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ADSCs on PLLA/PCL Hybrid Nanoscaffold and Gelatin Modification: Cytocompatibility and Mechanical Properties.

Mashhadikhan M, Soleimani M, Parivar K, Yaghmaei P - Avicenna J Med Biotechnol (2015 Jan-Mar)

Bottom Line: Based on the results, it is supposed that gelatin coating is a brilliant method of surface modification which significantly increases the mechanical properties of scaffold without any changes on the construction or on the direction of nanofibers which conducts cell's elongation.MTT analysis exhibited that ADSCs attachment, viability and proliferation significantly (p < 0.05) increased after gelatin treatment.Gelatin surface modification is a highly beneficial method to improve cytocompatibility, flexibility and mechanical features of the scaffolds which doesn't affect the nanofibers construction.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Faculty of Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran.

ABSTRACT

Background: Development of tissue engineering and regenerative medicine has led to designing scaffolds and their modification to provide a better microenvironment which mimics the natural niche of the cells. Gelatin surface modification was applied to improve scaffold flexibility and cytocompatibility.

Methods: PLLA/PCL aligned fibrous scaffold was fabricated using electrospinning method. ADSCs were seeded after O2 plasma treatment and gelatin coating of the scaffolds. The morphological and mechanical properties of blends were assessed by Scanning Electron Microscopy (SEM), tensile test and ATR-FTIR. The cells proliferation was evaluated by MTT assay.

Results: Based on the results, it is supposed that gelatin coating is a brilliant method of surface modification which significantly increases the mechanical properties of scaffold without any changes on the construction or on the direction of nanofibers which conducts cell's elongation. MTT analysis exhibited that ADSCs attachment, viability and proliferation significantly (p < 0.05) increased after gelatin treatment.

Conclusion: Gelatin surface modification is a highly beneficial method to improve cytocompatibility, flexibility and mechanical features of the scaffolds which doesn't affect the nanofibers construction. Proliferation of Adipose Derived Stem Cells (ADSCs) as a remarkable source of stem cells was investigated for the first time on PLLA/PCL hybrid scaffold.

No MeSH data available.


Stress-strain of PLLA/PCL compared with gelatin coated samples. The samples numbered 1-3 are related to uncoated and 4-6 to gelatin-coated hybrids.
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Figure 0004: Stress-strain of PLLA/PCL compared with gelatin coated samples. The samples numbered 1-3 are related to uncoated and 4-6 to gelatin-coated hybrids.

Mentions: Figure 4 illustrates tensile stress-strain curves of PLLA/PCL blends. The non-linear stress-elongation at break curves exhibit an increase in peak of stress when the samples are coated with gelatin in comparison with uncoated ones and this suggests that gelatin increases mechanical properties of the blends. However, data analysis indicates this increase is not remarkable for peak stress (p = 0.26) and/or break strain (p = 0.63). Indeed, gelatin modulates tensile properties without damage to mechanical strength of the hybrid.


ADSCs on PLLA/PCL Hybrid Nanoscaffold and Gelatin Modification: Cytocompatibility and Mechanical Properties.

Mashhadikhan M, Soleimani M, Parivar K, Yaghmaei P - Avicenna J Med Biotechnol (2015 Jan-Mar)

Stress-strain of PLLA/PCL compared with gelatin coated samples. The samples numbered 1-3 are related to uncoated and 4-6 to gelatin-coated hybrids.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4388888&req=5

Figure 0004: Stress-strain of PLLA/PCL compared with gelatin coated samples. The samples numbered 1-3 are related to uncoated and 4-6 to gelatin-coated hybrids.
Mentions: Figure 4 illustrates tensile stress-strain curves of PLLA/PCL blends. The non-linear stress-elongation at break curves exhibit an increase in peak of stress when the samples are coated with gelatin in comparison with uncoated ones and this suggests that gelatin increases mechanical properties of the blends. However, data analysis indicates this increase is not remarkable for peak stress (p = 0.26) and/or break strain (p = 0.63). Indeed, gelatin modulates tensile properties without damage to mechanical strength of the hybrid.

Bottom Line: Based on the results, it is supposed that gelatin coating is a brilliant method of surface modification which significantly increases the mechanical properties of scaffold without any changes on the construction or on the direction of nanofibers which conducts cell's elongation.MTT analysis exhibited that ADSCs attachment, viability and proliferation significantly (p < 0.05) increased after gelatin treatment.Gelatin surface modification is a highly beneficial method to improve cytocompatibility, flexibility and mechanical features of the scaffolds which doesn't affect the nanofibers construction.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Faculty of Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran.

ABSTRACT

Background: Development of tissue engineering and regenerative medicine has led to designing scaffolds and their modification to provide a better microenvironment which mimics the natural niche of the cells. Gelatin surface modification was applied to improve scaffold flexibility and cytocompatibility.

Methods: PLLA/PCL aligned fibrous scaffold was fabricated using electrospinning method. ADSCs were seeded after O2 plasma treatment and gelatin coating of the scaffolds. The morphological and mechanical properties of blends were assessed by Scanning Electron Microscopy (SEM), tensile test and ATR-FTIR. The cells proliferation was evaluated by MTT assay.

Results: Based on the results, it is supposed that gelatin coating is a brilliant method of surface modification which significantly increases the mechanical properties of scaffold without any changes on the construction or on the direction of nanofibers which conducts cell's elongation. MTT analysis exhibited that ADSCs attachment, viability and proliferation significantly (p < 0.05) increased after gelatin treatment.

Conclusion: Gelatin surface modification is a highly beneficial method to improve cytocompatibility, flexibility and mechanical features of the scaffolds which doesn't affect the nanofibers construction. Proliferation of Adipose Derived Stem Cells (ADSCs) as a remarkable source of stem cells was investigated for the first time on PLLA/PCL hybrid scaffold.

No MeSH data available.