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Comparison of praziquantel pharmacokinetics and tissue distribution in fresh and brackish water cultured grass carp (ctenopharyngodon idellus) after oral administration of single bolus.

Xie X, Zhao Y, Yang X, Hu K - BMC Vet. Res. (2015)

Bottom Line: Praziquantel (PZQ) is an effective pesticide against monogeneans.According to the One-Way ANOVA, the results showed that the salinity had a significant effect on the drug concentration of plasma (p < 0.01), muscle (p < 0.01), liver (p < 0.01) and kidney (p < 0.01) in the all sampling time points between the brackish water grass carps and the freshwater grass carps, wherein, PZQ plasma and tissue concentrations in the brackish water group were constantly lower than that in the freshwater group.The elimination half-lives (t1/2β) in plasma and all tissues of the freshwater group were significantly longer than that in the brackish water group.

View Article: PubMed Central - PubMed

Affiliation: College of Aquaculture and Life Science, Shanghai Ocean University, No. 999, Huchenghuan Road, Shanghai, 200090, P.R. China. 284451765@qq.com.

ABSTRACT

Background: Praziquantel (PZQ) is an effective pesticide against monogeneans. Its pharmacokinetics in fish may be affected by water environment and temperature. The present study was designed to compare the pharmacokinetics, tissue distribution, and elimination of PZQ in freshwater-acclimated grass carp and brackish water cultured grass carp. Plasma and tissue PZQ concentrations were determined after a single 10 mg/kg oral PZQ dose.

Results: The datas of plasma and tissues drug concentration was calculated by the software SPSS 13.0. According to the One-Way ANOVA, the results showed that the salinity had a significant effect on the drug concentration of plasma (p < 0.01), muscle (p < 0.01), liver (p < 0.01) and kidney (p < 0.01) in the all sampling time points between the brackish water grass carps and the freshwater grass carps, wherein, PZQ plasma and tissue concentrations in the brackish water group were constantly lower than that in the freshwater group. The peak PZQ levels of plasma, muscle, liver, and kidneys in the brackish water group were 0.76 μg/ml, 0.51 μg/g, 2.7 μg/g, and 2.99 μg/g, respectively; and that in the freshwater group were 0.91 μg/ml, 0.62 μg/g, 3.87 μg/g, and 3.39 μg/g, respectively. The elimination half-lives (t1/2β) in plasma and all tissues of the freshwater group were significantly longer than that in the brackish water group. The elimination half-lives (t1/2β) of plasma, muscle, liver and kidneys in brackish water grass carps were 56.46, 36.17, 15.31, and 132.64 h, respectively; and that in the freshwater grass carps were 71.15, 44.88, 23.86, and 150.23 h, respectively.

Conclusion: These findings indicate that water environment affects the tissue distribution and elimination of PZQ in grass carps, the elimination in brackish water grass carps is more rapid than that in fresh water grass carps and tissue concentrations of brackish water grass carps are lower than that in freshwater grass carps after orally administrating the same dosage at the same water temperature. We speculate that the main excretion pathway of the drug is through renal elimination, and the decreased kidney function in brackish water grass carps is likely responsible for the considerable difference in pharmacokinetics between the two groups of grass carps.

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Praziquantel in plasma and tissues of freshwater and brackish water grass carps after oral administration. Standard deviation is given at vertical bars. (n = 5, The mean ± SD). (A: plasma; B: muscle; C: liver; D: kidney).
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Fig1: Praziquantel in plasma and tissues of freshwater and brackish water grass carps after oral administration. Standard deviation is given at vertical bars. (n = 5, The mean ± SD). (A: plasma; B: muscle; C: liver; D: kidney).

Mentions: The PZQ concentration-time curves are shown in Figure 1. The pharmacokinetic parameters are shown in Table 1. The plasma, muscle and liver of freshwater group grass carp and the plasma, muscle and kidney of brackish water group PZQ concentration-time profiles were best described by a two-compartmental open pharmacokinetic model with first-order absorption. The kidney of freshwater group and liver of brackish water group PZQ concentration-time profiles were best described by a three-compartmental open pharmacokinetic model. The datas of plasma and tissues drug concentration was calculated by the software SPSS 13.0. According to the One-Way ANOVA, the results showed that the salinity had a significant effect on the drug concentration of plasma (p < 0.01), musle (p < 0.01), liver (p < 0.01) and kidney (p < 0.01) in the all sampling time points between the brackish water grass carps and the freshwater grass carps.Figure 1


Comparison of praziquantel pharmacokinetics and tissue distribution in fresh and brackish water cultured grass carp (ctenopharyngodon idellus) after oral administration of single bolus.

