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Thymoquinone protects end organs from abdominal aorta ischemia/reperfusion injury in a rat model.

Aydin MS, Kocarslan A, Kocarslan S, Kucuk A, Eser İ, Sezen H, Buyukfirat E, Hazar A - Rev Bras Cir Cardiovasc (2015 Jan-Mar)

Bottom Line: Total oxidative status and oxidative stress index activity in blood samples were statistically higher in the control group compared to the sham and TQ-treatment groups (P<0.001 for TOS and OSI).Control group injury scores were statistically higher compared to sham and TQ-treatment groups (P<0.001 for all comparisons).Thymoquinone administered intraperitoneally was effective in reducing oxidative stress and histopathologic injury in an acute abdominal aorta ischemia-reperfusion rat model.

View Article: PubMed Central - PubMed

Affiliation: Department of Cardiovascular Surgery, Faculty of Medicine, Harran Univercity, Sanliurfa, Turkey.

ABSTRACT

Introduction: Previous studies have demonstrated that thymoquinone has protective effects against ischemia reperfusion injury to various organs like lungs, kidneys and liver in different experimental models.

Objective: We aimed to determine whether thymoquinone has favorable effects on lung, renal, heart tissues and oxidative stress in abdominal aorta ischemia-reperfusion injury.

Methods: Thirty rats were divided into three groups as sham (n=10), control (n=10) and thymoquinone (TQ) treatment group (n=10). Control and TQ-treatment groups underwent abdominal aorta ischemia for 45 minutes followed by a 120-min period of reperfusion. In the TQ-treatment group, thymoquinone was given 5 minutes. before reperfusion at a dose of 20 mg/kg via an intraperitoneal route. Total antioxidant capacity, total oxidative status (TOS), and oxidative stress index (OSI) in blood serum were measured and lung, kidney, and heart tissue histopathology were evaluated with light microscopy.

Results: Total oxidative status and oxidative stress index activity in blood samples were statistically higher in the control group compared to the sham and TQ-treatment groups (P<0.001 for TOS and OSI). Control group injury scores were statistically higher compared to sham and TQ-treatment groups (P<0.001 for all comparisons).

Conclusion: Thymoquinone administered intraperitoneally was effective in reducing oxidative stress and histopathologic injury in an acute abdominal aorta ischemia-reperfusion rat model.

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TOS levels in sham, control, and thymoquinine groups. + P<0.001 (for allcomparisons) compared with sham and thymoquinine groups
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f02: TOS levels in sham, control, and thymoquinine groups. + P<0.001 (for allcomparisons) compared with sham and thymoquinine groups

Mentions: All animals survived through the experimental protocol. TAC activity in blood sampleswere significantly higher in the sham group than in the treatment and control groups(P<0.001; for all comparisons) but there were no statisticallysignificant differences between the treatment group and control group for TAC activity(P>0.05). TOS and OSI activity in blood samples werestatistically higher in the control group than in the sham and thymoquinone group(P<0.001 for all comparisons). Histopathologic injury scores ofrenal, lung and heart tissues are summarized in Table1. Control group injury scores were statistically increased compared to shamand thymoquinone groups (P<0.001 for all comparisons). The resultsare summarized in Figures 1, 2, and 3.


Thymoquinone protects end organs from abdominal aorta ischemia/reperfusion injury in a rat model.

Aydin MS, Kocarslan A, Kocarslan S, Kucuk A, Eser İ, Sezen H, Buyukfirat E, Hazar A - Rev Bras Cir Cardiovasc (2015 Jan-Mar)

TOS levels in sham, control, and thymoquinine groups. + P<0.001 (for allcomparisons) compared with sham and thymoquinine groups
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4389520&req=5

f02: TOS levels in sham, control, and thymoquinine groups. + P<0.001 (for allcomparisons) compared with sham and thymoquinine groups
Mentions: All animals survived through the experimental protocol. TAC activity in blood sampleswere significantly higher in the sham group than in the treatment and control groups(P<0.001; for all comparisons) but there were no statisticallysignificant differences between the treatment group and control group for TAC activity(P>0.05). TOS and OSI activity in blood samples werestatistically higher in the control group than in the sham and thymoquinone group(P<0.001 for all comparisons). Histopathologic injury scores ofrenal, lung and heart tissues are summarized in Table1. Control group injury scores were statistically increased compared to shamand thymoquinone groups (P<0.001 for all comparisons). The resultsare summarized in Figures 1, 2, and 3.

Bottom Line: Total oxidative status and oxidative stress index activity in blood samples were statistically higher in the control group compared to the sham and TQ-treatment groups (P<0.001 for TOS and OSI).Control group injury scores were statistically higher compared to sham and TQ-treatment groups (P<0.001 for all comparisons).Thymoquinone administered intraperitoneally was effective in reducing oxidative stress and histopathologic injury in an acute abdominal aorta ischemia-reperfusion rat model.

View Article: PubMed Central - PubMed

Affiliation: Department of Cardiovascular Surgery, Faculty of Medicine, Harran Univercity, Sanliurfa, Turkey.

ABSTRACT

Introduction: Previous studies have demonstrated that thymoquinone has protective effects against ischemia reperfusion injury to various organs like lungs, kidneys and liver in different experimental models.

Objective: We aimed to determine whether thymoquinone has favorable effects on lung, renal, heart tissues and oxidative stress in abdominal aorta ischemia-reperfusion injury.

Methods: Thirty rats were divided into three groups as sham (n=10), control (n=10) and thymoquinone (TQ) treatment group (n=10). Control and TQ-treatment groups underwent abdominal aorta ischemia for 45 minutes followed by a 120-min period of reperfusion. In the TQ-treatment group, thymoquinone was given 5 minutes. before reperfusion at a dose of 20 mg/kg via an intraperitoneal route. Total antioxidant capacity, total oxidative status (TOS), and oxidative stress index (OSI) in blood serum were measured and lung, kidney, and heart tissue histopathology were evaluated with light microscopy.

Results: Total oxidative status and oxidative stress index activity in blood samples were statistically higher in the control group compared to the sham and TQ-treatment groups (P<0.001 for TOS and OSI). Control group injury scores were statistically higher compared to sham and TQ-treatment groups (P<0.001 for all comparisons).

Conclusion: Thymoquinone administered intraperitoneally was effective in reducing oxidative stress and histopathologic injury in an acute abdominal aorta ischemia-reperfusion rat model.

Show MeSH
Related in: MedlinePlus