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Effect of mixotrophic growth on the ultrastructure and fatty acid composition of the diatom Synedra acus from Lake Baikal.

Shishlyannikov SM, Klimenkov IV, Bedoshvili YD, Mikhailov IS, Gorshkov AG - J Biol Res (Thessalon) (2014)

Bottom Line: Interest in studies concerning the effect of organic carbon sources on the growth of diatoms is largely aimed at subsequent physiological changes occurring in their cells.In this study, the results demonstrated that Synedra acus cells cultured in the presence of 80 mM glycerol contained lipid bodies of increased size, while cells from cultures supplemented with 40 mM glucose accumulated polysaccharide (chrysolaminarin) granules.Synedra acus subsp. radians have an ability to proliferate in the presence of high concentrations of organic substances and their transport into cells is evidence for its high adaptation potential, which, along with other factors, accounts for the dominance of this diatom in the spring-summer plankton of the oligotrophic Lake Baikal.

View Article: PubMed Central - PubMed

Affiliation: Limnological Institute, Siberian Branch, Russian Academy of Sciences, 3, Ulan-Batorskaya, St, P.O. Box 278, Irkutsk, 664033 Russia.

ABSTRACT

Background: Interest in studies concerning the effect of organic carbon sources on the growth of diatoms is largely aimed at subsequent physiological changes occurring in their cells. There are no data on structural changes in the cytoplasm and their relationship with changes in the composition of fatty acids in the course of mixotrophic culturing of freshwater diatoms. To elucidate the role of lipids in the growth of diatom cells in autotrophic and mixotrophic cultures, it is necessary to obtain information on the distribution of fatty acids among intracellular compartments and on possible ultrastructural changes in the cells.

Results: In this study, the results demonstrated that Synedra acus cells cultured in the presence of 80 mM glycerol contained lipid bodies of increased size, while cells from cultures supplemented with 40 mM glucose accumulated polysaccharide (chrysolaminarin) granules. An increase in the relative amounts of palmitic and stearic acids was revealed at the exponential growth phase of S. acus in the medium with 80 mM glycerol, which was indicative of the accumulation of fatty acids contained in triacylglycerols.

Conclusions: Synedra acus subsp. radians have an ability to proliferate in the presence of high concentrations of organic substances and their transport into cells is evidence for its high adaptation potential, which, along with other factors, accounts for the dominance of this diatom in the spring-summer plankton of the oligotrophic Lake Baikal.

No MeSH data available.


Dynamics ofS. acusabundance in autotrophic and mixotrophic cultures with different concentrations of glucose.
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Fig1: Dynamics ofS. acusabundance in autotrophic and mixotrophic cultures with different concentrations of glucose.

Mentions: In preliminary experiments, the maximum concentrations of organic carbon sources in the medium that did not result in a reduced rate of cell divisions or gradual cell death in S. acus culture were estimated at 40 mM for glucose and 80 mM for glycerol (Figures 1 and 2). Therefore, it is these concentrations that were used in subsequent mixotrophic cultures.Figure 1


Effect of mixotrophic growth on the ultrastructure and fatty acid composition of the diatom Synedra acus from Lake Baikal.

Shishlyannikov SM, Klimenkov IV, Bedoshvili YD, Mikhailov IS, Gorshkov AG - J Biol Res (Thessalon) (2014)

Dynamics ofS. acusabundance in autotrophic and mixotrophic cultures with different concentrations of glucose.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4389971&req=5

Fig1: Dynamics ofS. acusabundance in autotrophic and mixotrophic cultures with different concentrations of glucose.
Mentions: In preliminary experiments, the maximum concentrations of organic carbon sources in the medium that did not result in a reduced rate of cell divisions or gradual cell death in S. acus culture were estimated at 40 mM for glucose and 80 mM for glycerol (Figures 1 and 2). Therefore, it is these concentrations that were used in subsequent mixotrophic cultures.Figure 1

Bottom Line: Interest in studies concerning the effect of organic carbon sources on the growth of diatoms is largely aimed at subsequent physiological changes occurring in their cells.In this study, the results demonstrated that Synedra acus cells cultured in the presence of 80 mM glycerol contained lipid bodies of increased size, while cells from cultures supplemented with 40 mM glucose accumulated polysaccharide (chrysolaminarin) granules.Synedra acus subsp. radians have an ability to proliferate in the presence of high concentrations of organic substances and their transport into cells is evidence for its high adaptation potential, which, along with other factors, accounts for the dominance of this diatom in the spring-summer plankton of the oligotrophic Lake Baikal.

View Article: PubMed Central - PubMed

Affiliation: Limnological Institute, Siberian Branch, Russian Academy of Sciences, 3, Ulan-Batorskaya, St, P.O. Box 278, Irkutsk, 664033 Russia.

ABSTRACT

Background: Interest in studies concerning the effect of organic carbon sources on the growth of diatoms is largely aimed at subsequent physiological changes occurring in their cells. There are no data on structural changes in the cytoplasm and their relationship with changes in the composition of fatty acids in the course of mixotrophic culturing of freshwater diatoms. To elucidate the role of lipids in the growth of diatom cells in autotrophic and mixotrophic cultures, it is necessary to obtain information on the distribution of fatty acids among intracellular compartments and on possible ultrastructural changes in the cells.

Results: In this study, the results demonstrated that Synedra acus cells cultured in the presence of 80 mM glycerol contained lipid bodies of increased size, while cells from cultures supplemented with 40 mM glucose accumulated polysaccharide (chrysolaminarin) granules. An increase in the relative amounts of palmitic and stearic acids was revealed at the exponential growth phase of S. acus in the medium with 80 mM glycerol, which was indicative of the accumulation of fatty acids contained in triacylglycerols.

Conclusions: Synedra acus subsp. radians have an ability to proliferate in the presence of high concentrations of organic substances and their transport into cells is evidence for its high adaptation potential, which, along with other factors, accounts for the dominance of this diatom in the spring-summer plankton of the oligotrophic Lake Baikal.

No MeSH data available.