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Relationship of vitamin D status and bone mass according to vitamin D-binding protein genotypes.

Nimitphong H, Sritara C, Chailurkit LO, Chanprasertyothin S, Ratanachaiwong W, Sritara P, Ongphiphadhanakul B - Nutr J (2015)

Bottom Line: But a trend of positive correlation was observed for the DBP genotypes with total hip BMD, and for the interaction between 25(OH)D and DBP genotypes with BMD at all femoral sites.We further analyzed data according to DBP genotypes.Differences in DBP genotypes additionally influenced the correlation of fetuin-A levels with femoral BMD.

View Article: PubMed Central - PubMed

Affiliation: Department of Medicine and Research Center, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Rama VI Road, Ratchathewi, Bangkok, 10400, Thailand. hataikarnn@hotmail.com.

ABSTRACT

Background: Vitamin D-binding protein (DBP) may alter the biological activity of total 25-hydroxyvitamin D [25(OH)D]; this could influence on the effects of vitamin D in relation to bone mineral density (BMD) and fractures. Emerging data suggest that fetuin-A may be involved in bone metabolism. We aimed to investigate the influence of DBP gene polymorphism on the relationship of vitamin D status and fetuin-A levels to BMD and bone markers.

Methods: This cross-sectional study was part of a health survey of employees of the Electricity Generating Authority of Thailand (1,734 healthy subjects, 72% male). Fasting blood samples were assayed for 25(OH)D, fetuin-A, N-terminal propeptides of type 1 procollagen (P1NP), C-terminal cross-linking telopeptides of type I collagen (CTx-I), and DBP rs2282679 genotypes. L1-L4 lumbar spine and femoral BMD were measured using dual-energy X-ray absorptiometry.

Results: The DBP rs2282679 genotype distribution conformed to the Hardy-Weinberg equilibrium. There were no correlations between 25(OH)D levels and BMD and bone markers. But a trend of positive correlation was observed for the DBP genotypes with total hip BMD, and for the interaction between 25(OH)D and DBP genotypes with BMD at all femoral sites. We further analyzed data according to DBP genotypes. Only in subjects with the AA (common) genotype, 25(OH)D levels were positively related to BMD and bone markers, while fetuin-A was negatively related to total hip BMD, independently of age, gender and BMI.

Conclusions: The interaction between vitamin D status, as measured by circulating 25(OH)D and DBP rs2282679 genotypes, modified the association between 25(OH)D and BMD and bone markers. Differences in DBP genotypes additionally influenced the correlation of fetuin-A levels with femoral BMD.

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Related in: MedlinePlus

25(OH)D levels of eachDBPrs2282679 genotype in total subject, male and female. The square box represented mean ± SD. The upper and lower bar represented the upper and lower values. a: p < 0.05, different from CC group. b: p < 0.05, different from CA group.
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Fig2: 25(OH)D levels of eachDBPrs2282679 genotype in total subject, male and female. The square box represented mean ± SD. The upper and lower bar represented the upper and lower values. a: p < 0.05, different from CC group. b: p < 0.05, different from CA group.

Mentions: The DBP rs2282679 genotype distribution conformed to the Hardy–Weinberg equilibrium: i.e. AA (999; 57.6%), CA (637; 36.7%), and CC (98; 5.7%). Other than 25(OH)D levels, there were no differences in clinical characteristics between subjects in each DBP genotype of the entire cohort (combine males and females; Table 2) or in subgroup of males or females (data not showed). For vitamin D status, subjects with the AA genotype had the highest 25(OH)D levels (64.6 ± 15.5 nmol/L) compared with those in the CA and CC groups (59.2 ± 14.2 and 53.0 ± 10.6 nmol/L, respectively; p <0.001, Table 1). For each DBP genotype, 25(OH)D levels in males were higher than in females, as presented in Figure 1. 25(OH)D levels in each DBP genotype were all significantly different in males (Figure 2). In female, the difference in 25(OH)D levels were found only between those in CC vs. AA and CA vs. AA genotype (Figure 2).Figure 1


Relationship of vitamin D status and bone mass according to vitamin D-binding protein genotypes.

