Limits...
Attenuating tumour angiogenesis: a preventive role of metformin against breast cancer.

Gao S, Jiang J, Li P, Song H, Wang W, Li C, Kong D - Biomed Res Int (2015)

Bottom Line: No inhibition of breast tumour progression was detected.However, tumour necrosis was significantly increased in the metformin-treated group, accompanied by decreased capillary formation within the tumours.Thus, despite the lack of short-term benefit of metformin against tumour progression, a preventive role of metformin against breast cancer was implicated, which is at partially attributable to the attenuation of tumour angiogenesis.

View Article: PubMed Central - PubMed

Affiliation: Tianjin Key Laboratory of Biomaterial Research, Institute of Biomedical Engineering, Chinese Academy of Medical Science and Peking Union Medical College, Tianjin 300192, China.

ABSTRACT
Metformin is one of the most widely prescribed antidiabetics for type 2 diabetes. A critical role of metformin against tumorigenesis has recently been implicated, although several studies also reported the lack of anticancer property of the antidiabetics. Given the controversies regarding the potential role of metformin against tumour progression, the effect of metformin against breast, cervical, and ovarian tumour cell lines was examined followed by in vivo assessment of metformin on tumour growth using xenograft breast cancer models. Significant inhibitory impact of metformin was observed in MCF-7, HeLa, and SKOV-3 cells, suggesting an antiproliferative property of metformin against breast, cervical, and ovarian tumour cells, respectively, with the breast tumour cells, MCF-7, being the most responsive. In vivo assessment was subsequently carried out, where mice with breast tumours were treated with metformin (20 mg/kg body weight) or sterile PBS solution for 15 consecutive days. No inhibition of breast tumour progression was detected. However, tumour necrosis was significantly increased in the metformin-treated group, accompanied by decreased capillary formation within the tumours. Thus, despite the lack of short-term benefit of metformin against tumour progression, a preventive role of metformin against breast cancer was implicated, which is at partially attributable to the attenuation of tumour angiogenesis.

Show MeSH

Related in: MedlinePlus

Metformin inhibits tumour cell growth. Human ovarian (SKOV-3), breast (MCF-7), and cervical (HeLa) cells were exposed to a series of concentrations of metformin for 24 h ((a), (b), and (c)) and 5 days ((d), (e), and (f)). Cell viability was assessed using a cell viability (CCK-8) assay. Data are presented as means ± SD, n = 6.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4389975&req=5

fig1: Metformin inhibits tumour cell growth. Human ovarian (SKOV-3), breast (MCF-7), and cervical (HeLa) cells were exposed to a series of concentrations of metformin for 24 h ((a), (b), and (c)) and 5 days ((d), (e), and (f)). Cell viability was assessed using a cell viability (CCK-8) assay. Data are presented as means ± SD, n = 6.

Mentions: Given the high prevalence of ovarian, cervical, and, particularly, breast cancers in pre- and postmenopausal women, 3 female tumour cell lines, MCF-7, SKOV-3, and HeLa, were initially selected to investigate the potential anticancer effect of metformin in vitro. As shown in Figures 1(b) and 1(c), 24 h exposure to metformin significantly reduced cell viability in all 3 tumour cell lines, with a maximum response of 42 ± 8%, 38 ± 2%, 14 ± 2% for SKOV-3, MCF-7, and HeLa cells, respectively (Figures 1(a)–1(c)). Similar inhibitory responses were also observed from cells treated with metformin for 5 days (Figures 1(d)–1(f)). For both SKOV-3 and HeLa cells, the metformin-exerted attenuation of cell growth appeared to be concentration-dependent, in contrast to MCF-7, of which the inhibitory responses were similar once the administrative dose of metformin was over 20 mM. However, as noted by the National Cancer Institute some years ago, the activity of a pharmacological agent in vitro does not necessarily reflect its in vivo performance [8], and subsequent in vivo assessment was carried out.