Xie X, Zhao Y, Yang X, Hu K - BMC Vet. Res. (2015)

Praziquantel in plasma and tissues of freshwater and brackish water grass carps after oral administration. Standard deviation is given at vertical bars. (n = 5, The mean ± SD). (A: plasma; B: muscle; C: liver; D: kidney).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4389578&req=5

Fig1: Praziquantel in plasma and tissues of freshwater and brackish water grass carps after oral administration. Standard deviation is given at vertical bars. (n = 5, The mean ± SD). (A: plasma; B: muscle; C: liver; D: kidney).
Mentions: The PZQ concentration-time curves are shown in Figure 1. The pharmacokinetic parameters are shown in Table 1. The plasma, muscle and liver of freshwater group grass carp and the plasma, muscle and kidney of brackish water group PZQ concentration-time profiles were best described by a two-compartmental open pharmacokinetic model with first-order absorption. The kidney of freshwater group and liver of brackish water group PZQ concentration-time profiles were best described by a three-compartmental open pharmacokinetic model. The datas of plasma and tissues drug concentration was calculated by the software SPSS 13.0. According to the One-Way ANOVA, the results showed that the salinity had a significant effect on the drug concentration of plasma (p < 0.01), musle (p < 0.01), liver (p < 0.01) and kidney (p < 0.01) in the all sampling time points between the brackish water grass carps and the freshwater grass carps.Figure 1

Bottom Line: Praziquantel (PZQ) is an effective pesticide against monogeneans.According to the One-Way ANOVA, the results showed that the salinity had a significant effect on the drug concentration of plasma (p < 0.01), muscle (p < 0.01), liver (p < 0.01) and kidney (p < 0.01) in the all sampling time points between the brackish water grass carps and the freshwater grass carps, wherein, PZQ plasma and tissue concentrations in the brackish water group were constantly lower than that in the freshwater group.The elimination half-lives (t1/2β) in plasma and all tissues of the freshwater group were significantly longer than that in the brackish water group.

View Article: PubMed Central - PubMed

Affiliation: College of Aquaculture and Life Science, Shanghai Ocean University, No. 999, Huchenghuan Road, Shanghai, 200090, P.R. China. 284451765@qq.com.

ABSTRACT

Background: Praziquantel (PZQ) is an effective pesticide against monogeneans. Its pharmacokinetics in fish may be affected by water environment and temperature. The present study was designed to compare the pharmacokinetics, tissue distribution, and elimination of PZQ in freshwater-acclimated grass carp and brackish water cultured grass carp. Plasma and tissue PZQ concentrations were determined after a single 10 mg/kg oral PZQ dose.

Results: The datas of plasma and tissues drug concentration was calculated by the software SPSS 13.0. According to the One-Way ANOVA, the results showed that the salinity had a significant effect on the drug concentration of plasma (p < 0.01), muscle (p < 0.01), liver (p < 0.01) and kidney (p < 0.01) in the all sampling time points between the brackish water grass carps and the freshwater grass carps, wherein, PZQ plasma and tissue concentrations in the brackish water group were constantly lower than that in the freshwater group. The peak PZQ levels of plasma, muscle, liver, and kidneys in the brackish water group were 0.76 μg/ml, 0.51 μg/g, 2.7 μg/g, and 2.99 μg/g, respectively; and that in the freshwater group were 0.91 μg/ml, 0.62 μg/g, 3.87 μg/g, and 3.39 μg/g, respectively. The elimination half-lives (t1/2β) in plasma and all tissues of the freshwater group were significantly longer than that in the brackish water group. The elimination half-lives (t1/2β) of plasma, muscle, liver and kidneys in brackish water grass carps were 56.46, 36.17, 15.31, and 132.64 h, respectively; and that in the freshwater grass carps were 71.15, 44.88, 23.86, and 150.23 h, respectively.

Conclusion: These findings indicate that water environment affects the tissue distribution and elimination of PZQ in grass carps, the elimination in brackish water grass carps is more rapid than that in fresh water grass carps and tissue concentrations of brackish water grass carps are lower than that in freshwater grass carps after orally administrating the same dosage at the same water temperature. We speculate that the main excretion pathway of the drug is through renal elimination, and the decreased kidney function in brackish water grass carps is likely responsible for the considerable difference in pharmacokinetics between the two groups of grass carps.

Show MeSH
Related in: MedlinePlus