Limits...
Use of the comet assay technique for quick and reliable prediction of in vitro response to chemotherapeutics in breast and colon cancer.

Apostolou P, Toloudi M, Kourtidou E, Mimikakou G, Vlachou I, Chatziioannou M, Papasotiriou I - J Biol Res (Thessalon) (2014)

Bottom Line: Determination of response to chemotherapy is a major requirement of personalized medicine.Resistance, whether developed or native, critically affects a treatment's success.The comet assay technique can provide reliable and quick results with minimum requirements and is applicable to a wide variety of drugs.

View Article: PubMed Central - PubMed

Affiliation: Research Genetic Cancer Centre Ltd (R.G.C.C. Ltd), Filotas, Florina Greece.

ABSTRACT

Background: Determination of response to chemotherapy is a major requirement of personalized medicine. Resistance, whether developed or native, critically affects a treatment's success. Single Cell Gel lectrophoresis - also known as a comet assay - is used to detect DNA damage at the level of individual eukaryotic cells. We assessed the use of comet assays in determining response to chemotherapeutic drugs that are widely used in breast and colon cancer.

Results: We treated human breast and colon cancer cell lines with melphalan, cisplatin, mechlorethamine or doxorubicin, as monotherapies. Drug activities varied even in the same cancer types, further demonstrating the heterogeneity of different cancer types.

Conclusion: The comet assay technique can provide reliable and quick results with minimum requirements and is applicable to a wide variety of drugs.

No MeSH data available.


Related in: MedlinePlus

Presentation of comet assay data by using a five-grade scale. The comets are classified according to extent of DNA in tail and a value 0-4 is given (0: almost no DNA in tail, 4: almost the entire DNA in tail). By measuring 100 comets, an overall score between 0 and 400 arbitrary units is calculated. A: Treatment with cisplatin in colorectal cancer cell lines. B: Treatment with melphalan in breast cancer cell lines C: Treatment with mechlorethamine in breast cancer cell lines D: Treatment with doxorubicin in breast cancer cell lines.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC4389674&req=5

Fig4: Presentation of comet assay data by using a five-grade scale. The comets are classified according to extent of DNA in tail and a value 0-4 is given (0: almost no DNA in tail, 4: almost the entire DNA in tail). By measuring 100 comets, an overall score between 0 and 400 arbitrary units is calculated. A: Treatment with cisplatin in colorectal cancer cell lines. B: Treatment with melphalan in breast cancer cell lines C: Treatment with mechlorethamine in breast cancer cell lines D: Treatment with doxorubicin in breast cancer cell lines.

Mentions: Comet visual scoring can be also calculated based on a five-grade scale from 0 to 4. In a grade 4 comet cell, the entire DNA is in the “tail”, while in grade 0 the entire DNA is in “head”. These values given to comets can be summarized and provide a quantitative measure for 100 cells on a scale from 0-400. These data are presented in Figure 4. A high score means that the cells have been damaged, and the most DNA is in “tail”. The decrease of the score indicates unfragmented cells, therefore a positive effect by the particular drug. The higher the decrease, the greater the effect of the chemotherapeutic. As it is presented in Figure 4, it is displayed little difference in the score of T47D under the treatment with mechlorethamine. It is also observed that among the colorectal cancer cell lines, cisplatin affects HCT-15 more than the others. Among MCF-7, MDA-MB 231 and T47D, the MCF-7 cell line is more affected by melphalan and doxorubicin, while MDA-MB 231 is more sensitive in mechlorethamine.Figure 4


Use of the comet assay technique for quick and reliable prediction of in vitro response to chemotherapeutics in breast and colon cancer.

Apostolou P, Toloudi M, Kourtidou E, Mimikakou G, Vlachou I, Chatziioannou M, Papasotiriou I - J Biol Res (Thessalon) (2014)

Presentation of comet assay data by using a five-grade scale. The comets are classified according to extent of DNA in tail and a value 0-4 is given (0: almost no DNA in tail, 4: almost the entire DNA in tail). By measuring 100 comets, an overall score between 0 and 400 arbitrary units is calculated. A: Treatment with cisplatin in colorectal cancer cell lines. B: Treatment with melphalan in breast cancer cell lines C: Treatment with mechlorethamine in breast cancer cell lines D: Treatment with doxorubicin in breast cancer cell lines.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4389674&req=5

Fig4: Presentation of comet assay data by using a five-grade scale. The comets are classified according to extent of DNA in tail and a value 0-4 is given (0: almost no DNA in tail, 4: almost the entire DNA in tail). By measuring 100 comets, an overall score between 0 and 400 arbitrary units is calculated. A: Treatment with cisplatin in colorectal cancer cell lines. B: Treatment with melphalan in breast cancer cell lines C: Treatment with mechlorethamine in breast cancer cell lines D: Treatment with doxorubicin in breast cancer cell lines.
Mentions: Comet visual scoring can be also calculated based on a five-grade scale from 0 to 4. In a grade 4 comet cell, the entire DNA is in the “tail”, while in grade 0 the entire DNA is in “head”. These values given to comets can be summarized and provide a quantitative measure for 100 cells on a scale from 0-400. These data are presented in Figure 4. A high score means that the cells have been damaged, and the most DNA is in “tail”. The decrease of the score indicates unfragmented cells, therefore a positive effect by the particular drug. The higher the decrease, the greater the effect of the chemotherapeutic. As it is presented in Figure 4, it is displayed little difference in the score of T47D under the treatment with mechlorethamine. It is also observed that among the colorectal cancer cell lines, cisplatin affects HCT-15 more than the others. Among MCF-7, MDA-MB 231 and T47D, the MCF-7 cell line is more affected by melphalan and doxorubicin, while MDA-MB 231 is more sensitive in mechlorethamine.Figure 4

Bottom Line: Determination of response to chemotherapy is a major requirement of personalized medicine.Resistance, whether developed or native, critically affects a treatment's success.The comet assay technique can provide reliable and quick results with minimum requirements and is applicable to a wide variety of drugs.

View Article: PubMed Central - PubMed

Affiliation: Research Genetic Cancer Centre Ltd (R.G.C.C. Ltd), Filotas, Florina Greece.

ABSTRACT

Background: Determination of response to chemotherapy is a major requirement of personalized medicine. Resistance, whether developed or native, critically affects a treatment's success. Single Cell Gel lectrophoresis - also known as a comet assay - is used to detect DNA damage at the level of individual eukaryotic cells. We assessed the use of comet assays in determining response to chemotherapeutic drugs that are widely used in breast and colon cancer.

Results: We treated human breast and colon cancer cell lines with melphalan, cisplatin, mechlorethamine or doxorubicin, as monotherapies. Drug activities varied even in the same cancer types, further demonstrating the heterogeneity of different cancer types.

Conclusion: The comet assay technique can provide reliable and quick results with minimum requirements and is applicable to a wide variety of drugs.

No MeSH data available.


Related in: MedlinePlus