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A photochromic sensor microchip for high-performance multiplex metal ions detection.

Huang Y, Li F, Ye C, Qin M, Ran W, Song Y - Sci Rep (2015)

Bottom Line: It is expected to develop new principles and techniques to achieve high-performance multi-analytes testing with facile sensors.The multi-testing sensor array performed in dark, ultraviolet or visual stimulation, corresponding to different molecular states of spirooxazine metal ions coordination.The facile photochromic microchip contributes a multi-states array sensing method, and will open new opportunities for the development of advanced discriminant analysis for complex analytes.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Green Printing, Institute of Chemistry, Chinese Academy of Sciences.

ABSTRACT
Current multi-analytes chips are limited with requiring numbers of sensors, complex synthesis and compounds screen. It is expected to develop new principles and techniques to achieve high-performance multi-analytes testing with facile sensors. Here, we investigated the correlative multi-states properties of a photochromic sensor (spirooxazine), which is capable of a selective and cross-reactive sensor array for discriminated multi-analytes (11 metal ions) detection by just one sensing compound. The multi-testing sensor array performed in dark, ultraviolet or visual stimulation, corresponding to different molecular states of spirooxazine metal ions coordination. The facile photochromic microchip contributes a multi-states array sensing method, and will open new opportunities for the development of advanced discriminant analysis for complex analytes.

No MeSH data available.


(a) Fluorescent image and (b) 3D representation of the integrated fluorescence intensity of the photochromic SP microchip constitute from rows from each light irradiations process of the photochromic microchip. The Fluorescent image of photochromic microchip spotted by metal ions (1.0 mM in water, 200 nL), which was recorded in 6 different channels. The colours were generated by superimposing of the equally weighed images corresponding to RGB channels.
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f4: (a) Fluorescent image and (b) 3D representation of the integrated fluorescence intensity of the photochromic SP microchip constitute from rows from each light irradiations process of the photochromic microchip. The Fluorescent image of photochromic microchip spotted by metal ions (1.0 mM in water, 200 nL), which was recorded in 6 different channels. The colours were generated by superimposing of the equally weighed images corresponding to RGB channels.

Mentions: Based on the fluorescent enhancement or shift of different spirooxazine-metallic coordination states, we designed a microchip adopting spirooxazine as the only one sensor, analysing the fluorescent signals to realize multiple metal ions detection. To generate the cross-reactive SP microchip, we carried out 1.0 mM 1, 3, 3-trimethylindolinonaphthospirooxazine and thermoplastic polyurethane Tecoflex® (0.5% PU in Ethanol) solution (200 nL) pipetted onto each pixel of the microchip. Here we used PU as the interface medium for the sensing in each pixel. PU is a block polymer with terephthalic acid units and ethylene glycol units, which is solvable in ethanol and swellable in water. On this microchip, AlCl3, CaCl2, CdCl2, CoCl2, CrCl3, CuCl2, FeCl2, HgCl2, MgCl2, NiCl2 and ZnCl2 (1.0 mM in water, 200 nL, pH = 5) and pH = 5 control aqueous solutions (HCl-NaCl) were spotted on corresponding pixels in 12 rows respectively. The fluorescence responses of the photochromic spirooxazine microchip to the presence of 11 metal ions (Al3+, Ca2+, Cd2+, Co2+, Cr3+, Cu2+, Fe2+, Hg2+, Mg2+, Ni2+ and Zn2+) were recorded in 6 channels (CH 1: 450 nm, CH 2: 480 nm, CH 3: 505 nm, CH 4: 535 nm, CH 5: 570 nm and CH 6: 605 nm, with UV 365 nm excitation). The colours were generated by superimposing of the equally weighed images corresponding to RGB channels. Dark, UV and Vis stimulation resulted in the spirooxazine microchip displaying various fluorescent signals. For better comparing the fluorescent difference of dark, UV and Vis process, rows from each light irradiation steps were selected to constitute Fig. 4. The fluorescent image in Fig. 4b displays obvious different fluorescence intensity and shift for each row, which will contribute more information for high-performance multi-testing.


