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Long-term infusion of nesfatin-1 causes a sustained regulation of whole-body energy homeostasis of male Fischer 344 rats.

Mortazavi S, Gonzalez R, Ceddia R, Unniappan S - Front Cell Dev Biol (2015)

Bottom Line: Nesfatin-1, the N-terminal fragment of nucleobindin 2 (NUCB2), is an 82 amino-acid peptide that inhibits food intake and exerts weight-reducing effects.On the seventh day of nesfatin-1 infusion, cumulative food intake, and total spontaneous physical activity during the dark phase were significantly reduced and elevated, respectively.Collectively, our results indicate that chronic peripheral administration of nesfatin-1 at the dose tested, results in a sustained reduction in food intake and modulation of whole body energy homeostasis.

View Article: PubMed Central - PubMed

Affiliation: Department of Veterinary Biomedical Sciences, Western College of Veterinary Medicine, University of Saskatchewan Saskatoon, SK, Canada.

ABSTRACT
Nesfatin-1, the N-terminal fragment of nucleobindin 2 (NUCB2), is an 82 amino-acid peptide that inhibits food intake and exerts weight-reducing effects. Nesfatin-1 has been proposed as a potential anti-obesity peptide. However, studies to date have mainly focused on the acute satiety effects of centrally administered nesfatin-1. The main objective of our studies was to characterize the long-term/chronic effects of peripheral administration of nesfatin-1 on whole-body energy balance and metabolic partitioning in male Fischer 344 rats. Short-term (1 day) subcutaneous infusion of nesfatin-1 (50 μg/kg body weight/day) using osmotic mini-pumps increased spontaneous physical activity and whole-body fat oxidation during the dark phase. This was accompanied by decreased food intake and basal metabolic rate compared to saline infused controls. On the seventh day of nesfatin-1 infusion, cumulative food intake, and total spontaneous physical activity during the dark phase were significantly reduced and elevated, respectively. Meanwhile, intraperitoneal injection of nesfatin-1 only caused a dark phase specific reduction in food intake and an increase in physical activity. NUCB2 mRNA expression in the brain and stomach, as well as serum NUCB2 concentrations were significantly reduced after 24 h fasting, while a post-prandial increase in serum NUCB2 was found in ad libitum fed rats. Collectively, our results indicate that chronic peripheral administration of nesfatin-1 at the dose tested, results in a sustained reduction in food intake and modulation of whole body energy homeostasis.

No MeSH data available.


Related in: MedlinePlus

Fasting for 18 h reduces NUCB2 mRNA expression in the brain and stomach (A) of rats. Re-feeding for 4 h normalized NUCB2 mRNA expression in the brain (A). Serum NUCB2 levels were lower in rats fasted for 18 h (B). There was a post-prandial increase in serum nesfatin-1/NUCB2 levels in rats 3 h after the beginning of dark phase (C). All data are presented as means ± SEM. n = samples from six rats analyzed in duplicates. *P < 0.05 compared to fed rats or at 6 p.m., **P < 0.01 compared to unfed rats.
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Figure 7: Fasting for 18 h reduces NUCB2 mRNA expression in the brain and stomach (A) of rats. Re-feeding for 4 h normalized NUCB2 mRNA expression in the brain (A). Serum NUCB2 levels were lower in rats fasted for 18 h (B). There was a post-prandial increase in serum nesfatin-1/NUCB2 levels in rats 3 h after the beginning of dark phase (C). All data are presented as means ± SEM. n = samples from six rats analyzed in duplicates. *P < 0.05 compared to fed rats or at 6 p.m., **P < 0.01 compared to unfed rats.

Mentions: Compared to the expression levels in ad libitum fed controls, NUCB2 mRNA expression in the brain and stomach was significantly reduced (~60%) after food deprivation for 24 h (Figure 7A). Re-feeding for 3 h after the 24-h food deprivation resulted in a return of brain NUCB2 mRNA expression to that in the brain of fed rats (Figure 7A). Serum nesfatin-1/NUCB2 levels in rats deprived of food for 24 h were significantly lower compared to that in ad libitum fed rats (Figure 7B). Levels of circulating nesfatin-1/NUCB2 significantly increased upon feeding (at 2200 h; 3 h after commencing the dark phase), compared to that found at a time immediately prior to the dark phase (at 1800 h; final hour of the light phase) (Figure 7C).


