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Changes of micro-RNAs in asymptomatic subjects sensitized to Japanese cedar pollen after prophylactic sublingual immunotherapy.

Hou B, Murata M, Said AS, Sakaida H, Masuda S, Takahashi T, Zhang Z, Takeuchi K - Allergy Rhinol (Providence) (2015)

Bottom Line: Serum hsa-miR-223 was significantly up-regulated in postseason compared with preseason samples.A significant decrease in circulating let-7b was also observed in the subjects who developed symptoms.Alterations in miRNA expression occurred in asymptomatic, sensitized subjects during cedar pollen season.

View Article: PubMed Central - PubMed

Affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, Mie University Graduate School of Medicine, Tsu, Japan.

ABSTRACT
Japanese cedar pollinosis is the predominant seasonal allergic rhinitis in Japan, and it has increased in prevalence during the past 10 years. Sublingual immunotherapy (SLIT) is considered a safe and effective treatment for pollinosis. Micro-RNAs (miRNAs) are a class of short single-stranded RNA molecules that posttranscriptionally silence gene expression and may mediate allergic immune responses. The aim of this study was to investigate the miRNA alteration in asymptomatic subjects sensitized to Japanese cedar pollen under prophylactic SLIT under part of a randomized, double-blind, placebo-controlled, multiple-center trial. Analysis was undertaken in 15 asymptomatic subjects sensitized to Japanese cedar pollen-specific IgE (ImmunoCAP class ≥2) who participated in 2013. The SLIT group (n = 6) received standardized Japanese cedar pollen extract and the placebo group (n = 9) received an inactive placebo for 5 months covering the cedar pollen season. Changes in serum miRNAs were measured by real-time quantitative polymerase chain reaction to determine whether SLIT had effects on profiles of circulating miRNA. Seven subjects in the placebo group developed pollinosis symptoms, whereas no subjects in the SLIT group did (p = 0.007). Serum hsa-miR-223 was significantly up-regulated in postseason compared with preseason samples. The hsa-let-7b was significantly more down-regulated in postseason than in preseason samples from the placebo group; however, no significant differences were observed in those from the SLIT group. A significant decrease in circulating let-7b was also observed in the subjects who developed symptoms. Prophylactic SLIT was effective in preventing the development of pollinosis. Alterations in miRNA expression occurred in asymptomatic, sensitized subjects during cedar pollen season.

No MeSH data available.


Related in: MedlinePlus

Comparison of expression levels of let-7b between preseason and postseason among the (A) placebo and (B) sublingual immunotherapy (SLIT) groups. The value of micro-RNA (miRNA) in preseason samples was designated as 1 for each subject, and the fold changes in miRNA values in postseason samples were then determined. Statistical significance was determined with a Student's paired t-test using ΔCt value adjusted with the reference value. Placebo group, closed circles and solid lines; SLIT group, open circles and dotted lines.
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Figure 2: Comparison of expression levels of let-7b between preseason and postseason among the (A) placebo and (B) sublingual immunotherapy (SLIT) groups. The value of micro-RNA (miRNA) in preseason samples was designated as 1 for each subject, and the fold changes in miRNA values in postseason samples were then determined. Statistical significance was determined with a Student's paired t-test using ΔCt value adjusted with the reference value. Placebo group, closed circles and solid lines; SLIT group, open circles and dotted lines.

Mentions: Neither SLIT nor development of pollinosis was significantly associated with a change in any miRNA level other than that of let-7b (data not shown). Interestingly, let-7b levels decreased significantly in the placebo group (Fig. 2A; p = 0.004) but not in the SLIT group (Fig. 2B; p = 0.811). Serum let-7b levels of subjects who had developed pollinosis were significantly lower in the postseason than in the preseason (Fig. 3A; p = 0.007), whereas no significant differences were evident between preseason and postseason in subjects with no symptoms (Fig. 3B; p = 0.935).


