calcified and noncalcified plaques showed similar mean densities of positivity for CD34 and Nestin. Nestin density, but not CD34, correlated with the occurrence of intraplaque hemorrhage.

Conclusions: Plaques with massive calcifications show the same incidence of histological complications but without influencing symptomatology, especially in female patients, and regardless of the amount of neoangiogenesis. These results can be applied in a future presurgical identification of patients at major risk of developing symptoms.

Show MeSH
Related in: MedlinePlus