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Changes of micro-RNAs in asymptomatic subjects sensitized to Japanese cedar pollen after prophylactic sublingual immunotherapy.

Hou B, Murata M, Said AS, Sakaida H, Masuda S, Takahashi T, Zhang Z, Takeuchi K - Allergy Rhinol (Providence) (2015)

Bottom Line: Serum hsa-miR-223 was significantly up-regulated in postseason compared with preseason samples.A significant decrease in circulating let-7b was also observed in the subjects who developed symptoms.Alterations in miRNA expression occurred in asymptomatic, sensitized subjects during cedar pollen season.

View Article: PubMed Central - PubMed

Affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, Mie University Graduate School of Medicine, Tsu, Japan.

ABSTRACT
Japanese cedar pollinosis is the predominant seasonal allergic rhinitis in Japan, and it has increased in prevalence during the past 10 years. Sublingual immunotherapy (SLIT) is considered a safe and effective treatment for pollinosis. Micro-RNAs (miRNAs) are a class of short single-stranded RNA molecules that posttranscriptionally silence gene expression and may mediate allergic immune responses. The aim of this study was to investigate the miRNA alteration in asymptomatic subjects sensitized to Japanese cedar pollen under prophylactic SLIT under part of a randomized, double-blind, placebo-controlled, multiple-center trial. Analysis was undertaken in 15 asymptomatic subjects sensitized to Japanese cedar pollen-specific IgE (ImmunoCAP class ≥2) who participated in 2013. The SLIT group (n = 6) received standardized Japanese cedar pollen extract and the placebo group (n = 9) received an inactive placebo for 5 months covering the cedar pollen season. Changes in serum miRNAs were measured by real-time quantitative polymerase chain reaction to determine whether SLIT had effects on profiles of circulating miRNA. Seven subjects in the placebo group developed pollinosis symptoms, whereas no subjects in the SLIT group did (p = 0.007). Serum hsa-miR-223 was significantly up-regulated in postseason compared with preseason samples. The hsa-let-7b was significantly more down-regulated in postseason than in preseason samples from the placebo group; however, no significant differences were observed in those from the SLIT group. A significant decrease in circulating let-7b was also observed in the subjects who developed symptoms. Prophylactic SLIT was effective in preventing the development of pollinosis. Alterations in miRNA expression occurred in asymptomatic, sensitized subjects during cedar pollen season.

No MeSH data available.


Related in: MedlinePlus

Change in serum miR-223 expression for each participant. The value of micro-RNA (miRNA) in preseason samples was designated as 1 for each subject, and the fold changes in miRNA value in postseason samples were then determined. Statistical significance was determined with a Student's paired t-test using ΔCt values adjusted with the reference value. Placebo group, closed circles and solid lines; sublingual immunotherapy (SLIT) group, open circles and dotted lines.
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Figure 1: Change in serum miR-223 expression for each participant. The value of micro-RNA (miRNA) in preseason samples was designated as 1 for each subject, and the fold changes in miRNA value in postseason samples were then determined. Statistical significance was determined with a Student's paired t-test using ΔCt values adjusted with the reference value. Placebo group, closed circles and solid lines; sublingual immunotherapy (SLIT) group, open circles and dotted lines.

Mentions: The miR-223 levels were significantly (p = 0.017) higher in postseason than in preseason samples (Fig. 1). However, no significant difference was observed for any other miRNA between preseason and postseason samples (data not shown).


Changes of micro-RNAs in asymptomatic subjects sensitized to Japanese cedar pollen after prophylactic sublingual immunotherapy.

Hou B, Murata M, Said AS, Sakaida H, Masuda S, Takahashi T, Zhang Z, Takeuchi K - Allergy Rhinol (Providence) (2015)

Change in serum miR-223 expression for each participant. The value of micro-RNA (miRNA) in preseason samples was designated as 1 for each subject, and the fold changes in miRNA value in postseason samples were then determined. Statistical significance was determined with a Student's paired t-test using ΔCt values adjusted with the reference value. Placebo group, closed circles and solid lines; sublingual immunotherapy (SLIT) group, open circles and dotted lines.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4388874&req=5

Figure 1: Change in serum miR-223 expression for each participant. The value of micro-RNA (miRNA) in preseason samples was designated as 1 for each subject, and the fold changes in miRNA value in postseason samples were then determined. Statistical significance was determined with a Student's paired t-test using ΔCt values adjusted with the reference value. Placebo group, closed circles and solid lines; sublingual immunotherapy (SLIT) group, open circles and dotted lines.
Mentions: The miR-223 levels were significantly (p = 0.017) higher in postseason than in preseason samples (Fig. 1). However, no significant difference was observed for any other miRNA between preseason and postseason samples (data not shown).

Bottom Line: Serum hsa-miR-223 was significantly up-regulated in postseason compared with preseason samples.A significant decrease in circulating let-7b was also observed in the subjects who developed symptoms.Alterations in miRNA expression occurred in asymptomatic, sensitized subjects during cedar pollen season.

View Article: PubMed Central - PubMed

Affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, Mie University Graduate School of Medicine, Tsu, Japan.

ABSTRACT
Japanese cedar pollinosis is the predominant seasonal allergic rhinitis in Japan, and it has increased in prevalence during the past 10 years. Sublingual immunotherapy (SLIT) is considered a safe and effective treatment for pollinosis. Micro-RNAs (miRNAs) are a class of short single-stranded RNA molecules that posttranscriptionally silence gene expression and may mediate allergic immune responses. The aim of this study was to investigate the miRNA alteration in asymptomatic subjects sensitized to Japanese cedar pollen under prophylactic SLIT under part of a randomized, double-blind, placebo-controlled, multiple-center trial. Analysis was undertaken in 15 asymptomatic subjects sensitized to Japanese cedar pollen-specific IgE (ImmunoCAP class ≥2) who participated in 2013. The SLIT group (n = 6) received standardized Japanese cedar pollen extract and the placebo group (n = 9) received an inactive placebo for 5 months covering the cedar pollen season. Changes in serum miRNAs were measured by real-time quantitative polymerase chain reaction to determine whether SLIT had effects on profiles of circulating miRNA. Seven subjects in the placebo group developed pollinosis symptoms, whereas no subjects in the SLIT group did (p = 0.007). Serum hsa-miR-223 was significantly up-regulated in postseason compared with preseason samples. The hsa-let-7b was significantly more down-regulated in postseason than in preseason samples from the placebo group; however, no significant differences were observed in those from the SLIT group. A significant decrease in circulating let-7b was also observed in the subjects who developed symptoms. Prophylactic SLIT was effective in preventing the development of pollinosis. Alterations in miRNA expression occurred in asymptomatic, sensitized subjects during cedar pollen season.

No MeSH data available.


Related in: MedlinePlus