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A preliminary study of osteochondral regeneration using a scaffold-free three-dimensional construct of porcine adipose tissue-derived mesenchymal stem cells.

Murata D, Tokunaga S, Tamura T, Kawaguchi H, Miyoshi N, Fujiki M, Nakayama K, Misumi K - J Orthop Surg Res (2015)

Bottom Line: The histopathology of the implants after 6 months revealed active endochondral ossification underneath the plump fibrocartilage in animal no. 1.The histopathology after 12 months in animal no. 2 showed not only that the diminishing fibrocartilage was as thick as the surrounding normal cartilage but also that massive subchondral bone was present.The present results suggest that implantation of a scaffold-free 3D construct of AT-MSCs into an osteochondral defect may induce regeneration of the original structure of the cartilage and subchondral bone over the course of 1 year, although more experimental cases are needed.

View Article: PubMed Central - PubMed

Affiliation: Veterinary Surgery, Department of Veterinary Clinical Science, Joint Faculty of Veterinary Medicine, Kagoshima University, 21-24 Korimoto 1-chome, Kagoshima, 890-0065, Japan. daiki_net_offficial@yahoo.co.jp.

ABSTRACT

Background: Osteoarthritis (OA) is a major joint disease in humans and many other animals. Consequently, medical countermeasures for OA have been investigated diligently. This study was designed to examine the regeneration of articular cartilage and subchondral bone using three-dimensional (3D) constructs of adipose tissue-derived mesenchymal stem cells (AT-MSCs).

Methods: AT-MSCs were isolated and expanded until required for genetical and immunological analysis and construct creation. A construct consisting of about 760 spheroids that each contained 5.0 × 10(4) autologous AT-MSCs was implanted into an osteochondral defect (diameter: 4 mm; depth: 6 mm) created in the femoral trochlear groove of two adult microminipigs. After implantation, the defects were monitored by computed tomography every month for 6 months in animal no. 1 and 12 months in animal no. 2.

Results: AT-MSCs were confirmed to express the premature genes and to be positive for CD90 and CD105 and negative for CD34 and CD45. Under specific nutrient conditions, the AT-MSCs differentiated into osteogenic, chondrogenic, and adipogenic lineages, as evidenced by the expressions of related marker genes and the production of appropriate matrix molecules. A radiopaque area emerged from the boundary between the bone and the implant and increased more steadily upward and inward for the implants in both animal no. 1 and animal no. 2. The histopathology of the implants after 6 months revealed active endochondral ossification underneath the plump fibrocartilage in animal no. 1. The histopathology after 12 months in animal no. 2 showed not only that the diminishing fibrocartilage was as thick as the surrounding normal cartilage but also that massive subchondral bone was present.

Conclusions: The present results suggest that implantation of a scaffold-free 3D construct of AT-MSCs into an osteochondral defect may induce regeneration of the original structure of the cartilage and subchondral bone over the course of 1 year, although more experimental cases are needed.

No MeSH data available.


Related in: MedlinePlus

Macroscopic findings of the surface of the implanted and control sites. In animal no. 1, the surface of the implanted defect was covered with the abundant cartilaginous white tissues (A), whereas the cartilaginous tissue was scarce and the surface was depressed in the control site (B). In animal no. 2, the surface was more uniformly covered with abundant cartilaginous white tissues and the boundary to the surrounding normal cartilage was unclear in the implanted site (C), comparing to those of the control site (D).
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Fig6: Macroscopic findings of the surface of the implanted and control sites. In animal no. 1, the surface of the implanted defect was covered with the abundant cartilaginous white tissues (A), whereas the cartilaginous tissue was scarce and the surface was depressed in the control site (B). In animal no. 2, the surface was more uniformly covered with abundant cartilaginous white tissues and the boundary to the surrounding normal cartilage was unclear in the implanted site (C), comparing to those of the control site (D).

Mentions: Macroscopic examination of animal no. 1 revealed that the surface of the implanted defect was covered with abundant cartilaginous white tissue (Figure 6A), while cartilaginous tissue was scarce and the surface was depressed in the control site (Figure 6B). Similarly, in animal no. 2, the surface was quite uniformly covered with abundant cartilaginous white tissues and the boundary to the surrounding normal cartilage was unclear in the implanted site (Figure 6C), compared with the findings at the control site (Figure 6D). The average macroscopic scores for the implanted site were higher than those for the control site in animal no. 1, while the differences between the scores for the implanted site and the control site were decreased in animal no. 2 (Table 3).Figure 6


A preliminary study of osteochondral regeneration using a scaffold-free three-dimensional construct of porcine adipose tissue-derived mesenchymal stem cells.

