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Intravenous transplantation of very small embryonic like stem cells in treatment of diabetes mellitus.

Abouzaripour M, Ragerdi Kashani I, Pasbakhsh P, Atlasy N - Avicenna J Med Biotechnol (2015 Jan-Mar)

Bottom Line: It was proved that CD45-, CXCR4+, and Sca1+ sorted cells express oct4 and SSEA1.Our results revealed that intravenously implanted VSELs could migrate into the pancreas of hosts and survive in the diabetic pancreas.In treated groups, blood glucose decreased significantly for at least two month and the weights of mice increased gradually.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran.

ABSTRACT

Background: Diabetes Mellitus (DM), simply known as diabetes, refers to a group of metabolic diseases in which there are high blood sugar levels over a prolonged period. In this study, the feasibility and safety of intravenous transplantation of Very Small Embryonic Like stem cells (VSELs) were investigated for diabetes repair, and finally the migration and distribution of these cells in hosts were observed.

Methods: Mouse bone marrow VSELs were isolated by Fluorescent Activating Cell Sorting (FACS) method by using fluorescent antibodies against CD45, CXCR4 and Sca1 markers. Sorted cells were analyzed for expression of oct4 and SSEA1 markers with immunocytochemistry staining method. To determine multilineage differentiation, sorted cells were differentiated to Schwann, osteocyte and beta cells. Ten days after the establishment of a mouse model of pancreas necrosis, DiI-labeled VSELs were injected into these mice via tail vein. Pancreases were harvested 4 weeks after transplantation and the sections of these tissues were observed under fluorescent microscope.

Results: It was proved that CD45-, CXCR4+, and Sca1+ sorted cells express oct4 and SSEA1. Our results revealed that intravenously implanted VSELs could migrate into the pancreas of hosts and survive in the diabetic pancreas. In treated groups, blood glucose decreased significantly for at least two month and the weights of mice increased gradually.

Conclusion: This study provides a strategy for using VSELs for curing diabetes and other regenerative diseases, and the strategy is considered an alternative for other stem cell types.

No MeSH data available.


Related in: MedlinePlus

Von Kossa staining after osteogenic differentiation: Staining for mineral deposition was performed for osteocyte like cells. This staining was done only for demonstration of osteogenic differentiation (40×).
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Figure 0004: Von Kossa staining after osteogenic differentiation: Staining for mineral deposition was performed for osteocyte like cells. This staining was done only for demonstration of osteogenic differentiation (40×).

Mentions: Osteogenesis was confirmed by means of Von Kossa staining. This technique is for demonstrating deposits of calcium or calcium salt. For Von Kossa silver nitrate staining method, cultures were fixed in cold methanol for 15-20 min. After rinsing, the fixed plates were incubated with 5% silver nitrate solution under UV light using 2 cycles of auto-crosslink (1200 mj ×100) in a UV Stratalinker (Strategene, La Jolla, CA). Mineralized nodules were seen as dark brown to black spots (Figure 4).


Intravenous transplantation of very small embryonic like stem cells in treatment of diabetes mellitus.

Abouzaripour M, Ragerdi Kashani I, Pasbakhsh P, Atlasy N - Avicenna J Med Biotechnol (2015 Jan-Mar)

Von Kossa staining after osteogenic differentiation: Staining for mineral deposition was performed for osteocyte like cells. This staining was done only for demonstration of osteogenic differentiation (40×).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4388887&req=5

Figure 0004: Von Kossa staining after osteogenic differentiation: Staining for mineral deposition was performed for osteocyte like cells. This staining was done only for demonstration of osteogenic differentiation (40×).
Mentions: Osteogenesis was confirmed by means of Von Kossa staining. This technique is for demonstrating deposits of calcium or calcium salt. For Von Kossa silver nitrate staining method, cultures were fixed in cold methanol for 15-20 min. After rinsing, the fixed plates were incubated with 5% silver nitrate solution under UV light using 2 cycles of auto-crosslink (1200 mj ×100) in a UV Stratalinker (Strategene, La Jolla, CA). Mineralized nodules were seen as dark brown to black spots (Figure 4).

Bottom Line: It was proved that CD45-, CXCR4+, and Sca1+ sorted cells express oct4 and SSEA1.Our results revealed that intravenously implanted VSELs could migrate into the pancreas of hosts and survive in the diabetic pancreas.In treated groups, blood glucose decreased significantly for at least two month and the weights of mice increased gradually.

View Article: PubMed Central - PubMed

Affiliation: Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran.

ABSTRACT

Background: Diabetes Mellitus (DM), simply known as diabetes, refers to a group of metabolic diseases in which there are high blood sugar levels over a prolonged period. In this study, the feasibility and safety of intravenous transplantation of Very Small Embryonic Like stem cells (VSELs) were investigated for diabetes repair, and finally the migration and distribution of these cells in hosts were observed.

Methods: Mouse bone marrow VSELs were isolated by Fluorescent Activating Cell Sorting (FACS) method by using fluorescent antibodies against CD45, CXCR4 and Sca1 markers. Sorted cells were analyzed for expression of oct4 and SSEA1 markers with immunocytochemistry staining method. To determine multilineage differentiation, sorted cells were differentiated to Schwann, osteocyte and beta cells. Ten days after the establishment of a mouse model of pancreas necrosis, DiI-labeled VSELs were injected into these mice via tail vein. Pancreases were harvested 4 weeks after transplantation and the sections of these tissues were observed under fluorescent microscope.

Results: It was proved that CD45-, CXCR4+, and Sca1+ sorted cells express oct4 and SSEA1. Our results revealed that intravenously implanted VSELs could migrate into the pancreas of hosts and survive in the diabetic pancreas. In treated groups, blood glucose decreased significantly for at least two month and the weights of mice increased gradually.

Conclusion: This study provides a strategy for using VSELs for curing diabetes and other regenerative diseases, and the strategy is considered an alternative for other stem cell types.

No MeSH data available.


Related in: MedlinePlus