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Antioxidant effect of a polyphenol-rich grape pomace extract on motility, viability and lipid peroxidation of thawed bovine spermatozoa.

Sapanidou VG, Margaritis I, Siahos N, Arsenopoulos K, Dragatidou E, Taitzoglou IA, Zervos IA, Theodoridis A, Tsantarliotou MP - J Biol Res (Thessalon) (2014)

Bottom Line: The other parameters did not show statistically significant changes.Moreover, the presence of 2 μg ml(-1) and 5 μg ml(-1) of the extract kept the levels of Malondialdehyde (MDA) production in significantly lower level, compared to the other groups, after 2 hrs and 4 hrs of incubation (p <0.05).Particularly, a dose-dependent effect was noticed after 2 hrs of incubation.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Physiology, School of Veterinary Medicine, Faculty of Health Science, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece.

ABSTRACT

Background: Grape extracts of the Greek species Vitis vinifera possess potent antioxidant properties in vitro. The freeze/thaw process and the preparation of semen during assisted reproductive techniques can adversely affect the functional integrity of spermatozoa. The objective was to assess the effect of three different concentrations (1 μg ml(-1), 2 μg ml(-1) and 5 μg ml(-1)) of a polyphenol-rich grape pomace extract on motility, viability, acrosomal and lipid peroxidation status of thawed bovine spermatozoa after 2 and 4 hrs of incubation.

Results: The results indicate that the percentage of "Rapid" spermatozoa remained significantly increased (p <0.05) in the presence of 5 μg ml(-1) of the extract, compared to the control after 2 hrs of incubation. Additionally, the incubation of spermatozoa with 2 μg ml(-1) and 5 μg ml(-1) of the extract for 2 hrs resulted in a significantly better maintenance of viable spermatozoa with intact acrosome (p <0.05). The other parameters did not show statistically significant changes. Moreover, the presence of 2 μg ml(-1) and 5 μg ml(-1) of the extract kept the levels of Malondialdehyde (MDA) production in significantly lower level, compared to the other groups, after 2 hrs and 4 hrs of incubation (p <0.05). Particularly, a dose-dependent effect was noticed after 2 hrs of incubation.

Conclusions: Our results suggest that the grape pomace extract exerts a powerful antioxidant role, by suppressing lipid peroxidation, and provides protection in terms of motility and acrosomal integrity, which are correlated with in vivo fertility. The optimal extract concentration is 5 μg ml(-1).

No MeSH data available.


Related in: MedlinePlus

The effect of the grape extract on LPO production of sperm (mean ± SD). Different letters indicate statistically significant differences between different concentrations within each time point (p <0.05).
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Fig1: The effect of the grape extract on LPO production of sperm (mean ± SD). Different letters indicate statistically significant differences between different concentrations within each time point (p <0.05).

Mentions: Figure 1 summarizes the results of the TBARS assay. Corresponding to supplementation, all the groups treated with the grape pomace extract had lower MDA production compared to the control, significantly or not. During 2 hrs of incubation, a dose-dependent reduction in MDA levels was observed. Moreover, statistically significant differences were noticed between: i) control and 2 μg ml−1 (p = 0.002), ii) control and 5 μg ml−1 (p <0.001), iii) 1 μg ml−1 and 5 μg ml−1 (p = 0.001) and iv) 5 μg ml−1 and all groups (p <0.03). After 4 hrs of incubation, the maximum antioxidant effect was observed in the presence of 2 μg ml−1 compared to all the other groups, while 5 μg ml−1 decreased the production of MDA significantly, compared to the control group (p <0.05). Nevertheless, no statistically significant difference was observed between 2 μg ml−1 and 5 μg ml−1 after 4 hrs of incubation (p >0.05).Figure 1


Antioxidant effect of a polyphenol-rich grape pomace extract on motility, viability and lipid peroxidation of thawed bovine spermatozoa.

Sapanidou VG, Margaritis I, Siahos N, Arsenopoulos K, Dragatidou E, Taitzoglou IA, Zervos IA, Theodoridis A, Tsantarliotou MP - J Biol Res (Thessalon) (2014)

The effect of the grape extract on LPO production of sperm (mean ± SD). Different letters indicate statistically significant differences between different concentrations within each time point (p <0.05).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4389799&req=5

Fig1: The effect of the grape extract on LPO production of sperm (mean ± SD). Different letters indicate statistically significant differences between different concentrations within each time point (p <0.05).
Mentions: Figure 1 summarizes the results of the TBARS assay. Corresponding to supplementation, all the groups treated with the grape pomace extract had lower MDA production compared to the control, significantly or not. During 2 hrs of incubation, a dose-dependent reduction in MDA levels was observed. Moreover, statistically significant differences were noticed between: i) control and 2 μg ml−1 (p = 0.002), ii) control and 5 μg ml−1 (p <0.001), iii) 1 μg ml−1 and 5 μg ml−1 (p = 0.001) and iv) 5 μg ml−1 and all groups (p <0.03). After 4 hrs of incubation, the maximum antioxidant effect was observed in the presence of 2 μg ml−1 compared to all the other groups, while 5 μg ml−1 decreased the production of MDA significantly, compared to the control group (p <0.05). Nevertheless, no statistically significant difference was observed between 2 μg ml−1 and 5 μg ml−1 after 4 hrs of incubation (p >0.05).Figure 1

Bottom Line: The other parameters did not show statistically significant changes.Moreover, the presence of 2 μg ml(-1) and 5 μg ml(-1) of the extract kept the levels of Malondialdehyde (MDA) production in significantly lower level, compared to the other groups, after 2 hrs and 4 hrs of incubation (p <0.05).Particularly, a dose-dependent effect was noticed after 2 hrs of incubation.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Physiology, School of Veterinary Medicine, Faculty of Health Science, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece.

ABSTRACT

Background: Grape extracts of the Greek species Vitis vinifera possess potent antioxidant properties in vitro. The freeze/thaw process and the preparation of semen during assisted reproductive techniques can adversely affect the functional integrity of spermatozoa. The objective was to assess the effect of three different concentrations (1 μg ml(-1), 2 μg ml(-1) and 5 μg ml(-1)) of a polyphenol-rich grape pomace extract on motility, viability, acrosomal and lipid peroxidation status of thawed bovine spermatozoa after 2 and 4 hrs of incubation.

Results: The results indicate that the percentage of "Rapid" spermatozoa remained significantly increased (p <0.05) in the presence of 5 μg ml(-1) of the extract, compared to the control after 2 hrs of incubation. Additionally, the incubation of spermatozoa with 2 μg ml(-1) and 5 μg ml(-1) of the extract for 2 hrs resulted in a significantly better maintenance of viable spermatozoa with intact acrosome (p <0.05). The other parameters did not show statistically significant changes. Moreover, the presence of 2 μg ml(-1) and 5 μg ml(-1) of the extract kept the levels of Malondialdehyde (MDA) production in significantly lower level, compared to the other groups, after 2 hrs and 4 hrs of incubation (p <0.05). Particularly, a dose-dependent effect was noticed after 2 hrs of incubation.

Conclusions: Our results suggest that the grape pomace extract exerts a powerful antioxidant role, by suppressing lipid peroxidation, and provides protection in terms of motility and acrosomal integrity, which are correlated with in vivo fertility. The optimal extract concentration is 5 μg ml(-1).

No MeSH data available.


Related in: MedlinePlus