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Development of cardioplegic solution without potassium: experimental study in rat.

Reichert K, Carmo HR, Lima F, Torina AG, Vilarinho KA, Oliveira PP, Silveira Filho LM, Severino ES, Petrucci O - Rev Bras Cir Cardiovasc (2013 Oct-Dec)

Bottom Line: The caspase activity was lower in the HTK group compared to LIRM and G/A solutions (P<0.01).The LIRM solution showed lower caspase activity compared to Braile solution (P<0.01).More evaluation is necessary for an optimal cardioplegic solution development.

View Article: PubMed Central - PubMed

ABSTRACT

Introduction: Myocardial preservation during open heart surgeries and harvesting for transplant are of great importance. The heart at the end of procedure has to resume its functions as soon as possible. All cardioplegic solutions are based on potassium for induction of cardioplegic arrest.

Objective: To assess a cardioplegic solution with no potassium addition to the formula with two other commercially available cardioplegic solutions. The comparative assessment was based on cytotoxicity, adenosine triphosphate myocardial preservation, and caspase 3 activity. The tested solution (LIRM) uses low doses of sodium channel blocker (lidocaine), potassium channel opener (cromakalin), and actin/myosin cross bridge inhibitor (2,3-butanedione monoxime).

Methods: Wistar rats underwent thoracotomy under mechanical ventilation and three different solutions were used for "in situ" perfusion for cardioplegic arrest induction: Custodiol (HTK), Braile (G/A), and LIRM solutions. After cardiac arrest, the hearts were excised and kept in cold storage for 4 hours. After this period, the hearts were assessed with optical light microscopy, myocardial ATP content and caspase 3 activity. All three solutions were evaluated for direct cytotoxicity with L929 and WEHI-164 cells.

Results: The ATP content was higher in the Custodiol group compared to two other solutions (P<0.05). The caspase activity was lower in the HTK group compared to LIRM and G/A solutions (P<0.01). The LIRM solution showed lower caspase activity compared to Braile solution (P<0.01). All solutions showed no cytotoxicity effect after 24 hours of cells exposure to cardioplegic solutions.

Conclusion: Cardioplegia solutions without potassium are promised and aminoacid addition might be an interesting strategy. More evaluation is necessary for an optimal cardioplegic solution development.

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Related in: MedlinePlus

The ATP myocardial content was higher in the HTK group compared to the othergroups (A). Comparison of caspase 3 activity after 4 hours of cold storage (B).All values are expressed as the means; bars indicate standard error of themean. Differences were assessed by one-way ANOVA. n=5 animals per group
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f02: The ATP myocardial content was higher in the HTK group compared to the othergroups (A). Comparison of caspase 3 activity after 4 hours of cold storage (B).All values are expressed as the means; bars indicate standard error of themean. Differences were assessed by one-way ANOVA. n=5 animals per group

Mentions: The ATP myocardial content after 4 hours of cold storage was higher in the HTKsolution group, with no differences between the LIRM and G/A groups (Figure 2A). The caspase 3 activity was higher inthe G/A group compared to that of the LIRM and HTK groups, whereas the LIRM groupshowed higher caspase activity compared to the HTK group (Figure 2B).


Development of cardioplegic solution without potassium: experimental study in rat.

Reichert K, Carmo HR, Lima F, Torina AG, Vilarinho KA, Oliveira PP, Silveira Filho LM, Severino ES, Petrucci O - Rev Bras Cir Cardiovasc (2013 Oct-Dec)

The ATP myocardial content was higher in the HTK group compared to the othergroups (A). Comparison of caspase 3 activity after 4 hours of cold storage (B).All values are expressed as the means; bars indicate standard error of themean. Differences were assessed by one-way ANOVA. n=5 animals per group
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4389432&req=5

f02: The ATP myocardial content was higher in the HTK group compared to the othergroups (A). Comparison of caspase 3 activity after 4 hours of cold storage (B).All values are expressed as the means; bars indicate standard error of themean. Differences were assessed by one-way ANOVA. n=5 animals per group
Mentions: The ATP myocardial content after 4 hours of cold storage was higher in the HTKsolution group, with no differences between the LIRM and G/A groups (Figure 2A). The caspase 3 activity was higher inthe G/A group compared to that of the LIRM and HTK groups, whereas the LIRM groupshowed higher caspase activity compared to the HTK group (Figure 2B).

Bottom Line: The caspase activity was lower in the HTK group compared to LIRM and G/A solutions (P<0.01).The LIRM solution showed lower caspase activity compared to Braile solution (P<0.01).More evaluation is necessary for an optimal cardioplegic solution development.

View Article: PubMed Central - PubMed

ABSTRACT

Introduction: Myocardial preservation during open heart surgeries and harvesting for transplant are of great importance. The heart at the end of procedure has to resume its functions as soon as possible. All cardioplegic solutions are based on potassium for induction of cardioplegic arrest.

Objective: To assess a cardioplegic solution with no potassium addition to the formula with two other commercially available cardioplegic solutions. The comparative assessment was based on cytotoxicity, adenosine triphosphate myocardial preservation, and caspase 3 activity. The tested solution (LIRM) uses low doses of sodium channel blocker (lidocaine), potassium channel opener (cromakalin), and actin/myosin cross bridge inhibitor (2,3-butanedione monoxime).

Methods: Wistar rats underwent thoracotomy under mechanical ventilation and three different solutions were used for "in situ" perfusion for cardioplegic arrest induction: Custodiol (HTK), Braile (G/A), and LIRM solutions. After cardiac arrest, the hearts were excised and kept in cold storage for 4 hours. After this period, the hearts were assessed with optical light microscopy, myocardial ATP content and caspase 3 activity. All three solutions were evaluated for direct cytotoxicity with L929 and WEHI-164 cells.

Results: The ATP content was higher in the Custodiol group compared to two other solutions (P<0.05). The caspase activity was lower in the HTK group compared to LIRM and G/A solutions (P<0.01). The LIRM solution showed lower caspase activity compared to Braile solution (P<0.01). All solutions showed no cytotoxicity effect after 24 hours of cells exposure to cardioplegic solutions.

Conclusion: Cardioplegia solutions without potassium are promised and aminoacid addition might be an interesting strategy. More evaluation is necessary for an optimal cardioplegic solution development.

Show MeSH
Related in: MedlinePlus