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ADSCs on PLLA/PCL Hybrid Nanoscaffold and Gelatin Modification: Cytocompatibility and Mechanical Properties.

Mashhadikhan M, Soleimani M, Parivar K, Yaghmaei P - Avicenna J Med Biotechnol (2015 Jan-Mar)

Bottom Line: Development of tissue engineering and regenerative medicine has led to designing scaffolds and their modification to provide a better microenvironment which mimics the natural niche of the cells.Gelatin surface modification was applied to improve scaffold flexibility and cytocompatibility.ADSCs were seeded after O2 plasma treatment and gelatin coating of the scaffolds.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Faculty of Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran.

ABSTRACT

Background: Development of tissue engineering and regenerative medicine has led to designing scaffolds and their modification to provide a better microenvironment which mimics the natural niche of the cells. Gelatin surface modification was applied to improve scaffold flexibility and cytocompatibility.

Methods: PLLA/PCL aligned fibrous scaffold was fabricated using electrospinning method. ADSCs were seeded after O2 plasma treatment and gelatin coating of the scaffolds. The morphological and mechanical properties of blends were assessed by Scanning Electron Microscopy (SEM), tensile test and ATR-FTIR. The cells proliferation was evaluated by MTT assay.

Results: Based on the results, it is supposed that gelatin coating is a brilliant method of surface modification which significantly increases the mechanical properties of scaffold without any changes on the construction or on the direction of nanofibers which conducts cell's elongation. MTT analysis exhibited that ADSCs attachment, viability and proliferation significantly (p < 0.05) increased after gelatin treatment.

Conclusion: Gelatin surface modification is a highly beneficial method to improve cytocompatibility, flexibility and mechanical features of the scaffolds which doesn't affect the nanofibers construction. Proliferation of Adipose Derived Stem Cells (ADSCs) as a remarkable source of stem cells was investigated for the first time on PLLA/PCL hybrid scaffold.

No MeSH data available.


Related in: MedlinePlus

MTT assay of ADSCs proliferation and viability on PLLA/PCL scaffolds with or without gelatin coating and TCPS during 5 days of culture. Significant increase in cell's OD levels is indicated with * at <0.05.
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Figure 0005: MTT assay of ADSCs proliferation and viability on PLLA/PCL scaffolds with or without gelatin coating and TCPS during 5 days of culture. Significant increase in cell's OD levels is indicated with * at <0.05.

Mentions: ADSCs showed significantly (p < 0.05) higher proliferation rate and viability when they were seeded on PLLA/PCL hybrid in comparison to 24-well Tissue Culture Polystyrene (TCPS) after day fourth, even when the scaffolds were not coated with gelatin. The results of MTT assay suggest that structure of nano-fibrous scaffolds improved the cell's viability. Moreover, MTT results indicated that cell proliferation and viability were augmented when gelatin coating was applied, as it demonstrates significant (p < 0.05) increase from day 3 in comparison with both others. It means that gelatin provides a suitable substrate for cell attachment and proliferation compared to uncoated scaffolds (Figure 5).


ADSCs on PLLA/PCL Hybrid Nanoscaffold and Gelatin Modification: Cytocompatibility and Mechanical Properties.

Mashhadikhan M, Soleimani M, Parivar K, Yaghmaei P - Avicenna J Med Biotechnol (2015 Jan-Mar)

MTT assay of ADSCs proliferation and viability on PLLA/PCL scaffolds with or without gelatin coating and TCPS during 5 days of culture. Significant increase in cell's OD levels is indicated with * at <0.05.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4388888&req=5

Figure 0005: MTT assay of ADSCs proliferation and viability on PLLA/PCL scaffolds with or without gelatin coating and TCPS during 5 days of culture. Significant increase in cell's OD levels is indicated with * at <0.05.
Mentions: ADSCs showed significantly (p < 0.05) higher proliferation rate and viability when they were seeded on PLLA/PCL hybrid in comparison to 24-well Tissue Culture Polystyrene (TCPS) after day fourth, even when the scaffolds were not coated with gelatin. The results of MTT assay suggest that structure of nano-fibrous scaffolds improved the cell's viability. Moreover, MTT results indicated that cell proliferation and viability were augmented when gelatin coating was applied, as it demonstrates significant (p < 0.05) increase from day 3 in comparison with both others. It means that gelatin provides a suitable substrate for cell attachment and proliferation compared to uncoated scaffolds (Figure 5).

Bottom Line: Development of tissue engineering and regenerative medicine has led to designing scaffolds and their modification to provide a better microenvironment which mimics the natural niche of the cells.Gelatin surface modification was applied to improve scaffold flexibility and cytocompatibility.ADSCs were seeded after O2 plasma treatment and gelatin coating of the scaffolds.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Faculty of Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran.

ABSTRACT

Background: Development of tissue engineering and regenerative medicine has led to designing scaffolds and their modification to provide a better microenvironment which mimics the natural niche of the cells. Gelatin surface modification was applied to improve scaffold flexibility and cytocompatibility.

Methods: PLLA/PCL aligned fibrous scaffold was fabricated using electrospinning method. ADSCs were seeded after O2 plasma treatment and gelatin coating of the scaffolds. The morphological and mechanical properties of blends were assessed by Scanning Electron Microscopy (SEM), tensile test and ATR-FTIR. The cells proliferation was evaluated by MTT assay.

Results: Based on the results, it is supposed that gelatin coating is a brilliant method of surface modification which significantly increases the mechanical properties of scaffold without any changes on the construction or on the direction of nanofibers which conducts cell's elongation. MTT analysis exhibited that ADSCs attachment, viability and proliferation significantly (p < 0.05) increased after gelatin treatment.

Conclusion: Gelatin surface modification is a highly beneficial method to improve cytocompatibility, flexibility and mechanical features of the scaffolds which doesn't affect the nanofibers construction. Proliferation of Adipose Derived Stem Cells (ADSCs) as a remarkable source of stem cells was investigated for the first time on PLLA/PCL hybrid scaffold.

No MeSH data available.


Related in: MedlinePlus