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Long-term infusion of nesfatin-1 causes a sustained regulation of whole-body energy homeostasis of male Fischer 344 rats.

Mortazavi S, Gonzalez R, Ceddia R, Unniappan S - Front Cell Dev Biol (2015)

Bottom Line: Nesfatin-1, the N-terminal fragment of nucleobindin 2 (NUCB2), is an 82 amino-acid peptide that inhibits food intake and exerts weight-reducing effects.On the seventh day of nesfatin-1 infusion, cumulative food intake, and total spontaneous physical activity during the dark phase were significantly reduced and elevated, respectively.Collectively, our results indicate that chronic peripheral administration of nesfatin-1 at the dose tested, results in a sustained reduction in food intake and modulation of whole body energy homeostasis.

View Article: PubMed Central - PubMed

Affiliation: Department of Veterinary Biomedical Sciences, Western College of Veterinary Medicine, University of Saskatchewan Saskatoon, SK, Canada.

ABSTRACT
Nesfatin-1, the N-terminal fragment of nucleobindin 2 (NUCB2), is an 82 amino-acid peptide that inhibits food intake and exerts weight-reducing effects. Nesfatin-1 has been proposed as a potential anti-obesity peptide. However, studies to date have mainly focused on the acute satiety effects of centrally administered nesfatin-1. The main objective of our studies was to characterize the long-term/chronic effects of peripheral administration of nesfatin-1 on whole-body energy balance and metabolic partitioning in male Fischer 344 rats. Short-term (1 day) subcutaneous infusion of nesfatin-1 (50 μg/kg body weight/day) using osmotic mini-pumps increased spontaneous physical activity and whole-body fat oxidation during the dark phase. This was accompanied by decreased food intake and basal metabolic rate compared to saline infused controls. On the seventh day of nesfatin-1 infusion, cumulative food intake, and total spontaneous physical activity during the dark phase were significantly reduced and elevated, respectively. Meanwhile, intraperitoneal injection of nesfatin-1 only caused a dark phase specific reduction in food intake and an increase in physical activity. NUCB2 mRNA expression in the brain and stomach, as well as serum NUCB2 concentrations were significantly reduced after 24 h fasting, while a post-prandial increase in serum NUCB2 was found in ad libitum fed rats. Collectively, our results indicate that chronic peripheral administration of nesfatin-1 at the dose tested, results in a sustained reduction in food intake and modulation of whole body energy homeostasis.

No MeSH data available.


Related in: MedlinePlus

Cumulative food intake (mg/g BW; A) was significantly reduced, but the average feeding bout size (mg/g BW; B) was not influenced by nesfatin-1 during the dark phase. Increases in locomotor activity were also observed in nesfatin-1 treated animals (beam breaks/12 h; C–F) during the dark phase. The activities were presented as total horizontal (X-TOT), ambulatory (X-AMB, which refers to successive beam breaks in the X axis), and vertical (Z-TOT) movements. Dark cycle occurred during 1900 to 0700 h, while the light cycle was from 0700 to 1900 h next day. All data are presented as means ± SEM with an n = 4 rats/group. *P < 0.05 compared to control, **P < 0.01 compared to control, ***P < 0.001 compared to control.
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Figure 5: Cumulative food intake (mg/g BW; A) was significantly reduced, but the average feeding bout size (mg/g BW; B) was not influenced by nesfatin-1 during the dark phase. Increases in locomotor activity were also observed in nesfatin-1 treated animals (beam breaks/12 h; C–F) during the dark phase. The activities were presented as total horizontal (X-TOT), ambulatory (X-AMB, which refers to successive beam breaks in the X axis), and vertical (Z-TOT) movements. Dark cycle occurred during 1900 to 0700 h, while the light cycle was from 0700 to 1900 h next day. All data are presented as means ± SEM with an n = 4 rats/group. *P < 0.05 compared to control, **P < 0.01 compared to control, ***P < 0.001 compared to control.

Mentions: Intraperitoneal injection of nesfatin-1 caused a dark phase specific reduction in food intake (Figure 5A), and physical activity (Figures 5C–F). Unlike the day 1 and day 7 results, nesfatin-1 was found to have no effects on other metabolic parameters tested (Figures 5B, 6A–F).