Nimitphong H, Sritara C, Chailurkit LO, Chanprasertyothin S, Ratanachaiwong W, Sritara P, Ongphiphadhanakul B - Nutr J (2015)

25(OH)D levels of eachDBPrs2282679 genotype in total subject, male and female. The square box represented mean ± SD. The upper and lower bar represented the upper and lower values. a: p < 0.05, different from CC group. b: p < 0.05, different from CA group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4389666&req=5

Fig2: 25(OH)D levels of eachDBPrs2282679 genotype in total subject, male and female. The square box represented mean ± SD. The upper and lower bar represented the upper and lower values. a: p < 0.05, different from CC group. b: p < 0.05, different from CA group.
Mentions: The DBP rs2282679 genotype distribution conformed to the Hardy–Weinberg equilibrium: i.e. AA (999; 57.6%), CA (637; 36.7%), and CC (98; 5.7%). Other than 25(OH)D levels, there were no differences in clinical characteristics between subjects in each DBP genotype of the entire cohort (combine males and females; Table 2) or in subgroup of males or females (data not showed). For vitamin D status, subjects with the AA genotype had the highest 25(OH)D levels (64.6 ± 15.5 nmol/L) compared with those in the CA and CC groups (59.2 ± 14.2 and 53.0 ± 10.6 nmol/L, respectively; p <0.001, Table 1). For each DBP genotype, 25(OH)D levels in males were higher than in females, as presented in Figure 1. 25(OH)D levels in each DBP genotype were all significantly different in males (Figure 2). In female, the difference in 25(OH)D levels were found only between those in CC vs. AA and CA vs. AA genotype (Figure 2).Figure 1

Bottom Line: But a trend of positive correlation was observed for the DBP genotypes with total hip BMD, and for the interaction between 25(OH)D and DBP genotypes with BMD at all femoral sites.We further analyzed data according to DBP genotypes.Differences in DBP genotypes additionally influenced the correlation of fetuin-A levels with femoral BMD.

View Article: PubMed Central - PubMed

Affiliation: Department of Medicine and Research Center, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Rama VI Road, Ratchathewi, Bangkok, 10400, Thailand. hataikarnn@hotmail.com.

ABSTRACT

Background: Vitamin D-binding protein (DBP) may alter the biological activity of total 25-hydroxyvitamin D [25(OH)D]; this could influence on the effects of vitamin D in relation to bone mineral density (BMD) and fractures. Emerging data suggest that fetuin-A may be involved in bone metabolism. We aimed to investigate the influence of DBP gene polymorphism on the relationship of vitamin D status and fetuin-A levels to BMD and bone markers.

Methods: This cross-sectional study was part of a health survey of employees of the Electricity Generating Authority of Thailand (1,734 healthy subjects, 72% male). Fasting blood samples were assayed for 25(OH)D, fetuin-A, N-terminal propeptides of type 1 procollagen (P1NP), C-terminal cross-linking telopeptides of type I collagen (CTx-I), and DBP rs2282679 genotypes. L1-L4 lumbar spine and femoral BMD were measured using dual-energy X-ray absorptiometry.

Results: The DBP rs2282679 genotype distribution conformed to the Hardy-Weinberg equilibrium. There were no correlations between 25(OH)D levels and BMD and bone markers. But a trend of positive correlation was observed for the DBP genotypes with total hip BMD, and for the interaction between 25(OH)D and DBP genotypes with BMD at all femoral sites. We further analyzed data according to DBP genotypes. Only in subjects with the AA (common) genotype, 25(OH)D levels were positively related to BMD and bone markers, while fetuin-A was negatively related to total hip BMD, independently of age, gender and BMI.

Conclusions: The interaction between vitamin D status, as measured by circulating 25(OH)D and DBP rs2282679 genotypes, modified the association between 25(OH)D and BMD and bone markers. Differences in DBP genotypes additionally influenced the correlation of fetuin-A levels with femoral BMD.

Show MeSH
Related in: MedlinePlus