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Effects of Combined Soy Isoflavone Extract and Docetaxel Treatment on Murine 4T1 Breast Tumor Model.

Hejazi E, Nasrollahzadeh J, Fatemi R, Barzegar-Yar Mohamadi L, Saliminejad K, Amiri Z, Kimiagar M, Houshyari M, Tavakoli M, Idali F - Avicenna J Med Biotechnol (2015 Jan-Mar)

Bottom Line: In DOCE+SIE group, diet consumption was significantly higher than DOCE group.The mRNA expression was not significantly different among groups.The results suggest that the combination of soy isoflavone as an adjunct to docetaxel chemotherapy can be effective in improving diet consumption in breast cancer.

View Article: PubMed Central - PubMed

Affiliation: Department of Clinical Nutrition and Dietetics, School of Nutrition Sciences and Food Technology, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

ABSTRACT

Background: Emergence of drug resistance has brought major problems in chemotherapy. Using nutrients in combination with chemotherapy could be beneficial for improvement of sensitivity of tumors to drug resistance. Soybean-derived isoflavones have been suggested as chemopreventive agents for certain types of cancer, particularly breast cancer. In this study, the synergistic effects of soy isoflavone extract in combination with docetaxel in murine 4T1 breast tumor model were investigated.

Methods: In this study, mice were divided into 4 groups (15 mice per group) of control, the dietary Soy Isoflavone Extract (SIE, 100 mg/kg diet), the Docetaxel (DOCE, 10 mg/kg) injection and the combination of dietary soy isoflavone extract and intravenous docetaxel injection (DOCE+SIE). After 3 injections of docetaxel (once a week), 7 mice were sacrificed to analyze MKI67 gene and protein expressions and the rest were monitored for diet consumption, tumor growth and survival rates.

Results: In DOCE+SIE group, diet consumption was significantly higher than DOCE group. While lifespan showed a trend towards improvement in DOCE+SIE group, no significant difference was observed among the 4 studied groups. Tumor volume was not significantly affected in treated groups. A lower but not significant MKI67 protein expression was detected in western blot in DOCE+SIE group. The mRNA expression was not significantly different among groups.

Conclusion: The results suggest that the combination of soy isoflavone as an adjunct to docetaxel chemotherapy can be effective in improving diet consumption in breast cancer.

No MeSH data available.


Related in: MedlinePlus

Survival curves of 4T1 induced breast cancer mouse models in different treatment groups (8 per group). The mean survival times in the control, SIE, DOCE and DOCE+SIE groups were 33.3± 2.7, 34.7±2.1, 38±1.7 and 40.4±2 days, respectively. No significant differences were detected between groups.
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Figure 0002: Survival curves of 4T1 induced breast cancer mouse models in different treatment groups (8 per group). The mean survival times in the control, SIE, DOCE and DOCE+SIE groups were 33.3± 2.7, 34.7±2.1, 38±1.7 and 40.4±2 days, respectively. No significant differences were detected between groups.

Mentions: In survival analysis (Figure 2), the mean survival times in the control, SIE, DOCE and DOCE+SIE groups were 33.3±2.7, 34.7±2.1, 38±1.7 and 40.4±2 days, respectively. While lifespan did not differ significantly between groups (p = 0.34), the DOCE+SIE group had the longest lifespan among the groups.


Effects of Combined Soy Isoflavone Extract and Docetaxel Treatment on Murine 4T1 Breast Tumor Model.

Hejazi E, Nasrollahzadeh J, Fatemi R, Barzegar-Yar Mohamadi L, Saliminejad K, Amiri Z, Kimiagar M, Houshyari M, Tavakoli M, Idali F - Avicenna J Med Biotechnol (2015 Jan-Mar)

Survival curves of 4T1 induced breast cancer mouse models in different treatment groups (8 per group). The mean survival times in the control, SIE, DOCE and DOCE+SIE groups were 33.3± 2.7, 34.7±2.1, 38±1.7 and 40.4±2 days, respectively. No significant differences were detected between groups.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4388885&req=5

Figure 0002: Survival curves of 4T1 induced breast cancer mouse models in different treatment groups (8 per group). The mean survival times in the control, SIE, DOCE and DOCE+SIE groups were 33.3± 2.7, 34.7±2.1, 38±1.7 and 40.4±2 days, respectively. No significant differences were detected between groups.
Mentions: In survival analysis (Figure 2), the mean survival times in the control, SIE, DOCE and DOCE+SIE groups were 33.3±2.7, 34.7±2.1, 38±1.7 and 40.4±2 days, respectively. While lifespan did not differ significantly between groups (p = 0.34), the DOCE+SIE group had the longest lifespan among the groups.

Bottom Line: In DOCE+SIE group, diet consumption was significantly higher than DOCE group.The mRNA expression was not significantly different among groups.The results suggest that the combination of soy isoflavone as an adjunct to docetaxel chemotherapy can be effective in improving diet consumption in breast cancer.

View Article: PubMed Central - PubMed

Affiliation: Department of Clinical Nutrition and Dietetics, School of Nutrition Sciences and Food Technology, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

ABSTRACT

Background: Emergence of drug resistance has brought major problems in chemotherapy. Using nutrients in combination with chemotherapy could be beneficial for improvement of sensitivity of tumors to drug resistance. Soybean-derived isoflavones have been suggested as chemopreventive agents for certain types of cancer, particularly breast cancer. In this study, the synergistic effects of soy isoflavone extract in combination with docetaxel in murine 4T1 breast tumor model were investigated.

Methods: In this study, mice were divided into 4 groups (15 mice per group) of control, the dietary Soy Isoflavone Extract (SIE, 100 mg/kg diet), the Docetaxel (DOCE, 10 mg/kg) injection and the combination of dietary soy isoflavone extract and intravenous docetaxel injection (DOCE+SIE). After 3 injections of docetaxel (once a week), 7 mice were sacrificed to analyze MKI67 gene and protein expressions and the rest were monitored for diet consumption, tumor growth and survival rates.

Results: In DOCE+SIE group, diet consumption was significantly higher than DOCE group. While lifespan showed a trend towards improvement in DOCE+SIE group, no significant difference was observed among the 4 studied groups. Tumor volume was not significantly affected in treated groups. A lower but not significant MKI67 protein expression was detected in western blot in DOCE+SIE group. The mRNA expression was not significantly different among groups.

Conclusion: The results suggest that the combination of soy isoflavone as an adjunct to docetaxel chemotherapy can be effective in improving diet consumption in breast cancer.

No MeSH data available.


Related in: MedlinePlus