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Epidemiology, antibiotic consumption and molecular characterisation of Staphylococcus aureus infections--data from the Polish Neonatology Surveillance Network, 2009-2012.

Romaniszyn D, Różańska A, Wójkowska-Mach J, Chmielarczyk A, Pobiega M, Adamski P, Helwich E, Lauterbach R, Borszewska-Kornacka M, Gulczyńska E, Kordek A, Bulanda M - BMC Infect. Dis. (2015)

Bottom Line: MRSA-infections were diagnosed significantly earlier than MSSA-infections in these newborns (14th day vs. 23rd day (p=0.0194)).One dominant clone was found among the 14 MRSA isolates.Results obtained in the study point at specific epidemiological situation in Polish NICU (more detailed studies are recommended).

View Article: PubMed Central - PubMed

Affiliation: Chair of Microbiology, Jagiellonian University Medical College, 18 Czysta Street, Kracow, 31-121, Poland. d.romaniszyn@uj.edu.pl.

ABSTRACT

Background: Our aim was to determine and characterize S. aureus (SA) isolated from infections in newborns for antibiotic resistance, virulence factors, genotypes, epidemiology and antibiotic consumption.

Methods: Prospective surveillance of infections was conducted. Data about antibiotic treatment were analyzed. Antimicrobial susceptibility was assessed. PCR amplification was used to detect resistance and virulence genes. Typing methods such as PFGE, spa-typing and SCCmec were used.

Results: SA was found to be associated with 6.5% of infections. Methicillin-Resistant Staphylococcus aureus accounted for 32.8% of SA-infections. An incidence of MRSA-infections was 1.1/1000 newborns. MRSA-infections were diagnosed significantly earlier than MSSA-infections in these newborns (14th day vs. 23rd day (p=0.0194)). MRSA-infections increased the risk of newborn's death. Antibiotic consumption in both group was similar, but a high level of glycopeptides-usage for MSSA infections was observed. In the MRSA group, more strains were resistant to erythromycin, clindamycin, gentamicin and amikacin than in the MSSA group. Hla gene was present in 93.9% of strains, and seg and sei in 65.3% of strains, respectively. One dominant clone was found among the 14 MRSA isolates. Fifteen strains belonging to SCCmec type IV were spa-t015 and one strain belonging to SCCmec type V was spa-t011.

Conclusions: Results obtained in the study point at specific epidemiological situation in Polish NICU (more detailed studies are recommended). High usage of glycopeptides in the MSSA infections treatment indicates the necessity of antimicrobial stewardship improvement and introducing molecular screening for early identification of infections.

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Results of PFGE, SCCmecandspatyping ofS.aureusisolates.
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Fig1: Results of PFGE, SCCmecandspatyping ofS.aureusisolates.

Mentions: MSSA isolates showed very different pulsotypes; dominant clones were not detected. Cluster analysis based on PFGE of the 33 isolates showed 23 pulsotypes, some of which were less than 70% similar, suggesting a genotypically variable population. Isolates with identical pulsotypes were usually derived from different patients in the same NICU during the same period of time. Analysis of SmaI macro-restriction profiles of the 16 MRSA isolates revealed one dominant clone (14 isolates). The two remaining MRSA isolates had different PFGE patterns (Figure 1). MRSA isolates belonging to the dominant clone were derived from one NICU designed by the letter F. Two spa types and two SCCmec were observed: spa-t015 in the case of 15 isolates belonging to the SCCmec type IV and spa-t011 in one isolate belonging to the SCCmec type V.Figure 1


Epidemiology, antibiotic consumption and molecular characterisation of Staphylococcus aureus infections--data from the Polish Neonatology Surveillance Network, 2009-2012.

Romaniszyn D, Różańska A, Wójkowska-Mach J, Chmielarczyk A, Pobiega M, Adamski P, Helwich E, Lauterbach R, Borszewska-Kornacka M, Gulczyńska E, Kordek A, Bulanda M - BMC Infect. Dis. (2015)

Results of PFGE, SCCmecandspatyping ofS.aureusisolates.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4389670&req=5

Fig1: Results of PFGE, SCCmecandspatyping ofS.aureusisolates.
Mentions: MSSA isolates showed very different pulsotypes; dominant clones were not detected. Cluster analysis based on PFGE of the 33 isolates showed 23 pulsotypes, some of which were less than 70% similar, suggesting a genotypically variable population. Isolates with identical pulsotypes were usually derived from different patients in the same NICU during the same period of time. Analysis of SmaI macro-restriction profiles of the 16 MRSA isolates revealed one dominant clone (14 isolates). The two remaining MRSA isolates had different PFGE patterns (Figure 1). MRSA isolates belonging to the dominant clone were derived from one NICU designed by the letter F. Two spa types and two SCCmec were observed: spa-t015 in the case of 15 isolates belonging to the SCCmec type IV and spa-t011 in one isolate belonging to the SCCmec type V.Figure 1

Bottom Line: MRSA-infections were diagnosed significantly earlier than MSSA-infections in these newborns (14th day vs. 23rd day (p=0.0194)).One dominant clone was found among the 14 MRSA isolates.Results obtained in the study point at specific epidemiological situation in Polish NICU (more detailed studies are recommended).

View Article: PubMed Central - PubMed

Affiliation: Chair of Microbiology, Jagiellonian University Medical College, 18 Czysta Street, Kracow, 31-121, Poland. d.romaniszyn@uj.edu.pl.

ABSTRACT

Background: Our aim was to determine and characterize S. aureus (SA) isolated from infections in newborns for antibiotic resistance, virulence factors, genotypes, epidemiology and antibiotic consumption.

Methods: Prospective surveillance of infections was conducted. Data about antibiotic treatment were analyzed. Antimicrobial susceptibility was assessed. PCR amplification was used to detect resistance and virulence genes. Typing methods such as PFGE, spa-typing and SCCmec were used.

Results: SA was found to be associated with 6.5% of infections. Methicillin-Resistant Staphylococcus aureus accounted for 32.8% of SA-infections. An incidence of MRSA-infections was 1.1/1000 newborns. MRSA-infections were diagnosed significantly earlier than MSSA-infections in these newborns (14th day vs. 23rd day (p=0.0194)). MRSA-infections increased the risk of newborn's death. Antibiotic consumption in both group was similar, but a high level of glycopeptides-usage for MSSA infections was observed. In the MRSA group, more strains were resistant to erythromycin, clindamycin, gentamicin and amikacin than in the MSSA group. Hla gene was present in 93.9% of strains, and seg and sei in 65.3% of strains, respectively. One dominant clone was found among the 14 MRSA isolates. Fifteen strains belonging to SCCmec type IV were spa-t015 and one strain belonging to SCCmec type V was spa-t011.

Conclusions: Results obtained in the study point at specific epidemiological situation in Polish NICU (more detailed studies are recommended). High usage of glycopeptides in the MSSA infections treatment indicates the necessity of antimicrobial stewardship improvement and introducing molecular screening for early identification of infections.

Show MeSH
Related in: MedlinePlus