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Intravenous transplantation of very small embryonic like stem cells in treatment of diabetes mellitus.

Abouzaripour M, Ragerdi Kashani I, Pasbakhsh P, Atlasy N - Avicenna J Med Biotechnol (2015 Jan-Mar)

Bottom Line: It was proved that CD45-, CXCR4+, and Sca1+ sorted cells express oct4 and SSEA1.Our results revealed that intravenously implanted VSELs could migrate into the pancreas of hosts and survive in the diabetic pancreas.In treated groups, blood glucose decreased significantly for at least two month and the weights of mice increased gradually.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran.

ABSTRACT

Background: Diabetes Mellitus (DM), simply known as diabetes, refers to a group of metabolic diseases in which there are high blood sugar levels over a prolonged period. In this study, the feasibility and safety of intravenous transplantation of Very Small Embryonic Like stem cells (VSELs) were investigated for diabetes repair, and finally the migration and distribution of these cells in hosts were observed.

Methods: Mouse bone marrow VSELs were isolated by Fluorescent Activating Cell Sorting (FACS) method by using fluorescent antibodies against CD45, CXCR4 and Sca1 markers. Sorted cells were analyzed for expression of oct4 and SSEA1 markers with immunocytochemistry staining method. To determine multilineage differentiation, sorted cells were differentiated to Schwann, osteocyte and beta cells. Ten days after the establishment of a mouse model of pancreas necrosis, DiI-labeled VSELs were injected into these mice via tail vein. Pancreases were harvested 4 weeks after transplantation and the sections of these tissues were observed under fluorescent microscope.

Results: It was proved that CD45-, CXCR4+, and Sca1+ sorted cells express oct4 and SSEA1. Our results revealed that intravenously implanted VSELs could migrate into the pancreas of hosts and survive in the diabetic pancreas. In treated groups, blood glucose decreased significantly for at least two month and the weights of mice increased gradually.

Conclusion: This study provides a strategy for using VSELs for curing diabetes and other regenerative diseases, and the strategy is considered an alternative for other stem cell types.

No MeSH data available.


Related in: MedlinePlus

A) Average non-fasting glucose levels of control and experimental diabetic mice. B) Average non-fasting glucose levels of diabetic group, before and after treatment (the mean blood sugar of treated diabetic mice is calculated 3 weeks after cell therapy with VSELs). Asterisks in figure denote statistical significance, (p= 0.0002).
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Figure 0008: A) Average non-fasting glucose levels of control and experimental diabetic mice. B) Average non-fasting glucose levels of diabetic group, before and after treatment (the mean blood sugar of treated diabetic mice is calculated 3 weeks after cell therapy with VSELs). Asterisks in figure denote statistical significance, (p= 0.0002).

Mentions: Within a week after VSEL administration, blood glucose levels significantly decreased until reaching almost euglycemic values a month later. Hyperglycemia correction lasted at least for 2 months (Figure 8).


Intravenous transplantation of very small embryonic like stem cells in treatment of diabetes mellitus.

Abouzaripour M, Ragerdi Kashani I, Pasbakhsh P, Atlasy N - Avicenna J Med Biotechnol (2015 Jan-Mar)

A) Average non-fasting glucose levels of control and experimental diabetic mice. B) Average non-fasting glucose levels of diabetic group, before and after treatment (the mean blood sugar of treated diabetic mice is calculated 3 weeks after cell therapy with VSELs). Asterisks in figure denote statistical significance, (p= 0.0002).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4388887&req=5

Figure 0008: A) Average non-fasting glucose levels of control and experimental diabetic mice. B) Average non-fasting glucose levels of diabetic group, before and after treatment (the mean blood sugar of treated diabetic mice is calculated 3 weeks after cell therapy with VSELs). Asterisks in figure denote statistical significance, (p= 0.0002).
Mentions: Within a week after VSEL administration, blood glucose levels significantly decreased until reaching almost euglycemic values a month later. Hyperglycemia correction lasted at least for 2 months (Figure 8).

Bottom Line: It was proved that CD45-, CXCR4+, and Sca1+ sorted cells express oct4 and SSEA1.Our results revealed that intravenously implanted VSELs could migrate into the pancreas of hosts and survive in the diabetic pancreas.In treated groups, blood glucose decreased significantly for at least two month and the weights of mice increased gradually.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran.

ABSTRACT

Background: Diabetes Mellitus (DM), simply known as diabetes, refers to a group of metabolic diseases in which there are high blood sugar levels over a prolonged period. In this study, the feasibility and safety of intravenous transplantation of Very Small Embryonic Like stem cells (VSELs) were investigated for diabetes repair, and finally the migration and distribution of these cells in hosts were observed.

Methods: Mouse bone marrow VSELs were isolated by Fluorescent Activating Cell Sorting (FACS) method by using fluorescent antibodies against CD45, CXCR4 and Sca1 markers. Sorted cells were analyzed for expression of oct4 and SSEA1 markers with immunocytochemistry staining method. To determine multilineage differentiation, sorted cells were differentiated to Schwann, osteocyte and beta cells. Ten days after the establishment of a mouse model of pancreas necrosis, DiI-labeled VSELs were injected into these mice via tail vein. Pancreases were harvested 4 weeks after transplantation and the sections of these tissues were observed under fluorescent microscope.

Results: It was proved that CD45-, CXCR4+, and Sca1+ sorted cells express oct4 and SSEA1. Our results revealed that intravenously implanted VSELs could migrate into the pancreas of hosts and survive in the diabetic pancreas. In treated groups, blood glucose decreased significantly for at least two month and the weights of mice increased gradually.

Conclusion: This study provides a strategy for using VSELs for curing diabetes and other regenerative diseases, and the strategy is considered an alternative for other stem cell types.

No MeSH data available.


Related in: MedlinePlus