A photochromic sensor microchip for high-performance multiplex metal ions detection.

Huang Y, Li F, Ye C, Qin M, Ran W, Song Y - Sci Rep (2015)

(a) Fluorescent image and (b) 3D representation of the integrated fluorescence intensity of the photochromic SP microchip constitute from rows from each light irradiations process of the photochromic microchip. The Fluorescent image of photochromic microchip spotted by metal ions (1.0 mM in water, 200 nL), which was recorded in 6 different channels. The colours were generated by superimposing of the equally weighed images corresponding to RGB channels.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4389811&req=5

f4: (a) Fluorescent image and (b) 3D representation of the integrated fluorescence intensity of the photochromic SP microchip constitute from rows from each light irradiations process of the photochromic microchip. The Fluorescent image of photochromic microchip spotted by metal ions (1.0 mM in water, 200 nL), which was recorded in 6 different channels. The colours were generated by superimposing of the equally weighed images corresponding to RGB channels.
Mentions: Based on the fluorescent enhancement or shift of different spirooxazine-metallic coordination states, we designed a microchip adopting spirooxazine as the only one sensor, analysing the fluorescent signals to realize multiple metal ions detection. To generate the cross-reactive SP microchip, we carried out 1.0 mM 1, 3, 3-trimethylindolinonaphthospirooxazine and thermoplastic polyurethane Tecoflex® (0.5% PU in Ethanol) solution (200 nL) pipetted onto each pixel of the microchip. Here we used PU as the interface medium for the sensing in each pixel. PU is a block polymer with terephthalic acid units and ethylene glycol units, which is solvable in ethanol and swellable in water. On this microchip, AlCl3, CaCl2, CdCl2, CoCl2, CrCl3, CuCl2, FeCl2, HgCl2, MgCl2, NiCl2 and ZnCl2 (1.0 mM in water, 200 nL, pH = 5) and pH = 5 control aqueous solutions (HCl-NaCl) were spotted on corresponding pixels in 12 rows respectively. The fluorescence responses of the photochromic spirooxazine microchip to the presence of 11 metal ions (Al3+, Ca2+, Cd2+, Co2+, Cr3+, Cu2+, Fe2+, Hg2+, Mg2+, Ni2+ and Zn2+) were recorded in 6 channels (CH 1: 450 nm, CH 2: 480 nm, CH 3: 505 nm, CH 4: 535 nm, CH 5: 570 nm and CH 6: 605 nm, with UV 365 nm excitation). The colours were generated by superimposing of the equally weighed images corresponding to RGB channels. Dark, UV and Vis stimulation resulted in the spirooxazine microchip displaying various fluorescent signals. For better comparing the fluorescent difference of dark, UV and Vis process, rows from each light irradiation steps were selected to constitute Fig. 4. The fluorescent image in Fig. 4b displays obvious different fluorescence intensity and shift for each row, which will contribute more information for high-performance multi-testing.

Bottom Line: It is expected to develop new principles and techniques to achieve high-performance multi-analytes testing with facile sensors.The multi-testing sensor array performed in dark, ultraviolet or visual stimulation, corresponding to different molecular states of spirooxazine metal ions coordination.The facile photochromic microchip contributes a multi-states array sensing method, and will open new opportunities for the development of advanced discriminant analysis for complex analytes.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Green Printing, Institute of Chemistry, Chinese Academy of Sciences.

ABSTRACT
Current multi-analytes chips are limited with requiring numbers of sensors, complex synthesis and compounds screen. It is expected to develop new principles and techniques to achieve high-performance multi-analytes testing with facile sensors. Here, we investigated the correlative multi-states properties of a photochromic sensor (spirooxazine), which is capable of a selective and cross-reactive sensor array for discriminated multi-analytes (11 metal ions) detection by just one sensing compound. The multi-testing sensor array performed in dark, ultraviolet or visual stimulation, corresponding to different molecular states of spirooxazine metal ions coordination. The facile photochromic microchip contributes a multi-states array sensing method, and will open new opportunities for the development of advanced discriminant analysis for complex analytes.

No MeSH data available.