Long-term infusion of nesfatin-1 causes a sustained regulation of whole-body energy homeostasis of male Fischer 344 rats.

Mortazavi S, Gonzalez R, Ceddia R, Unniappan S - Front Cell Dev Biol (2015)

Cumulative food intake (mg/g BW; A) was significantly reduced, but the average feeding bout size (mg/g BW; B) was not influenced by nesfatin-1 during the dark phase. Increases in locomotor activity were also observed in nesfatin-1 treated animals (beam breaks/12 h; C–F) during the dark phase. The activities were presented as total horizontal (X-TOT), ambulatory (X-AMB, which refers to successive beam breaks in the X axis), and vertical (Z-TOT) movements. Dark cycle occurred during 1900 to 0700 h, while the light cycle was from 0700 to 1900 h next day. All data are presented as means ± SEM with an n = 4 rats/group. *P < 0.05 compared to control, **P < 0.01 compared to control, ***P < 0.001 compared to control.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4389570&req=5

Figure 5: Cumulative food intake (mg/g BW; A) was significantly reduced, but the average feeding bout size (mg/g BW; B) was not influenced by nesfatin-1 during the dark phase. Increases in locomotor activity were also observed in nesfatin-1 treated animals (beam breaks/12 h; C–F) during the dark phase. The activities were presented as total horizontal (X-TOT), ambulatory (X-AMB, which refers to successive beam breaks in the X axis), and vertical (Z-TOT) movements. Dark cycle occurred during 1900 to 0700 h, while the light cycle was from 0700 to 1900 h next day. All data are presented as means ± SEM with an n = 4 rats/group. *P < 0.05 compared to control, **P < 0.01 compared to control, ***P < 0.001 compared to control.
Mentions: Intraperitoneal injection of nesfatin-1 caused a dark phase specific reduction in food intake (Figure 5A), and physical activity (Figures 5C–F). Unlike the day 1 and day 7 results, nesfatin-1 was found to have no effects on other metabolic parameters tested (Figures 5B, 6A–F).

Bottom Line: Nesfatin-1, the N-terminal fragment of nucleobindin 2 (NUCB2), is an 82 amino-acid peptide that inhibits food intake and exerts weight-reducing effects.On the seventh day of nesfatin-1 infusion, cumulative food intake, and total spontaneous physical activity during the dark phase were significantly reduced and elevated, respectively.Collectively, our results indicate that chronic peripheral administration of nesfatin-1 at the dose tested, results in a sustained reduction in food intake and modulation of whole body energy homeostasis.

View Article: PubMed Central - PubMed

Affiliation: Department of Veterinary Biomedical Sciences, Western College of Veterinary Medicine, University of Saskatchewan Saskatoon, SK, Canada.

ABSTRACT
Nesfatin-1, the N-terminal fragment of nucleobindin 2 (NUCB2), is an 82 amino-acid peptide that inhibits food intake and exerts weight-reducing effects. Nesfatin-1 has been proposed as a potential anti-obesity peptide. However, studies to date have mainly focused on the acute satiety effects of centrally administered nesfatin-1. The main objective of our studies was to characterize the long-term/chronic effects of peripheral administration of nesfatin-1 on whole-body energy balance and metabolic partitioning in male Fischer 344 rats. Short-term (1 day) subcutaneous infusion of nesfatin-1 (50 μg/kg body weight/day) using osmotic mini-pumps increased spontaneous physical activity and whole-body fat oxidation during the dark phase. This was accompanied by decreased food intake and basal metabolic rate compared to saline infused controls. On the seventh day of nesfatin-1 infusion, cumulative food intake, and total spontaneous physical activity during the dark phase were significantly reduced and elevated, respectively. Meanwhile, intraperitoneal injection of nesfatin-1 only caused a dark phase specific reduction in food intake and an increase in physical activity. NUCB2 mRNA expression in the brain and stomach, as well as serum NUCB2 concentrations were significantly reduced after 24 h fasting, while a post-prandial increase in serum NUCB2 was found in ad libitum fed rats. Collectively, our results indicate that chronic peripheral administration of nesfatin-1 at the dose tested, results in a sustained reduction in food intake and modulation of whole body energy homeostasis.

No MeSH data available.


Related in: MedlinePlus