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ADSCs on PLLA/PCL Hybrid Nanoscaffold and Gelatin Modification: Cytocompatibility and Mechanical Properties.

Mashhadikhan M, Soleimani M, Parivar K, Yaghmaei P - Avicenna J Med Biotechnol (2015 Jan-Mar)

Bottom Line: Based on the results, it is supposed that gelatin coating is a brilliant method of surface modification which significantly increases the mechanical properties of scaffold without any changes on the construction or on the direction of nanofibers which conducts cell's elongation.MTT analysis exhibited that ADSCs attachment, viability and proliferation significantly (p < 0.05) increased after gelatin treatment.Gelatin surface modification is a highly beneficial method to improve cytocompatibility, flexibility and mechanical features of the scaffolds which doesn't affect the nanofibers construction.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Faculty of Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran.

ABSTRACT

Background: Development of tissue engineering and regenerative medicine has led to designing scaffolds and their modification to provide a better microenvironment which mimics the natural niche of the cells. Gelatin surface modification was applied to improve scaffold flexibility and cytocompatibility.

Methods: PLLA/PCL aligned fibrous scaffold was fabricated using electrospinning method. ADSCs were seeded after O2 plasma treatment and gelatin coating of the scaffolds. The morphological and mechanical properties of blends were assessed by Scanning Electron Microscopy (SEM), tensile test and ATR-FTIR. The cells proliferation was evaluated by MTT assay.

Results: Based on the results, it is supposed that gelatin coating is a brilliant method of surface modification which significantly increases the mechanical properties of scaffold without any changes on the construction or on the direction of nanofibers which conducts cell's elongation. MTT analysis exhibited that ADSCs attachment, viability and proliferation significantly (p < 0.05) increased after gelatin treatment.

Conclusion: Gelatin surface modification is a highly beneficial method to improve cytocompatibility, flexibility and mechanical features of the scaffolds which doesn't affect the nanofibers construction. Proliferation of Adipose Derived Stem Cells (ADSCs) as a remarkable source of stem cells was investigated for the first time on PLLA/PCL hybrid scaffold.

No MeSH data available.


DAPI staining of ADSCs on gelatin coated PLLA/PCL hybrid scaffold (10×).
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Figure 0002: DAPI staining of ADSCs on gelatin coated PLLA/PCL hybrid scaffold (10×).

Mentions: The SEM images illustrate the electrospun PLLA/ PCL hybrid, without (Figure 1A) and with (Figure 1B) gelatin coating. The observation of highly porous fiber structure of scaffolds shows that when gelatin was used to cover the blends, obviously the brut and fragile fibers of blend became more supple and flexible, which shows the elastic construction of ECM. It is perceived that gelatin uniformly softened the roughness and dryness of fibers when it was used to coat the scaffolds. However, gelatin solution perfectly covered the nanofibers though it did not blemish or fill the scaffold's pores distinctly. SEM images also demonstrated that when ADSCs are seeded on scaffolds with parallel pattern, the fibers can conduct the cell's elongation through themselves. The point is that it happens even after just 1 day of cell seeding (Figures 1C and D) and it indicates the powerful effect of nanofiber orientation as an ECM-like structure on cell growth guidance. Comparing ADSCs on gelatin coated (Figure 1F) and gelatin uncoated (Figure 1E) scaffolds, it can be inferred that in both situations, cells get parallel alignment along the axes of the fibers, thus gelatin coating does not impress cell guidance of the nanofibers. To confirm the presence of the cells on gelatin coated PLLA/PCL scaffold, the cells were stained by DAPI (Figure 2).


ADSCs on PLLA/PCL Hybrid Nanoscaffold and Gelatin Modification: Cytocompatibility and Mechanical Properties.

Mashhadikhan M, Soleimani M, Parivar K, Yaghmaei P - Avicenna J Med Biotechnol (2015 Jan-Mar)

DAPI staining of ADSCs on gelatin coated PLLA/PCL hybrid scaffold (10×).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4388888&req=5

Figure 0002: DAPI staining of ADSCs on gelatin coated PLLA/PCL hybrid scaffold (10×).
Mentions: The SEM images illustrate the electrospun PLLA/ PCL hybrid, without (Figure 1A) and with (Figure 1B) gelatin coating. The observation of highly porous fiber structure of scaffolds shows that when gelatin was used to cover the blends, obviously the brut and fragile fibers of blend became more supple and flexible, which shows the elastic construction of ECM. It is perceived that gelatin uniformly softened the roughness and dryness of fibers when it was used to coat the scaffolds. However, gelatin solution perfectly covered the nanofibers though it did not blemish or fill the scaffold's pores distinctly. SEM images also demonstrated that when ADSCs are seeded on scaffolds with parallel pattern, the fibers can conduct the cell's elongation through themselves. The point is that it happens even after just 1 day of cell seeding (Figures 1C and D) and it indicates the powerful effect of nanofiber orientation as an ECM-like structure on cell growth guidance. Comparing ADSCs on gelatin coated (Figure 1F) and gelatin uncoated (Figure 1E) scaffolds, it can be inferred that in both situations, cells get parallel alignment along the axes of the fibers, thus gelatin coating does not impress cell guidance of the nanofibers. To confirm the presence of the cells on gelatin coated PLLA/PCL scaffold, the cells were stained by DAPI (Figure 2).

Bottom Line: Based on the results, it is supposed that gelatin coating is a brilliant method of surface modification which significantly increases the mechanical properties of scaffold without any changes on the construction or on the direction of nanofibers which conducts cell's elongation.MTT analysis exhibited that ADSCs attachment, viability and proliferation significantly (p < 0.05) increased after gelatin treatment.Gelatin surface modification is a highly beneficial method to improve cytocompatibility, flexibility and mechanical features of the scaffolds which doesn't affect the nanofibers construction.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology, Faculty of Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran.

ABSTRACT

Background: Development of tissue engineering and regenerative medicine has led to designing scaffolds and their modification to provide a better microenvironment which mimics the natural niche of the cells. Gelatin surface modification was applied to improve scaffold flexibility and cytocompatibility.

Methods: PLLA/PCL aligned fibrous scaffold was fabricated using electrospinning method. ADSCs were seeded after O2 plasma treatment and gelatin coating of the scaffolds. The morphological and mechanical properties of blends were assessed by Scanning Electron Microscopy (SEM), tensile test and ATR-FTIR. The cells proliferation was evaluated by MTT assay.

Results: Based on the results, it is supposed that gelatin coating is a brilliant method of surface modification which significantly increases the mechanical properties of scaffold without any changes on the construction or on the direction of nanofibers which conducts cell's elongation. MTT analysis exhibited that ADSCs attachment, viability and proliferation significantly (p < 0.05) increased after gelatin treatment.

Conclusion: Gelatin surface modification is a highly beneficial method to improve cytocompatibility, flexibility and mechanical features of the scaffolds which doesn't affect the nanofibers construction. Proliferation of Adipose Derived Stem Cells (ADSCs) as a remarkable source of stem cells was investigated for the first time on PLLA/PCL hybrid scaffold.

No MeSH data available.