Changes of micro-RNAs in asymptomatic subjects sensitized to Japanese cedar pollen after prophylactic sublingual immunotherapy.

Hou B, Murata M, Said AS, Sakaida H, Masuda S, Takahashi T, Zhang Z, Takeuchi K - Allergy Rhinol (Providence) (2015)

Comparison of expression levels of let-7b between preseason and postseason among the (A) placebo and (B) sublingual immunotherapy (SLIT) groups. The value of micro-RNA (miRNA) in preseason samples was designated as 1 for each subject, and the fold changes in miRNA values in postseason samples were then determined. Statistical significance was determined with a Student's paired t-test using ΔCt value adjusted with the reference value. Placebo group, closed circles and solid lines; SLIT group, open circles and dotted lines.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4388874&req=5

Figure 2: Comparison of expression levels of let-7b between preseason and postseason among the (A) placebo and (B) sublingual immunotherapy (SLIT) groups. The value of micro-RNA (miRNA) in preseason samples was designated as 1 for each subject, and the fold changes in miRNA values in postseason samples were then determined. Statistical significance was determined with a Student's paired t-test using ΔCt value adjusted with the reference value. Placebo group, closed circles and solid lines; SLIT group, open circles and dotted lines.
Mentions: Neither SLIT nor development of pollinosis was significantly associated with a change in any miRNA level other than that of let-7b (data not shown). Interestingly, let-7b levels decreased significantly in the placebo group (Fig. 2A; p = 0.004) but not in the SLIT group (Fig. 2B; p = 0.811). Serum let-7b levels of subjects who had developed pollinosis were significantly lower in the postseason than in the preseason (Fig. 3A; p = 0.007), whereas no significant differences were evident between preseason and postseason in subjects with no symptoms (Fig. 3B; p = 0.935).

Bottom Line: Serum hsa-miR-223 was significantly up-regulated in postseason compared with preseason samples.A significant decrease in circulating let-7b was also observed in the subjects who developed symptoms.Alterations in miRNA expression occurred in asymptomatic, sensitized subjects during cedar pollen season.

View Article: PubMed Central - PubMed

Affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, Mie University Graduate School of Medicine, Tsu, Japan.

ABSTRACT
Japanese cedar pollinosis is the predominant seasonal allergic rhinitis in Japan, and it has increased in prevalence during the past 10 years. Sublingual immunotherapy (SLIT) is considered a safe and effective treatment for pollinosis. Micro-RNAs (miRNAs) are a class of short single-stranded RNA molecules that posttranscriptionally silence gene expression and may mediate allergic immune responses. The aim of this study was to investigate the miRNA alteration in asymptomatic subjects sensitized to Japanese cedar pollen under prophylactic SLIT under part of a randomized, double-blind, placebo-controlled, multiple-center trial. Analysis was undertaken in 15 asymptomatic subjects sensitized to Japanese cedar pollen-specific IgE (ImmunoCAP class ≥2) who participated in 2013. The SLIT group (n = 6) received standardized Japanese cedar pollen extract and the placebo group (n = 9) received an inactive placebo for 5 months covering the cedar pollen season. Changes in serum miRNAs were measured by real-time quantitative polymerase chain reaction to determine whether SLIT had effects on profiles of circulating miRNA. Seven subjects in the placebo group developed pollinosis symptoms, whereas no subjects in the SLIT group did (p = 0.007). Serum hsa-miR-223 was significantly up-regulated in postseason compared with preseason samples. The hsa-let-7b was significantly more down-regulated in postseason than in preseason samples from the placebo group; however, no significant differences were observed in those from the SLIT group. A significant decrease in circulating let-7b was also observed in the subjects who developed symptoms. Prophylactic SLIT was effective in preventing the development of pollinosis. Alterations in miRNA expression occurred in asymptomatic, sensitized subjects during cedar pollen season.

No MeSH data available.


Related in: MedlinePlus