Attenuating tumour angiogenesis: a preventive role of metformin against breast cancer.

Gao S, Jiang J, Li P, Song H, Wang W, Li C, Kong D - Biomed Res Int (2015)

Metformin inhibits tumour cell growth. Human ovarian (SKOV-3), breast (MCF-7), and cervical (HeLa) cells were exposed to a series of concentrations of metformin for 24 h ((a), (b), and (c)) and 5 days ((d), (e), and (f)). Cell viability was assessed using a cell viability (CCK-8) assay. Data are presented as means ± SD, n = 6.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4389975&req=5

fig1: Metformin inhibits tumour cell growth. Human ovarian (SKOV-3), breast (MCF-7), and cervical (HeLa) cells were exposed to a series of concentrations of metformin for 24 h ((a), (b), and (c)) and 5 days ((d), (e), and (f)). Cell viability was assessed using a cell viability (CCK-8) assay. Data are presented as means ± SD, n = 6.
Mentions: Given the high prevalence of ovarian, cervical, and, particularly, breast cancers in pre- and postmenopausal women, 3 female tumour cell lines, MCF-7, SKOV-3, and HeLa, were initially selected to investigate the potential anticancer effect of metformin in vitro. As shown in Figures 1(b) and 1(c), 24 h exposure to metformin significantly reduced cell viability in all 3 tumour cell lines, with a maximum response of 42 ± 8%, 38 ± 2%, 14 ± 2% for SKOV-3, MCF-7, and HeLa cells, respectively (Figures 1(a)–1(c)). Similar inhibitory responses were also observed from cells treated with metformin for 5 days (Figures 1(d)–1(f)). For both SKOV-3 and HeLa cells, the metformin-exerted attenuation of cell growth appeared to be concentration-dependent, in contrast to MCF-7, of which the inhibitory responses were similar once the administrative dose of metformin was over 20 mM. However, as noted by the National Cancer Institute some years ago, the activity of a pharmacological agent in vitro does not necessarily reflect its in vivo performance [8], and subsequent in vivo assessment was carried out.

Bottom Line: No inhibition of breast tumour progression was detected.However, tumour necrosis was significantly increased in the metformin-treated group, accompanied by decreased capillary formation within the tumours.Thus, despite the lack of short-term benefit of metformin against tumour progression, a preventive role of metformin against breast cancer was implicated, which is at partially attributable to the attenuation of tumour angiogenesis.

View Article: PubMed Central - PubMed

Affiliation: Tianjin Key Laboratory of Biomaterial Research, Institute of Biomedical Engineering, Chinese Academy of Medical Science and Peking Union Medical College, Tianjin 300192, China.

ABSTRACT
Metformin is one of the most widely prescribed antidiabetics for type 2 diabetes. A critical role of metformin against tumorigenesis has recently been implicated, although several studies also reported the lack of anticancer property of the antidiabetics. Given the controversies regarding the potential role of metformin against tumour progression, the effect of metformin against breast, cervical, and ovarian tumour cell lines was examined followed by in vivo assessment of metformin on tumour growth using xenograft breast cancer models. Significant inhibitory impact of metformin was observed in MCF-7, HeLa, and SKOV-3 cells, suggesting an antiproliferative property of metformin against breast, cervical, and ovarian tumour cells, respectively, with the breast tumour cells, MCF-7, being the most responsive. In vivo assessment was subsequently carried out, where mice with breast tumours were treated with metformin (20 mg/kg body weight) or sterile PBS solution for 15 consecutive days. No inhibition of breast tumour progression was detected. However, tumour necrosis was significantly increased in the metformin-treated group, accompanied by decreased capillary formation within the tumours. Thus, despite the lack of short-term benefit of metformin against tumour progression, a preventive role of metformin against breast cancer was implicated, which is at partially attributable to the attenuation of tumour angiogenesis.

Show MeSH
Related in: MedlinePlus