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Prosomeric organization of the hypothalamus in an elasmobranch, the catshark Scyliorhinus canicula.

Santos-Durán GN, Menuet A, Lagadec R, Mayeur H, Ferreiro-Galve S, Mazan S, Rodríguez-Moldes I, Candal E - Front Neuroanat (2015)

Bottom Line: Deciphering its embryonic and adult organization is crucial in an evolutionary approach of the organization of the vertebrate forebrain.Analysis of the results within the updated prosomeric model framework support the existence of alar and basal histogenetic compartments in the hypothalamus similar to those described in the mouse, suggesting the ancestrality of these subdivisions in jawed vertebrates.These data provide new insights into hypothalamic organization in cartilaginous fishes and highlight the generality of key features of the prosomeric model in jawed vertebrates.

View Article: PubMed Central - PubMed

Affiliation: Centro de Investigaciones Biológicas, Department of Cell Biology and Ecology, University of Santiago de Compostela Santiago de Compostela, Spain.

ABSTRACT
The hypothalamus has been a central topic in neuroanatomy because of its important physiological functions, but its mature organization remains elusive. Deciphering its embryonic and adult organization is crucial in an evolutionary approach of the organization of the vertebrate forebrain. Here we studied the molecular organization of the hypothalamus and neighboring telencephalic domains in a cartilaginous fish, the catshark, Scyliorhinus canicula, focusing on ScFoxg1a, ScShh, ScNkx2.1, ScDlx2/5, ScOtp, and ScTbr1 expression profiles and on the identification α-acetylated-tubulin-immunoreactive (ir), TH-ir, 5-HT-ir, and GFAP-ir structures by means of immunohistochemistry. Analysis of the results within the updated prosomeric model framework support the existence of alar and basal histogenetic compartments in the hypothalamus similar to those described in the mouse, suggesting the ancestrality of these subdivisions in jawed vertebrates. These data provide new insights into hypothalamic organization in cartilaginous fishes and highlight the generality of key features of the prosomeric model in jawed vertebrates.

No MeSH data available.


Loss of ScNkx2.1 early expression following cyclopamine treatment. Lateral views of the cephalic region of stage 18 catshark embryos following whole-mount in situ hybridization (ISH) with the ScNkx2.1 probe. Control (A) and cyclopamine-treated (B) embryos as described in Section “Materials and Methods” are shown. The number (n) of embryos used is indicated in each case. The signal is lost in the latter. Scale bars: 500 μm.
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Figure 5: Loss of ScNkx2.1 early expression following cyclopamine treatment. Lateral views of the cephalic region of stage 18 catshark embryos following whole-mount in situ hybridization (ISH) with the ScNkx2.1 probe. Control (A) and cyclopamine-treated (B) embryos as described in Section “Materials and Methods” are shown. The number (n) of embryos used is indicated in each case. The signal is lost in the latter. Scale bars: 500 μm.

Mentions: In order to test whether, as in osteichthyans, the initiation of ScNkx2.1 expression in the forebrain is dependent on Shh, we used in ovo injections of the Shh inhibitor cyclopamine. All control embryos (n = 4) exhibited the expected ScNkx2.1 signal in the rostral-most and ventral-most portion of the forebrain (Figure 5). This signal was lost in all embryos dissected following cyclopamine treatment (n = 3), supporting the conclusion that Shh signaling is required for the initiation of ScNkx2.1 expression in S. canicula as in osteichthyans.


Prosomeric organization of the hypothalamus in an elasmobranch, the catshark Scyliorhinus canicula.

Santos-Durán GN, Menuet A, Lagadec R, Mayeur H, Ferreiro-Galve S, Mazan S, Rodríguez-Moldes I, Candal E - Front Neuroanat (2015)

Loss of ScNkx2.1 early expression following cyclopamine treatment. Lateral views of the cephalic region of stage 18 catshark embryos following whole-mount in situ hybridization (ISH) with the ScNkx2.1 probe. Control (A) and cyclopamine-treated (B) embryos as described in Section “Materials and Methods” are shown. The number (n) of embryos used is indicated in each case. The signal is lost in the latter. Scale bars: 500 μm.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4389657&req=5

Figure 5: Loss of ScNkx2.1 early expression following cyclopamine treatment. Lateral views of the cephalic region of stage 18 catshark embryos following whole-mount in situ hybridization (ISH) with the ScNkx2.1 probe. Control (A) and cyclopamine-treated (B) embryos as described in Section “Materials and Methods” are shown. The number (n) of embryos used is indicated in each case. The signal is lost in the latter. Scale bars: 500 μm.
Mentions: In order to test whether, as in osteichthyans, the initiation of ScNkx2.1 expression in the forebrain is dependent on Shh, we used in ovo injections of the Shh inhibitor cyclopamine. All control embryos (n = 4) exhibited the expected ScNkx2.1 signal in the rostral-most and ventral-most portion of the forebrain (Figure 5). This signal was lost in all embryos dissected following cyclopamine treatment (n = 3), supporting the conclusion that Shh signaling is required for the initiation of ScNkx2.1 expression in S. canicula as in osteichthyans.

Bottom Line: Deciphering its embryonic and adult organization is crucial in an evolutionary approach of the organization of the vertebrate forebrain.Analysis of the results within the updated prosomeric model framework support the existence of alar and basal histogenetic compartments in the hypothalamus similar to those described in the mouse, suggesting the ancestrality of these subdivisions in jawed vertebrates.These data provide new insights into hypothalamic organization in cartilaginous fishes and highlight the generality of key features of the prosomeric model in jawed vertebrates.

View Article: PubMed Central - PubMed

Affiliation: Centro de Investigaciones Biológicas, Department of Cell Biology and Ecology, University of Santiago de Compostela Santiago de Compostela, Spain.

ABSTRACT
The hypothalamus has been a central topic in neuroanatomy because of its important physiological functions, but its mature organization remains elusive. Deciphering its embryonic and adult organization is crucial in an evolutionary approach of the organization of the vertebrate forebrain. Here we studied the molecular organization of the hypothalamus and neighboring telencephalic domains in a cartilaginous fish, the catshark, Scyliorhinus canicula, focusing on ScFoxg1a, ScShh, ScNkx2.1, ScDlx2/5, ScOtp, and ScTbr1 expression profiles and on the identification α-acetylated-tubulin-immunoreactive (ir), TH-ir, 5-HT-ir, and GFAP-ir structures by means of immunohistochemistry. Analysis of the results within the updated prosomeric model framework support the existence of alar and basal histogenetic compartments in the hypothalamus similar to those described in the mouse, suggesting the ancestrality of these subdivisions in jawed vertebrates. These data provide new insights into hypothalamic organization in cartilaginous fishes and highlight the generality of key features of the prosomeric model in jawed vertebrates.

No MeSH data available.