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Fusarium oxysporum triggers tissue-specific transcriptional reprogramming in Arabidopsis thaliana.

Lyons R, Stiller J, Powell J, Rusu A, Manners JM, Kazan K - PLoS ONE (2015)

Bottom Line: At least half of the genes induced or repressed by F. oxysporum showed tissue-specific regulation.Regulators of auxin and ABA signalling, mannose binding lectins and peroxidases showed strong differential expression in root tissue.We demonstrate that ARF2 and PRX33, two genes regulated in the roots, promote susceptibility to F. oxysporum.

View Article: PubMed Central - PubMed

Affiliation: CSIRO Agriculture Flagship, Queensland Bioscience Precinct, Brisbane, QLD, Australia.

ABSTRACT
Some of the most devastating agricultural diseases are caused by root-infecting pathogens, yet the majority of studies on these interactions to date have focused on the host responses of aerial tissues rather than those belowground. Fusarium oxysporum is a root-infecting pathogen that causes wilt disease on several plant species including Arabidopsis thaliana. To investigate and compare transcriptional changes triggered by F. oxysporum in different Arabidopsis tissues, we infected soil-grown plants with F. oxysporum and subjected root and leaf tissue harvested at early and late timepoints to RNA-seq analyses. At least half of the genes induced or repressed by F. oxysporum showed tissue-specific regulation. Regulators of auxin and ABA signalling, mannose binding lectins and peroxidases showed strong differential expression in root tissue. We demonstrate that ARF2 and PRX33, two genes regulated in the roots, promote susceptibility to F. oxysporum. In the leaves, defensins and genes associated with the response to auxin, cold and senescence were strongly regulated while jasmonate biosynthesis and signalling genes were induced throughout the plant.

No MeSH data available.


Related in: MedlinePlus

Genes induced or repressed in different tissues at early and late timepoints after F. oxysporum infection.(A) The number of genes induced (up) or repressed (down) by F. oxysporum in roots and leaves at 1 or 6 dpi. Black bars represent genes regulated <2 fold; grey bars represent genes regulated > 2 fold. (B) Overlap in F. oxysporum—responsive genes between two timepoints, 1 (F1/M1) and 6 dpi (F6/M6). (C) Overlap in F. oxysporum—responsive genes between roots and leaves.
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pone.0121902.g002: Genes induced or repressed in different tissues at early and late timepoints after F. oxysporum infection.(A) The number of genes induced (up) or repressed (down) by F. oxysporum in roots and leaves at 1 or 6 dpi. Black bars represent genes regulated <2 fold; grey bars represent genes regulated > 2 fold. (B) Overlap in F. oxysporum—responsive genes between two timepoints, 1 (F1/M1) and 6 dpi (F6/M6). (C) Overlap in F. oxysporum—responsive genes between roots and leaves.

Mentions: For differential expression analysis of RNAseq data, we used the cuffdiff analysis tool and found substantial numbers of genes, ranging from 417 to 4089, were regulated by F. oxysporum depending on the timepoint, in roots and leaves. Within each timepoint, similar numbers of genes were repressed as were induced. More genes were regulated in the leaves than in the roots at both timepoints and substantially more genes were regulated at 6 dpi relative to 1 dpi (Fig 2A).


Fusarium oxysporum triggers tissue-specific transcriptional reprogramming in Arabidopsis thaliana.

Lyons R, Stiller J, Powell J, Rusu A, Manners JM, Kazan K - PLoS ONE (2015)

Genes induced or repressed in different tissues at early and late timepoints after F. oxysporum infection.(A) The number of genes induced (up) or repressed (down) by F. oxysporum in roots and leaves at 1 or 6 dpi. Black bars represent genes regulated <2 fold; grey bars represent genes regulated > 2 fold. (B) Overlap in F. oxysporum—responsive genes between two timepoints, 1 (F1/M1) and 6 dpi (F6/M6). (C) Overlap in F. oxysporum—responsive genes between roots and leaves.
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Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4388846&req=5

pone.0121902.g002: Genes induced or repressed in different tissues at early and late timepoints after F. oxysporum infection.(A) The number of genes induced (up) or repressed (down) by F. oxysporum in roots and leaves at 1 or 6 dpi. Black bars represent genes regulated <2 fold; grey bars represent genes regulated > 2 fold. (B) Overlap in F. oxysporum—responsive genes between two timepoints, 1 (F1/M1) and 6 dpi (F6/M6). (C) Overlap in F. oxysporum—responsive genes between roots and leaves.
Mentions: For differential expression analysis of RNAseq data, we used the cuffdiff analysis tool and found substantial numbers of genes, ranging from 417 to 4089, were regulated by F. oxysporum depending on the timepoint, in roots and leaves. Within each timepoint, similar numbers of genes were repressed as were induced. More genes were regulated in the leaves than in the roots at both timepoints and substantially more genes were regulated at 6 dpi relative to 1 dpi (Fig 2A).

Bottom Line: At least half of the genes induced or repressed by F. oxysporum showed tissue-specific regulation.Regulators of auxin and ABA signalling, mannose binding lectins and peroxidases showed strong differential expression in root tissue.We demonstrate that ARF2 and PRX33, two genes regulated in the roots, promote susceptibility to F. oxysporum.

View Article: PubMed Central - PubMed

Affiliation: CSIRO Agriculture Flagship, Queensland Bioscience Precinct, Brisbane, QLD, Australia.

ABSTRACT
Some of the most devastating agricultural diseases are caused by root-infecting pathogens, yet the majority of studies on these interactions to date have focused on the host responses of aerial tissues rather than those belowground. Fusarium oxysporum is a root-infecting pathogen that causes wilt disease on several plant species including Arabidopsis thaliana. To investigate and compare transcriptional changes triggered by F. oxysporum in different Arabidopsis tissues, we infected soil-grown plants with F. oxysporum and subjected root and leaf tissue harvested at early and late timepoints to RNA-seq analyses. At least half of the genes induced or repressed by F. oxysporum showed tissue-specific regulation. Regulators of auxin and ABA signalling, mannose binding lectins and peroxidases showed strong differential expression in root tissue. We demonstrate that ARF2 and PRX33, two genes regulated in the roots, promote susceptibility to F. oxysporum. In the leaves, defensins and genes associated with the response to auxin, cold and senescence were strongly regulated while jasmonate biosynthesis and signalling genes were induced throughout the plant.

No MeSH data available.


Related in: MedlinePlus