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A "schizophotonic" all-in-one nanoparticle coating for multiplexed SE(R)RS biomedical imaging.

Iacono P, Karabeber H, Kircher MF - Angew. Chem. Int. Ed. Engl. (2014)

Bottom Line: Traditional synthetic routes require high metal-dye affinities and are challenged by unfavorable electrostatic interactions and limited scalability.The integration of various SERS reporters into a biocompatible polymeric surface coating allows for controlled dye incorporation, high colloidal stability, and optimized in vivo circulation times.We demonstrate the capability of this all-in-one gold surface coating and SERS reporter for multiplexed lymph-node imaging.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiology and Center for Molecular Imaging and Nanotechnology (CMINT), Memorial Sloan Kettering Cancer Center and Weill Cornell Medical College, New York, NY 10065 (USA).

No MeSH data available.


Related in: MedlinePlus

a) Composition of a SERS-active gold nanoparticle featuring an all-inclusive near-IR-active surface coating SERS substrate. b) Transmission electron microscopy (TEM) image of Au@IR-pHPMA.
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fig01: a) Composition of a SERS-active gold nanoparticle featuring an all-inclusive near-IR-active surface coating SERS substrate. b) Transmission electron microscopy (TEM) image of Au@IR-pHPMA.

Mentions: As an alternative to silicate layers, hydrophilic polymers as SERS substrate surface coatings are appealing because they can minimize nanoparticle size while retaining excellent biocompatibility and long intravascular circulation times desirable for efficient disease targeting.[5b] The polymers can also be tailored to be functionalized after they have been grafted onto the metal surface, allowing for subsequent particle modification. The facile syntheses of these SERS nanoprobes generally include treating dye-adhered metal nanoparticles with thiolated poly(ethylene glycol) or analogous polymers. However, competition for vacant binding sites can limit signal intensities and/or polymer grafting densities.[5b, 9] Therefore, a hydrophilic NIR-active polymer with the potential for further modification would be an ideal substrate for SERS applications, allowing for an all-encompassing surface coating for gold nanoparticles, retaining optimal (multi-)chromophore loading efficiency, minimizing size, and retaining its biocompatibility, water solubility, and circulative properties. Herein we report a class of hydrophilic NIR dye-loaded poly(N-(2-hydroxypropyl) methacrylamides) (pHPMA; Figure 1) and their application for gold nanoparticle-based SERS imaging of lymph nodes.


A "schizophotonic" all-in-one nanoparticle coating for multiplexed SE(R)RS biomedical imaging.

Iacono P, Karabeber H, Kircher MF - Angew. Chem. Int. Ed. Engl. (2014)

a) Composition of a SERS-active gold nanoparticle featuring an all-inclusive near-IR-active surface coating SERS substrate. b) Transmission electron microscopy (TEM) image of Au@IR-pHPMA.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4389888&req=5

fig01: a) Composition of a SERS-active gold nanoparticle featuring an all-inclusive near-IR-active surface coating SERS substrate. b) Transmission electron microscopy (TEM) image of Au@IR-pHPMA.
Mentions: As an alternative to silicate layers, hydrophilic polymers as SERS substrate surface coatings are appealing because they can minimize nanoparticle size while retaining excellent biocompatibility and long intravascular circulation times desirable for efficient disease targeting.[5b] The polymers can also be tailored to be functionalized after they have been grafted onto the metal surface, allowing for subsequent particle modification. The facile syntheses of these SERS nanoprobes generally include treating dye-adhered metal nanoparticles with thiolated poly(ethylene glycol) or analogous polymers. However, competition for vacant binding sites can limit signal intensities and/or polymer grafting densities.[5b, 9] Therefore, a hydrophilic NIR-active polymer with the potential for further modification would be an ideal substrate for SERS applications, allowing for an all-encompassing surface coating for gold nanoparticles, retaining optimal (multi-)chromophore loading efficiency, minimizing size, and retaining its biocompatibility, water solubility, and circulative properties. Herein we report a class of hydrophilic NIR dye-loaded poly(N-(2-hydroxypropyl) methacrylamides) (pHPMA; Figure 1) and their application for gold nanoparticle-based SERS imaging of lymph nodes.

Bottom Line: Traditional synthetic routes require high metal-dye affinities and are challenged by unfavorable electrostatic interactions and limited scalability.The integration of various SERS reporters into a biocompatible polymeric surface coating allows for controlled dye incorporation, high colloidal stability, and optimized in vivo circulation times.We demonstrate the capability of this all-in-one gold surface coating and SERS reporter for multiplexed lymph-node imaging.

View Article: PubMed Central - PubMed

Affiliation: Department of Radiology and Center for Molecular Imaging and Nanotechnology (CMINT), Memorial Sloan Kettering Cancer Center and Weill Cornell Medical College, New York, NY 10065 (USA).

No MeSH data available.


Related in: MedlinePlus