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Fusarium oxysporum triggers tissue-specific transcriptional reprogramming in Arabidopsis thaliana.

Lyons R, Stiller J, Powell J, Rusu A, Manners JM, Kazan K - PLoS ONE (2015)

Bottom Line: At least half of the genes induced or repressed by F. oxysporum showed tissue-specific regulation.Regulators of auxin and ABA signalling, mannose binding lectins and peroxidases showed strong differential expression in root tissue.We demonstrate that ARF2 and PRX33, two genes regulated in the roots, promote susceptibility to F. oxysporum.

View Article: PubMed Central - PubMed

Affiliation: CSIRO Agriculture Flagship, Queensland Bioscience Precinct, Brisbane, QLD, Australia.

ABSTRACT
Some of the most devastating agricultural diseases are caused by root-infecting pathogens, yet the majority of studies on these interactions to date have focused on the host responses of aerial tissues rather than those belowground. Fusarium oxysporum is a root-infecting pathogen that causes wilt disease on several plant species including Arabidopsis thaliana. To investigate and compare transcriptional changes triggered by F. oxysporum in different Arabidopsis tissues, we infected soil-grown plants with F. oxysporum and subjected root and leaf tissue harvested at early and late timepoints to RNA-seq analyses. At least half of the genes induced or repressed by F. oxysporum showed tissue-specific regulation. Regulators of auxin and ABA signalling, mannose binding lectins and peroxidases showed strong differential expression in root tissue. We demonstrate that ARF2 and PRX33, two genes regulated in the roots, promote susceptibility to F. oxysporum. In the leaves, defensins and genes associated with the response to auxin, cold and senescence were strongly regulated while jasmonate biosynthesis and signalling genes were induced throughout the plant.

No MeSH data available.


Related in: MedlinePlus

Disease symptoms and detection of F. oxysporum RNA in A. thaliana plants after F. oxysporum infection.(A) Representative plants were uprooted and photographed at 1, 6 or 14 days after F. oxysporum or mock inoculation. (B) Relative expression of F. oxysporum β-tubulin normalised to A. thaliana actin in root (black) and leaf (grey) tissue. Data show mean relative expression and standard error from 3 biological replicates containing pools of 10 plants.
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pone.0121902.g001: Disease symptoms and detection of F. oxysporum RNA in A. thaliana plants after F. oxysporum infection.(A) Representative plants were uprooted and photographed at 1, 6 or 14 days after F. oxysporum or mock inoculation. (B) Relative expression of F. oxysporum β-tubulin normalised to A. thaliana actin in root (black) and leaf (grey) tissue. Data show mean relative expression and standard error from 3 biological replicates containing pools of 10 plants.

Mentions: To compare the response of A. thaliana roots and leaves to F. oxysporum infection, we performed RNA-seq experiments and identified genes significantly induced or repressed by F. oxysporum in either tissue at 1 or 6 days post inoculation (dpi). We used 1 dpi as an early timepoint when F. oxysporum is presumably in a biotrophic infection phase and 6 dpi when F. oxysporum is undergoing the switch from a biotrophic to necrotrophic lifestyle. At 1 dpi, F. oxysporum—inoculated plants were indistinguishable from mock—inoculated controls and F. oxysporum RNA was detectable in the roots but not in the leaves. By 6 dpi, classic disease symptoms including vein yellowing and chlorosis appeared on the leaves of Col-0 plants and fungal DNA and RNA was detectable in both root and leaf tissue (Fig 1).


Fusarium oxysporum triggers tissue-specific transcriptional reprogramming in Arabidopsis thaliana.

Lyons R, Stiller J, Powell J, Rusu A, Manners JM, Kazan K - PLoS ONE (2015)

Disease symptoms and detection of F. oxysporum RNA in A. thaliana plants after F. oxysporum infection.(A) Representative plants were uprooted and photographed at 1, 6 or 14 days after F. oxysporum or mock inoculation. (B) Relative expression of F. oxysporum β-tubulin normalised to A. thaliana actin in root (black) and leaf (grey) tissue. Data show mean relative expression and standard error from 3 biological replicates containing pools of 10 plants.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4388846&req=5

pone.0121902.g001: Disease symptoms and detection of F. oxysporum RNA in A. thaliana plants after F. oxysporum infection.(A) Representative plants were uprooted and photographed at 1, 6 or 14 days after F. oxysporum or mock inoculation. (B) Relative expression of F. oxysporum β-tubulin normalised to A. thaliana actin in root (black) and leaf (grey) tissue. Data show mean relative expression and standard error from 3 biological replicates containing pools of 10 plants.
Mentions: To compare the response of A. thaliana roots and leaves to F. oxysporum infection, we performed RNA-seq experiments and identified genes significantly induced or repressed by F. oxysporum in either tissue at 1 or 6 days post inoculation (dpi). We used 1 dpi as an early timepoint when F. oxysporum is presumably in a biotrophic infection phase and 6 dpi when F. oxysporum is undergoing the switch from a biotrophic to necrotrophic lifestyle. At 1 dpi, F. oxysporum—inoculated plants were indistinguishable from mock—inoculated controls and F. oxysporum RNA was detectable in the roots but not in the leaves. By 6 dpi, classic disease symptoms including vein yellowing and chlorosis appeared on the leaves of Col-0 plants and fungal DNA and RNA was detectable in both root and leaf tissue (Fig 1).

Bottom Line: At least half of the genes induced or repressed by F. oxysporum showed tissue-specific regulation.Regulators of auxin and ABA signalling, mannose binding lectins and peroxidases showed strong differential expression in root tissue.We demonstrate that ARF2 and PRX33, two genes regulated in the roots, promote susceptibility to F. oxysporum.

View Article: PubMed Central - PubMed

Affiliation: CSIRO Agriculture Flagship, Queensland Bioscience Precinct, Brisbane, QLD, Australia.

ABSTRACT
Some of the most devastating agricultural diseases are caused by root-infecting pathogens, yet the majority of studies on these interactions to date have focused on the host responses of aerial tissues rather than those belowground. Fusarium oxysporum is a root-infecting pathogen that causes wilt disease on several plant species including Arabidopsis thaliana. To investigate and compare transcriptional changes triggered by F. oxysporum in different Arabidopsis tissues, we infected soil-grown plants with F. oxysporum and subjected root and leaf tissue harvested at early and late timepoints to RNA-seq analyses. At least half of the genes induced or repressed by F. oxysporum showed tissue-specific regulation. Regulators of auxin and ABA signalling, mannose binding lectins and peroxidases showed strong differential expression in root tissue. We demonstrate that ARF2 and PRX33, two genes regulated in the roots, promote susceptibility to F. oxysporum. In the leaves, defensins and genes associated with the response to auxin, cold and senescence were strongly regulated while jasmonate biosynthesis and signalling genes were induced throughout the plant.

No MeSH data available.


Related in: MedlinePlus