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Mitogen-activated protein kinases are associated with the regulation of physiological traits and virulence in Fusarium oxysporum f. sp. cubense.

Ding Z, Li M, Sun F, Xi P, Sun L, Zhang L, Jiang Z - PLoS ONE (2015)

Bottom Line: Transcriptional analysis revealed that the MAP kinase signaling pathway plays a key role in regulation of the genes encoding production of chitin, peroxidase, beauvericin and fusaric acid.Moreover, disruption of the MAP kinase genes resulted in abnormal hypha and increased sensitivity to Congo Red, Calcofluor White and H2O2.Taken together, these results depict the critical roles of MAP kinases in regulation of FOC physiology and virulence.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Pathology, South China Agricultural University, Guangzhou 510642, China; Guangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, Guangzhou 510642, China.

ABSTRACT
Fusarium oxysporum f. sp. cubense (FOC) is an important soil-borne fungal pathogen causing devastating vascular wilt disease of banana plants and has become a great concern threatening banana production worldwide. However, little information is known about the molecular mechanisms that govern the expression of virulence determinants of this important fungal pathogen. In this study, we showed that mutation of three mitogen-activated protein (MAP) kinase genes, designated as FoSlt2, FoMkk2 and FoBck1, respectively, led to substantial attenuation in fungal virulence on banana plants. Transcriptional analysis revealed that the MAP kinase signaling pathway plays a key role in regulation of the genes encoding production of chitin, peroxidase, beauvericin and fusaric acid. Biochemical analysis further confirmed the essential role of MAP kinases in modulating the production of fusaric acid, which was a crucial phytotoxin in accelerating development of Fusarium wilt symptoms in banana plants. Additionally, we found that the MAP kinase FoSlt2 was required for siderophore biosynthesis under iron-depletion conditions. Moreover, disruption of the MAP kinase genes resulted in abnormal hypha and increased sensitivity to Congo Red, Calcofluor White and H2O2. Taken together, these results depict the critical roles of MAP kinases in regulation of FOC physiology and virulence.

No MeSH data available.


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The MAP kinases regulate transcription of the fusaric acid gene cluster.Gene expression levels are represented relative to WT using quantitative real-time PCR. Error bars indicate the standard error from three replicates.
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pone.0122634.g007: The MAP kinases regulate transcription of the fusaric acid gene cluster.Gene expression levels are represented relative to WT using quantitative real-time PCR. Error bars indicate the standard error from three replicates.

Mentions: To determine the involvement of MAP kinases in the regulation of fusaric acid biosynthesis, we analyzed the expression levels of five genes involved in fusaric acid biosynthesis. The expression levels of fusaric acid biosynthetic genes (FUB1 to FUB5) were significantly reduced in mutants ΔFoSlt2 (100-fold, 11-fold, 10-fold, 25-fold and 50-fold, respectively), ΔFoMkk2 (100-fold, 13-fold, 10-fold, 20-fold and 33-fold, respectively) and ΔFoBck1 (50-fold, 6-fold, 6-fold, 17-fold and 25-fold, respectively) compared with that of WT, respectively. In the complemented strains ΔFoSlt2-c and ΔFoMkk2-c, expression levels of the five genes were completely or partially restored to the WT levels, respectively (Fig 7).


Mitogen-activated protein kinases are associated with the regulation of physiological traits and virulence in Fusarium oxysporum f. sp. cubense.

Ding Z, Li M, Sun F, Xi P, Sun L, Zhang L, Jiang Z - PLoS ONE (2015)

The MAP kinases regulate transcription of the fusaric acid gene cluster.Gene expression levels are represented relative to WT using quantitative real-time PCR. Error bars indicate the standard error from three replicates.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4388850&req=5

pone.0122634.g007: The MAP kinases regulate transcription of the fusaric acid gene cluster.Gene expression levels are represented relative to WT using quantitative real-time PCR. Error bars indicate the standard error from three replicates.
Mentions: To determine the involvement of MAP kinases in the regulation of fusaric acid biosynthesis, we analyzed the expression levels of five genes involved in fusaric acid biosynthesis. The expression levels of fusaric acid biosynthetic genes (FUB1 to FUB5) were significantly reduced in mutants ΔFoSlt2 (100-fold, 11-fold, 10-fold, 25-fold and 50-fold, respectively), ΔFoMkk2 (100-fold, 13-fold, 10-fold, 20-fold and 33-fold, respectively) and ΔFoBck1 (50-fold, 6-fold, 6-fold, 17-fold and 25-fold, respectively) compared with that of WT, respectively. In the complemented strains ΔFoSlt2-c and ΔFoMkk2-c, expression levels of the five genes were completely or partially restored to the WT levels, respectively (Fig 7).

Bottom Line: Transcriptional analysis revealed that the MAP kinase signaling pathway plays a key role in regulation of the genes encoding production of chitin, peroxidase, beauvericin and fusaric acid.Moreover, disruption of the MAP kinase genes resulted in abnormal hypha and increased sensitivity to Congo Red, Calcofluor White and H2O2.Taken together, these results depict the critical roles of MAP kinases in regulation of FOC physiology and virulence.

View Article: PubMed Central - PubMed

Affiliation: Department of Plant Pathology, South China Agricultural University, Guangzhou 510642, China; Guangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, Guangzhou 510642, China.

ABSTRACT
Fusarium oxysporum f. sp. cubense (FOC) is an important soil-borne fungal pathogen causing devastating vascular wilt disease of banana plants and has become a great concern threatening banana production worldwide. However, little information is known about the molecular mechanisms that govern the expression of virulence determinants of this important fungal pathogen. In this study, we showed that mutation of three mitogen-activated protein (MAP) kinase genes, designated as FoSlt2, FoMkk2 and FoBck1, respectively, led to substantial attenuation in fungal virulence on banana plants. Transcriptional analysis revealed that the MAP kinase signaling pathway plays a key role in regulation of the genes encoding production of chitin, peroxidase, beauvericin and fusaric acid. Biochemical analysis further confirmed the essential role of MAP kinases in modulating the production of fusaric acid, which was a crucial phytotoxin in accelerating development of Fusarium wilt symptoms in banana plants. Additionally, we found that the MAP kinase FoSlt2 was required for siderophore biosynthesis under iron-depletion conditions. Moreover, disruption of the MAP kinase genes resulted in abnormal hypha and increased sensitivity to Congo Red, Calcofluor White and H2O2. Taken together, these results depict the critical roles of MAP kinases in regulation of FOC physiology and virulence.

No MeSH data available.


Related in: MedlinePlus