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Intravenous transplantation of very small embryonic like stem cells in treatment of diabetes mellitus.

Abouzaripour M, Ragerdi Kashani I, Pasbakhsh P, Atlasy N - Avicenna J Med Biotechnol (2015 Jan-Mar)

Bottom Line: It was proved that CD45-, CXCR4+, and Sca1+ sorted cells express oct4 and SSEA1.Our results revealed that intravenously implanted VSELs could migrate into the pancreas of hosts and survive in the diabetic pancreas.In treated groups, blood glucose decreased significantly for at least two month and the weights of mice increased gradually.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran.

ABSTRACT

Background: Diabetes Mellitus (DM), simply known as diabetes, refers to a group of metabolic diseases in which there are high blood sugar levels over a prolonged period. In this study, the feasibility and safety of intravenous transplantation of Very Small Embryonic Like stem cells (VSELs) were investigated for diabetes repair, and finally the migration and distribution of these cells in hosts were observed.

Methods: Mouse bone marrow VSELs were isolated by Fluorescent Activating Cell Sorting (FACS) method by using fluorescent antibodies against CD45, CXCR4 and Sca1 markers. Sorted cells were analyzed for expression of oct4 and SSEA1 markers with immunocytochemistry staining method. To determine multilineage differentiation, sorted cells were differentiated to Schwann, osteocyte and beta cells. Ten days after the establishment of a mouse model of pancreas necrosis, DiI-labeled VSELs were injected into these mice via tail vein. Pancreases were harvested 4 weeks after transplantation and the sections of these tissues were observed under fluorescent microscope.

Results: It was proved that CD45-, CXCR4+, and Sca1+ sorted cells express oct4 and SSEA1. Our results revealed that intravenously implanted VSELs could migrate into the pancreas of hosts and survive in the diabetic pancreas. In treated groups, blood glucose decreased significantly for at least two month and the weights of mice increased gradually.

Conclusion: This study provides a strategy for using VSELs for curing diabetes and other regenerative diseases, and the strategy is considered an alternative for other stem cell types.

No MeSH data available.


Related in: MedlinePlus

ICC for Glut2 and PDX1 markers in differentiated beta like cells. A) Glut2 expression was indicated by green fluorescence; B) DAPI nuclear counterstain in blue; C) Merged picture (40×); D) PDX1 was indicated by green fluorescence; E) DAPI nuclear counterstain in blue; F) Merged picture (40×).
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Figure 0005: ICC for Glut2 and PDX1 markers in differentiated beta like cells. A) Glut2 expression was indicated by green fluorescence; B) DAPI nuclear counterstain in blue; C) Merged picture (40×); D) PDX1 was indicated by green fluorescence; E) DAPI nuclear counterstain in blue; F) Merged picture (40×).

Mentions: For this purpose, two antibodies were used against antigens of beta like cells; the first was rabbit polyclonal to PDX1primary antibody (ab47267) and goat polyclonal secondary antibody to rabbit IgG-H&L (FITC) (ab97050) for PDX1 detection. The second was anti-glucose transporter GLUT2 primary antibody (ab- 54460) and goat polyclonal secondary antibody to rabbit IgG-H&L (FITC) (ab97050) for GLUT2 detection. Detailed process of the ICC was similar to ICC for VSEL stem cells described earlier (Figure 5).


Intravenous transplantation of very small embryonic like stem cells in treatment of diabetes mellitus.

Abouzaripour M, Ragerdi Kashani I, Pasbakhsh P, Atlasy N - Avicenna J Med Biotechnol (2015 Jan-Mar)

ICC for Glut2 and PDX1 markers in differentiated beta like cells. A) Glut2 expression was indicated by green fluorescence; B) DAPI nuclear counterstain in blue; C) Merged picture (40×); D) PDX1 was indicated by green fluorescence; E) DAPI nuclear counterstain in blue; F) Merged picture (40×).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4388887&req=5

Figure 0005: ICC for Glut2 and PDX1 markers in differentiated beta like cells. A) Glut2 expression was indicated by green fluorescence; B) DAPI nuclear counterstain in blue; C) Merged picture (40×); D) PDX1 was indicated by green fluorescence; E) DAPI nuclear counterstain in blue; F) Merged picture (40×).
Mentions: For this purpose, two antibodies were used against antigens of beta like cells; the first was rabbit polyclonal to PDX1primary antibody (ab47267) and goat polyclonal secondary antibody to rabbit IgG-H&L (FITC) (ab97050) for PDX1 detection. The second was anti-glucose transporter GLUT2 primary antibody (ab- 54460) and goat polyclonal secondary antibody to rabbit IgG-H&L (FITC) (ab97050) for GLUT2 detection. Detailed process of the ICC was similar to ICC for VSEL stem cells described earlier (Figure 5).

Bottom Line: It was proved that CD45-, CXCR4+, and Sca1+ sorted cells express oct4 and SSEA1.Our results revealed that intravenously implanted VSELs could migrate into the pancreas of hosts and survive in the diabetic pancreas.In treated groups, blood glucose decreased significantly for at least two month and the weights of mice increased gradually.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran.

ABSTRACT

Background: Diabetes Mellitus (DM), simply known as diabetes, refers to a group of metabolic diseases in which there are high blood sugar levels over a prolonged period. In this study, the feasibility and safety of intravenous transplantation of Very Small Embryonic Like stem cells (VSELs) were investigated for diabetes repair, and finally the migration and distribution of these cells in hosts were observed.

Methods: Mouse bone marrow VSELs were isolated by Fluorescent Activating Cell Sorting (FACS) method by using fluorescent antibodies against CD45, CXCR4 and Sca1 markers. Sorted cells were analyzed for expression of oct4 and SSEA1 markers with immunocytochemistry staining method. To determine multilineage differentiation, sorted cells were differentiated to Schwann, osteocyte and beta cells. Ten days after the establishment of a mouse model of pancreas necrosis, DiI-labeled VSELs were injected into these mice via tail vein. Pancreases were harvested 4 weeks after transplantation and the sections of these tissues were observed under fluorescent microscope.

Results: It was proved that CD45-, CXCR4+, and Sca1+ sorted cells express oct4 and SSEA1. Our results revealed that intravenously implanted VSELs could migrate into the pancreas of hosts and survive in the diabetic pancreas. In treated groups, blood glucose decreased significantly for at least two month and the weights of mice increased gradually.

Conclusion: This study provides a strategy for using VSELs for curing diabetes and other regenerative diseases, and the strategy is considered an alternative for other stem cell types.

No MeSH data available.


Related in: MedlinePlus