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ADSCs on PLLA/PCL Hybrid Nanoscaffold and Gelatin Modification: Cytocompatibility and Mechanical Properties.

Mashhadikhan M, Soleimani M, Parivar K, Yaghmaei P - Avicenna J Med Biotechnol (2015 Jan-Mar)

Bottom Line: Development of tissue engineering and regenerative medicine has led to designing scaffolds and their modification to provide a better microenvironment which mimics the natural niche of the cells.Gelatin surface modification was applied to improve scaffold flexibility and cytocompatibility.ADSCs were seeded after O2 plasma treatment and gelatin coating of the scaffolds.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Faculty of Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran.

ABSTRACT

Background: Development of tissue engineering and regenerative medicine has led to designing scaffolds and their modification to provide a better microenvironment which mimics the natural niche of the cells. Gelatin surface modification was applied to improve scaffold flexibility and cytocompatibility.

Methods: PLLA/PCL aligned fibrous scaffold was fabricated using electrospinning method. ADSCs were seeded after O2 plasma treatment and gelatin coating of the scaffolds. The morphological and mechanical properties of blends were assessed by Scanning Electron Microscopy (SEM), tensile test and ATR-FTIR. The cells proliferation was evaluated by MTT assay.

Results: Based on the results, it is supposed that gelatin coating is a brilliant method of surface modification which significantly increases the mechanical properties of scaffold without any changes on the construction or on the direction of nanofibers which conducts cell's elongation. MTT analysis exhibited that ADSCs attachment, viability and proliferation significantly (p < 0.05) increased after gelatin treatment.

Conclusion: Gelatin surface modification is a highly beneficial method to improve cytocompatibility, flexibility and mechanical features of the scaffolds which doesn't affect the nanofibers construction. Proliferation of Adipose Derived Stem Cells (ADSCs) as a remarkable source of stem cells was investigated for the first time on PLLA/PCL hybrid scaffold.

No MeSH data available.


Related in: MedlinePlus

ATR-FTIR spectra of PLLA/PCL and gelatin coated PLLA/PCL. The * indicates characteristic peaks of PLLA/PCL and # indicates peak for Amide III of gelatin.
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Figure 0003: ATR-FTIR spectra of PLLA/PCL and gelatin coated PLLA/PCL. The * indicates characteristic peaks of PLLA/PCL and # indicates peak for Amide III of gelatin.

Mentions: ATR- FTIR was performed to confirm the existence of gelatin on the surface of PLLA/PCL blends (Figure 3). The peak at 2946 cm-1 corresponded to C-H stretch while at 1735 cm-1 it was for C = O bond and the C-O bending peak appeared at 1182 cm-1. However, the most characteristic peaks of PLLA/PCL were in vicinity of Amide I (1640 cm-1), Amide II (1540 cm-1) and even Amide B (2930 cm-1) of gelatin and the gelatin concentration was so diluted, but the results displayed that a new peak appeared at 1237 cm-1 which confirmed the features of gelatin for Amide III (C-N stretch plus N-H in phase bonding). The asterisks indicate the characteristic peaks of PLLA/PCL in both spectra as if no significant change occurs by gelatin coating.


ADSCs on PLLA/PCL Hybrid Nanoscaffold and Gelatin Modification: Cytocompatibility and Mechanical Properties.

Mashhadikhan M, Soleimani M, Parivar K, Yaghmaei P - Avicenna J Med Biotechnol (2015 Jan-Mar)

ATR-FTIR spectra of PLLA/PCL and gelatin coated PLLA/PCL. The * indicates characteristic peaks of PLLA/PCL and # indicates peak for Amide III of gelatin.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4388888&req=5

Figure 0003: ATR-FTIR spectra of PLLA/PCL and gelatin coated PLLA/PCL. The * indicates characteristic peaks of PLLA/PCL and # indicates peak for Amide III of gelatin.
Mentions: ATR- FTIR was performed to confirm the existence of gelatin on the surface of PLLA/PCL blends (Figure 3). The peak at 2946 cm-1 corresponded to C-H stretch while at 1735 cm-1 it was for C = O bond and the C-O bending peak appeared at 1182 cm-1. However, the most characteristic peaks of PLLA/PCL were in vicinity of Amide I (1640 cm-1), Amide II (1540 cm-1) and even Amide B (2930 cm-1) of gelatin and the gelatin concentration was so diluted, but the results displayed that a new peak appeared at 1237 cm-1 which confirmed the features of gelatin for Amide III (C-N stretch plus N-H in phase bonding). The asterisks indicate the characteristic peaks of PLLA/PCL in both spectra as if no significant change occurs by gelatin coating.

Bottom Line: Development of tissue engineering and regenerative medicine has led to designing scaffolds and their modification to provide a better microenvironment which mimics the natural niche of the cells.Gelatin surface modification was applied to improve scaffold flexibility and cytocompatibility.ADSCs were seeded after O2 plasma treatment and gelatin coating of the scaffolds.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Faculty of Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran.

ABSTRACT

Background: Development of tissue engineering and regenerative medicine has led to designing scaffolds and their modification to provide a better microenvironment which mimics the natural niche of the cells. Gelatin surface modification was applied to improve scaffold flexibility and cytocompatibility.

Methods: PLLA/PCL aligned fibrous scaffold was fabricated using electrospinning method. ADSCs were seeded after O2 plasma treatment and gelatin coating of the scaffolds. The morphological and mechanical properties of blends were assessed by Scanning Electron Microscopy (SEM), tensile test and ATR-FTIR. The cells proliferation was evaluated by MTT assay.

Results: Based on the results, it is supposed that gelatin coating is a brilliant method of surface modification which significantly increases the mechanical properties of scaffold without any changes on the construction or on the direction of nanofibers which conducts cell's elongation. MTT analysis exhibited that ADSCs attachment, viability and proliferation significantly (p < 0.05) increased after gelatin treatment.

Conclusion: Gelatin surface modification is a highly beneficial method to improve cytocompatibility, flexibility and mechanical features of the scaffolds which doesn't affect the nanofibers construction. Proliferation of Adipose Derived Stem Cells (ADSCs) as a remarkable source of stem cells was investigated for the first time on PLLA/PCL hybrid scaffold.

No MeSH data available.


Related in: MedlinePlus