Long-term infusion of nesfatin-1 causes a sustained regulation of whole-body energy homeostasis of male Fischer 344 rats.

Mortazavi S, Gonzalez R, Ceddia R, Unniappan S - Front Cell Dev Biol (2015)

Fasting for 18 h reduces NUCB2 mRNA expression in the brain and stomach (A) of rats. Re-feeding for 4 h normalized NUCB2 mRNA expression in the brain (A). Serum NUCB2 levels were lower in rats fasted for 18 h (B). There was a post-prandial increase in serum nesfatin-1/NUCB2 levels in rats 3 h after the beginning of dark phase (C). All data are presented as means ± SEM. n = samples from six rats analyzed in duplicates. *P < 0.05 compared to fed rats or at 6 p.m., **P < 0.01 compared to unfed rats.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4389570&req=5

Figure 7: Fasting for 18 h reduces NUCB2 mRNA expression in the brain and stomach (A) of rats. Re-feeding for 4 h normalized NUCB2 mRNA expression in the brain (A). Serum NUCB2 levels were lower in rats fasted for 18 h (B). There was a post-prandial increase in serum nesfatin-1/NUCB2 levels in rats 3 h after the beginning of dark phase (C). All data are presented as means ± SEM. n = samples from six rats analyzed in duplicates. *P < 0.05 compared to fed rats or at 6 p.m., **P < 0.01 compared to unfed rats.
Mentions: Compared to the expression levels in ad libitum fed controls, NUCB2 mRNA expression in the brain and stomach was significantly reduced (~60%) after food deprivation for 24 h (Figure 7A). Re-feeding for 3 h after the 24-h food deprivation resulted in a return of brain NUCB2 mRNA expression to that in the brain of fed rats (Figure 7A). Serum nesfatin-1/NUCB2 levels in rats deprived of food for 24 h were significantly lower compared to that in ad libitum fed rats (Figure 7B). Levels of circulating nesfatin-1/NUCB2 significantly increased upon feeding (at 2200 h; 3 h after commencing the dark phase), compared to that found at a time immediately prior to the dark phase (at 1800 h; final hour of the light phase) (Figure 7C).

Bottom Line: Nesfatin-1, the N-terminal fragment of nucleobindin 2 (NUCB2), is an 82 amino-acid peptide that inhibits food intake and exerts weight-reducing effects.On the seventh day of nesfatin-1 infusion, cumulative food intake, and total spontaneous physical activity during the dark phase were significantly reduced and elevated, respectively.Collectively, our results indicate that chronic peripheral administration of nesfatin-1 at the dose tested, results in a sustained reduction in food intake and modulation of whole body energy homeostasis.

View Article: PubMed Central - PubMed

Affiliation: Department of Veterinary Biomedical Sciences, Western College of Veterinary Medicine, University of Saskatchewan Saskatoon, SK, Canada.

ABSTRACT
Nesfatin-1, the N-terminal fragment of nucleobindin 2 (NUCB2), is an 82 amino-acid peptide that inhibits food intake and exerts weight-reducing effects. Nesfatin-1 has been proposed as a potential anti-obesity peptide. However, studies to date have mainly focused on the acute satiety effects of centrally administered nesfatin-1. The main objective of our studies was to characterize the long-term/chronic effects of peripheral administration of nesfatin-1 on whole-body energy balance and metabolic partitioning in male Fischer 344 rats. Short-term (1 day) subcutaneous infusion of nesfatin-1 (50 μg/kg body weight/day) using osmotic mini-pumps increased spontaneous physical activity and whole-body fat oxidation during the dark phase. This was accompanied by decreased food intake and basal metabolic rate compared to saline infused controls. On the seventh day of nesfatin-1 infusion, cumulative food intake, and total spontaneous physical activity during the dark phase were significantly reduced and elevated, respectively. Meanwhile, intraperitoneal injection of nesfatin-1 only caused a dark phase specific reduction in food intake and an increase in physical activity. NUCB2 mRNA expression in the brain and stomach, as well as serum NUCB2 concentrations were significantly reduced after 24 h fasting, while a post-prandial increase in serum NUCB2 was found in ad libitum fed rats. Collectively, our results indicate that chronic peripheral administration of nesfatin-1 at the dose tested, results in a sustained reduction in food intake and modulation of whole body energy homeostasis.

No MeSH data available.


Related in: MedlinePlus