Murata D, Tokunaga S, Tamura T, Kawaguchi H, Miyoshi N, Fujiki M, Nakayama K, Misumi K - J Orthop Surg Res (2015)

Macroscopic findings of the surface of the implanted and control sites. In animal no. 1, the surface of the implanted defect was covered with the abundant cartilaginous white tissues (A), whereas the cartilaginous tissue was scarce and the surface was depressed in the control site (B). In animal no. 2, the surface was more uniformly covered with abundant cartilaginous white tissues and the boundary to the surrounding normal cartilage was unclear in the implanted site (C), comparing to those of the control site (D).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4389925&req=5

Fig6: Macroscopic findings of the surface of the implanted and control sites. In animal no. 1, the surface of the implanted defect was covered with the abundant cartilaginous white tissues (A), whereas the cartilaginous tissue was scarce and the surface was depressed in the control site (B). In animal no. 2, the surface was more uniformly covered with abundant cartilaginous white tissues and the boundary to the surrounding normal cartilage was unclear in the implanted site (C), comparing to those of the control site (D).
Mentions: Macroscopic examination of animal no. 1 revealed that the surface of the implanted defect was covered with abundant cartilaginous white tissue (Figure 6A), while cartilaginous tissue was scarce and the surface was depressed in the control site (Figure 6B). Similarly, in animal no. 2, the surface was quite uniformly covered with abundant cartilaginous white tissues and the boundary to the surrounding normal cartilage was unclear in the implanted site (Figure 6C), compared with the findings at the control site (Figure 6D). The average macroscopic scores for the implanted site were higher than those for the control site in animal no. 1, while the differences between the scores for the implanted site and the control site were decreased in animal no. 2 (Table 3).Figure 6

Bottom Line: The histopathology of the implants after 6 months revealed active endochondral ossification underneath the plump fibrocartilage in animal no. 1.The histopathology after 12 months in animal no. 2 showed not only that the diminishing fibrocartilage was as thick as the surrounding normal cartilage but also that massive subchondral bone was present.The present results suggest that implantation of a scaffold-free 3D construct of AT-MSCs into an osteochondral defect may induce regeneration of the original structure of the cartilage and subchondral bone over the course of 1 year, although more experimental cases are needed.

View Article: PubMed Central - PubMed

Affiliation: Veterinary Surgery, Department of Veterinary Clinical Science, Joint Faculty of Veterinary Medicine, Kagoshima University, 21-24 Korimoto 1-chome, Kagoshima, 890-0065, Japan. daiki_net_offficial@yahoo.co.jp.

ABSTRACT

Background: Osteoarthritis (OA) is a major joint disease in humans and many other animals. Consequently, medical countermeasures for OA have been investigated diligently. This study was designed to examine the regeneration of articular cartilage and subchondral bone using three-dimensional (3D) constructs of adipose tissue-derived mesenchymal stem cells (AT-MSCs).

Methods: AT-MSCs were isolated and expanded until required for genetical and immunological analysis and construct creation. A construct consisting of about 760 spheroids that each contained 5.0 × 10(4) autologous AT-MSCs was implanted into an osteochondral defect (diameter: 4 mm; depth: 6 mm) created in the femoral trochlear groove of two adult microminipigs. After implantation, the defects were monitored by computed tomography every month for 6 months in animal no. 1 and 12 months in animal no. 2.

Results: AT-MSCs were confirmed to express the premature genes and to be positive for CD90 and CD105 and negative for CD34 and CD45. Under specific nutrient conditions, the AT-MSCs differentiated into osteogenic, chondrogenic, and adipogenic lineages, as evidenced by the expressions of related marker genes and the production of appropriate matrix molecules. A radiopaque area emerged from the boundary between the bone and the implant and increased more steadily upward and inward for the implants in both animal no. 1 and animal no. 2. The histopathology of the implants after 6 months revealed active endochondral ossification underneath the plump fibrocartilage in animal no. 1. The histopathology after 12 months in animal no. 2 showed not only that the diminishing fibrocartilage was as thick as the surrounding normal cartilage but also that massive subchondral bone was present.

Conclusions: The present results suggest that implantation of a scaffold-free 3D construct of AT-MSCs into an osteochondral defect may induce regeneration of the original structure of the cartilage and subchondral bone over the course of 1 year, although more experimental cases are needed.

No MeSH data available.


Related in: MedlinePlus