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Thymoquinone protects end organs from abdominal aorta ischemia/reperfusion injury in a rat model.

Aydin MS, Kocarslan A, Kocarslan S, Kucuk A, Eser İ, Sezen H, Buyukfirat E, Hazar A - Rev Bras Cir Cardiovasc (2015 Jan-Mar)

Bottom Line: Total oxidative status and oxidative stress index activity in blood samples were statistically higher in the control group compared to the sham and TQ-treatment groups (P<0.001 for TOS and OSI).Control group injury scores were statistically higher compared to sham and TQ-treatment groups (P<0.001 for all comparisons).Thymoquinone administered intraperitoneally was effective in reducing oxidative stress and histopathologic injury in an acute abdominal aorta ischemia-reperfusion rat model.

View Article: PubMed Central - PubMed

Affiliation: Department of Cardiovascular Surgery, Faculty of Medicine, Harran Univercity, Sanliurfa, Turkey.

ABSTRACT

Introduction: Previous studies have demonstrated that thymoquinone has protective effects against ischemia reperfusion injury to various organs like lungs, kidneys and liver in different experimental models.

Objective: We aimed to determine whether thymoquinone has favorable effects on lung, renal, heart tissues and oxidative stress in abdominal aorta ischemia-reperfusion injury.

Methods: Thirty rats were divided into three groups as sham (n=10), control (n=10) and thymoquinone (TQ) treatment group (n=10). Control and TQ-treatment groups underwent abdominal aorta ischemia for 45 minutes followed by a 120-min period of reperfusion. In the TQ-treatment group, thymoquinone was given 5 minutes. before reperfusion at a dose of 20 mg/kg via an intraperitoneal route. Total antioxidant capacity, total oxidative status (TOS), and oxidative stress index (OSI) in blood serum were measured and lung, kidney, and heart tissue histopathology were evaluated with light microscopy.

Results: Total oxidative status and oxidative stress index activity in blood samples were statistically higher in the control group compared to the sham and TQ-treatment groups (P<0.001 for TOS and OSI). Control group injury scores were statistically higher compared to sham and TQ-treatment groups (P<0.001 for all comparisons).

Conclusion: Thymoquinone administered intraperitoneally was effective in reducing oxidative stress and histopathologic injury in an acute abdominal aorta ischemia-reperfusion rat model.

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A and D show renal and lung tissues samples of the sham group and there were nopathological changes. B) shows lung tissues samples of control group andneutrophil and leukocyte infiltration with alveolar congestion were observed. E)shows renal tissues samples of control group and tubular damage characterized bycast formation; the loss of brush border and interstitial edema were observed. Cand F show lung and renal tissues samples of thymoquinine treated group and therewere less severe lesions than in the control group.
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f04: A and D show renal and lung tissues samples of the sham group and there were nopathological changes. B) shows lung tissues samples of control group andneutrophil and leukocyte infiltration with alveolar congestion were observed. E)shows renal tissues samples of control group and tubular damage characterized bycast formation; the loss of brush border and interstitial edema were observed. Cand F show lung and renal tissues samples of thymoquinine treated group and therewere less severe lesions than in the control group.

Mentions: Upon histopathological evaluation, renal, lung and heart tissues were found to be normalwith no pathological changes in the sham group (Figures4A and 4D). Histopathologicalexamination of the tissues in the control group revealed severe lesions, such as tubulardamage characterized by cast formation, the loss of brush border and interstitial edemain the kidney. Histopathological examination of the tissues in the control grouprevealed neutrophil and leukocyte infiltration with alveolar congestion in the lung.Histopathological examination of the tissues in the control group revealed interstitialedema in the heart (Figures 4B and 4E). In rats receiving TQ intraperitoneally, theselesions were less severe than in the control group (Figures 4C and 4F).


Thymoquinone protects end organs from abdominal aorta ischemia/reperfusion injury in a rat model.

Aydin MS, Kocarslan A, Kocarslan S, Kucuk A, Eser İ, Sezen H, Buyukfirat E, Hazar A - Rev Bras Cir Cardiovasc (2015 Jan-Mar)

A and D show renal and lung tissues samples of the sham group and there were nopathological changes. B) shows lung tissues samples of control group andneutrophil and leukocyte infiltration with alveolar congestion were observed. E)shows renal tissues samples of control group and tubular damage characterized bycast formation; the loss of brush border and interstitial edema were observed. Cand F show lung and renal tissues samples of thymoquinine treated group and therewere less severe lesions than in the control group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4389520&req=5

f04: A and D show renal and lung tissues samples of the sham group and there were nopathological changes. B) shows lung tissues samples of control group andneutrophil and leukocyte infiltration with alveolar congestion were observed. E)shows renal tissues samples of control group and tubular damage characterized bycast formation; the loss of brush border and interstitial edema were observed. Cand F show lung and renal tissues samples of thymoquinine treated group and therewere less severe lesions than in the control group.
Mentions: Upon histopathological evaluation, renal, lung and heart tissues were found to be normalwith no pathological changes in the sham group (Figures4A and 4D). Histopathologicalexamination of the tissues in the control group revealed severe lesions, such as tubulardamage characterized by cast formation, the loss of brush border and interstitial edemain the kidney. Histopathological examination of the tissues in the control grouprevealed neutrophil and leukocyte infiltration with alveolar congestion in the lung.Histopathological examination of the tissues in the control group revealed interstitialedema in the heart (Figures 4B and 4E). In rats receiving TQ intraperitoneally, theselesions were less severe than in the control group (Figures 4C and 4F).

Bottom Line: Total oxidative status and oxidative stress index activity in blood samples were statistically higher in the control group compared to the sham and TQ-treatment groups (P<0.001 for TOS and OSI).Control group injury scores were statistically higher compared to sham and TQ-treatment groups (P<0.001 for all comparisons).Thymoquinone administered intraperitoneally was effective in reducing oxidative stress and histopathologic injury in an acute abdominal aorta ischemia-reperfusion rat model.

View Article: PubMed Central - PubMed

Affiliation: Department of Cardiovascular Surgery, Faculty of Medicine, Harran Univercity, Sanliurfa, Turkey.

ABSTRACT

Introduction: Previous studies have demonstrated that thymoquinone has protective effects against ischemia reperfusion injury to various organs like lungs, kidneys and liver in different experimental models.

Objective: We aimed to determine whether thymoquinone has favorable effects on lung, renal, heart tissues and oxidative stress in abdominal aorta ischemia-reperfusion injury.

Methods: Thirty rats were divided into three groups as sham (n=10), control (n=10) and thymoquinone (TQ) treatment group (n=10). Control and TQ-treatment groups underwent abdominal aorta ischemia for 45 minutes followed by a 120-min period of reperfusion. In the TQ-treatment group, thymoquinone was given 5 minutes. before reperfusion at a dose of 20 mg/kg via an intraperitoneal route. Total antioxidant capacity, total oxidative status (TOS), and oxidative stress index (OSI) in blood serum were measured and lung, kidney, and heart tissue histopathology were evaluated with light microscopy.

Results: Total oxidative status and oxidative stress index activity in blood samples were statistically higher in the control group compared to the sham and TQ-treatment groups (P<0.001 for TOS and OSI). Control group injury scores were statistically higher compared to sham and TQ-treatment groups (P<0.001 for all comparisons).

Conclusion: Thymoquinone administered intraperitoneally was effective in reducing oxidative stress and histopathologic injury in an acute abdominal aorta ischemia-reperfusion rat model.

Show MeSH
Related in: MedlinePlus