Limits...
Mitogen-activated protein kinases are associated with the regulation of physiological traits and virulence in Fusarium oxysporum f. sp. cubense.

Ding Z, Li M, Sun F, Xi P, Sun L, Zhang L, Jiang Z - PLoS ONE (2015)

Bottom Line: Transcriptional analysis revealed that the MAP kinase signaling pathway plays a key role in regulation of the genes encoding production of chitin, peroxidase, beauvericin and fusaric acid.Moreover, disruption of the MAP kinase genes resulted in abnormal hypha and increased sensitivity to Congo Red, Calcofluor White and H2O2.Taken together, these results depict the critical roles of MAP kinases in regulation of FOC physiology and virulence.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Pathology, South China Agricultural University, Guangzhou 510642, China; Guangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, Guangzhou 510642, China.

ABSTRACT
Fusarium oxysporum f. sp. cubense (FOC) is an important soil-borne fungal pathogen causing devastating vascular wilt disease of banana plants and has become a great concern threatening banana production worldwide. However, little information is known about the molecular mechanisms that govern the expression of virulence determinants of this important fungal pathogen. In this study, we showed that mutation of three mitogen-activated protein (MAP) kinase genes, designated as FoSlt2, FoMkk2 and FoBck1, respectively, led to substantial attenuation in fungal virulence on banana plants. Transcriptional analysis revealed that the MAP kinase signaling pathway plays a key role in regulation of the genes encoding production of chitin, peroxidase, beauvericin and fusaric acid. Biochemical analysis further confirmed the essential role of MAP kinases in modulating the production of fusaric acid, which was a crucial phytotoxin in accelerating development of Fusarium wilt symptoms in banana plants. Additionally, we found that the MAP kinase FoSlt2 was required for siderophore biosynthesis under iron-depletion conditions. Moreover, disruption of the MAP kinase genes resulted in abnormal hypha and increased sensitivity to Congo Red, Calcofluor White and H2O2. Taken together, these results depict the critical roles of MAP kinases in regulation of FOC physiology and virulence.

No MeSH data available.


Related in: MedlinePlus

Fusaric acid (FA) production was reduced in mutants ΔFoSlt2, ΔFoMkk2 and ΔFoBck1.(A) Fusaric acid production from cultures of the indicated strains in Czapek dox medium was analysed by High Performance Liquid Chromatography (HPLC). Fusaric acid levels are represented in μg g-1 mycelial dry weight. (B) Fusaric acid production from the crude mycotoxin extracted of the indicated strains in grains cultures was analysed with HPLC. Fusaric acid levels are represented in μg g-1 grains cultures dry weight. Error bars indicate the standard error from three replicates.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4388850&req=5

pone.0122634.g008: Fusaric acid (FA) production was reduced in mutants ΔFoSlt2, ΔFoMkk2 and ΔFoBck1.(A) Fusaric acid production from cultures of the indicated strains in Czapek dox medium was analysed by High Performance Liquid Chromatography (HPLC). Fusaric acid levels are represented in μg g-1 mycelial dry weight. (B) Fusaric acid production from the crude mycotoxin extracted of the indicated strains in grains cultures was analysed with HPLC. Fusaric acid levels are represented in μg g-1 grains cultures dry weight. Error bars indicate the standard error from three replicates.

Mentions: We then determined the fusaric acid production in Czapek dox medium by WT and mutants by high performance liquid chromatography (HPLC). In agreement with the gene expression data, the production of fusaric acid was reduced in mutants ΔFoSlt2, ΔFoMkk2 and ΔFoBck1 compared with that of WT, respectively (Fig 8A).


Mitogen-activated protein kinases are associated with the regulation of physiological traits and virulence in Fusarium oxysporum f. sp. cubense.

Ding Z, Li M, Sun F, Xi P, Sun L, Zhang L, Jiang Z - PLoS ONE (2015)

Fusaric acid (FA) production was reduced in mutants ΔFoSlt2, ΔFoMkk2 and ΔFoBck1.(A) Fusaric acid production from cultures of the indicated strains in Czapek dox medium was analysed by High Performance Liquid Chromatography (HPLC). Fusaric acid levels are represented in μg g-1 mycelial dry weight. (B) Fusaric acid production from the crude mycotoxin extracted of the indicated strains in grains cultures was analysed with HPLC. Fusaric acid levels are represented in μg g-1 grains cultures dry weight. Error bars indicate the standard error from three replicates.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4388850&req=5

pone.0122634.g008: Fusaric acid (FA) production was reduced in mutants ΔFoSlt2, ΔFoMkk2 and ΔFoBck1.(A) Fusaric acid production from cultures of the indicated strains in Czapek dox medium was analysed by High Performance Liquid Chromatography (HPLC). Fusaric acid levels are represented in μg g-1 mycelial dry weight. (B) Fusaric acid production from the crude mycotoxin extracted of the indicated strains in grains cultures was analysed with HPLC. Fusaric acid levels are represented in μg g-1 grains cultures dry weight. Error bars indicate the standard error from three replicates.
Mentions: We then determined the fusaric acid production in Czapek dox medium by WT and mutants by high performance liquid chromatography (HPLC). In agreement with the gene expression data, the production of fusaric acid was reduced in mutants ΔFoSlt2, ΔFoMkk2 and ΔFoBck1 compared with that of WT, respectively (Fig 8A).

Bottom Line: Transcriptional analysis revealed that the MAP kinase signaling pathway plays a key role in regulation of the genes encoding production of chitin, peroxidase, beauvericin and fusaric acid.Moreover, disruption of the MAP kinase genes resulted in abnormal hypha and increased sensitivity to Congo Red, Calcofluor White and H2O2.Taken together, these results depict the critical roles of MAP kinases in regulation of FOC physiology and virulence.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Pathology, South China Agricultural University, Guangzhou 510642, China; Guangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, Guangzhou 510642, China.

ABSTRACT
Fusarium oxysporum f. sp. cubense (FOC) is an important soil-borne fungal pathogen causing devastating vascular wilt disease of banana plants and has become a great concern threatening banana production worldwide. However, little information is known about the molecular mechanisms that govern the expression of virulence determinants of this important fungal pathogen. In this study, we showed that mutation of three mitogen-activated protein (MAP) kinase genes, designated as FoSlt2, FoMkk2 and FoBck1, respectively, led to substantial attenuation in fungal virulence on banana plants. Transcriptional analysis revealed that the MAP kinase signaling pathway plays a key role in regulation of the genes encoding production of chitin, peroxidase, beauvericin and fusaric acid. Biochemical analysis further confirmed the essential role of MAP kinases in modulating the production of fusaric acid, which was a crucial phytotoxin in accelerating development of Fusarium wilt symptoms in banana plants. Additionally, we found that the MAP kinase FoSlt2 was required for siderophore biosynthesis under iron-depletion conditions. Moreover, disruption of the MAP kinase genes resulted in abnormal hypha and increased sensitivity to Congo Red, Calcofluor White and H2O2. Taken together, these results depict the critical roles of MAP kinases in regulation of FOC physiology and virulence.

No MeSH data available.


Related in: MedlinePlus