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Dynamic changes in amino acid concentration profiles in patients with sepsis.

Su L, Li H, Xie A, Liu D, Rao W, Lan L, Li X, Li F, Xiao K, Wang H, Yan P, Li X, Xie L - PLoS ONE (2015)

Bottom Line: As the disease progressed further or with poor prognosis, the levels of the different amino acids gradually increased, decreased, or fluctuated over time.The serum concentrations of SAAs, especially taurine, exhibited weak negative correlations with the Sequential Organ Failure Assessment (SOFA) (r=-0.319) and Acute Physiology and Chronic Health Evaluation (APACHE) II (r=-0.325) scores.The areas under the receiver operating characteristic curves of cystine, taurine, and SAA levels and the SOFA and APACHE II scores, which denoted disease prognosis, were 0.623, 0.674, 0.678, 0.86, and 0.857, respectively.

View Article: PubMed Central - PubMed

Affiliation: Department of Respiratory Diseases, Chinese PLA General Hospital, Beijing, China; Department of Critical Care Medicine, Peking Union Medical College Hospital, Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing, China.

ABSTRACT

Objectives: The goal of this work was to explore the dynamic concentration profiles of 42 amino acids and the significance of these profiles in relation to sepsis, with the aim of providing guidance for clinical therapies.

Methods: Thirty-five critically ill patients with sepsis were included. These patients were further divided into sepsis (12 cases) and severe sepsis (23 cases) groups or survivor (20 cases) and non-survivor (15 cases) groups. Serum samples from the patients were collected on days 1, 3, 5, 7, 10, and 14 following intensive care unit (ICU) admission, and the serum concentrations of 42 amino acids were measured.

Results: The metabolic spectrum of the amino acids changed dramatically in patients with sepsis. As the disease progressed further or with poor prognosis, the levels of the different amino acids gradually increased, decreased, or fluctuated over time. The concentrations of sulfur-containing amino acids (SAAs), especially taurine, decreased significantly as the severity of sepsis worsened or with poor prognosis of the patient. The serum concentrations of SAAs, especially taurine, exhibited weak negative correlations with the Sequential Organ Failure Assessment (SOFA) (r=-0.319) and Acute Physiology and Chronic Health Evaluation (APACHE) II (r=-0.325) scores. The areas under the receiver operating characteristic curves of cystine, taurine, and SAA levels and the SOFA and APACHE II scores, which denoted disease prognosis, were 0.623, 0.674, 0.678, 0.86, and 0.857, respectively.

Conclusions: Critically ill patients with disorders of amino acid metabolism, especially of SAAs such as cystine and taurine, may provide an indicator of the need for the nutritional support of sepsis in the clinic.

Trial registration: ClinicalTrial.gov identifier NCT01818830.

No MeSH data available.


Related in: MedlinePlus

Trial profiles of the patients enrolled in our study.A total of 55 out of 334 septic patients were selected based on the relevant criteria. The sepsis group was further divided into sepsis (n = 12) and severe sepsis (n = 23) subgroups, as well as into survivor (n = 20) and non-survivor (n = 15) subgroups. Additionally, 18 normal controls and 14 SIRS were also included in this study.
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pone.0121933.g001: Trial profiles of the patients enrolled in our study.A total of 55 out of 334 septic patients were selected based on the relevant criteria. The sepsis group was further divided into sepsis (n = 12) and severe sepsis (n = 23) subgroups, as well as into survivor (n = 20) and non-survivor (n = 15) subgroups. Additionally, 18 normal controls and 14 SIRS were also included in this study.

Mentions: A total of 14 patients with SIRS, 35 patients with sepsis, and 18 normal controls were selected, in accordance with the relevant criteria, to be formally included in this study (Fig. 1). The sepsis group was further divided into sepsis and severe sepsis subgroups, as well as into survivor and non-survivor subgroups. Table 1 provides a general summary of the subjects’ clinical characteristics. The serum procalcitonin (PCT) and blood urea nitrogen (BUN) levels and the APACHE II scores were higher in the sepsis and severe sepsis groups than those in the SIRS and sepsis groups, respectively (P<0.05). Compared with the sepsis group, the SOFA scores in the severe sepsis group were significantly higher (P = 0.001). Regarding the 28-day survivals, the mortality rates for the severe sepsis and sepsis groups were higher than those of the sepsis and SIRS groups, respectively (P<0.05). Additionally, the non-survivor group had higher APACHE II and SOFA scores than the survivor group (P<0.05). The non-survivor group had higher percentages of mechanical ventilation and post-operative trauma (P<0.05). There were no statistically significant differences for any other parameters.


Dynamic changes in amino acid concentration profiles in patients with sepsis.

Su L, Li H, Xie A, Liu D, Rao W, Lan L, Li X, Li F, Xiao K, Wang H, Yan P, Li X, Xie L - PLoS ONE (2015)

Trial profiles of the patients enrolled in our study.A total of 55 out of 334 septic patients were selected based on the relevant criteria. The sepsis group was further divided into sepsis (n = 12) and severe sepsis (n = 23) subgroups, as well as into survivor (n = 20) and non-survivor (n = 15) subgroups. Additionally, 18 normal controls and 14 SIRS were also included in this study.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4388841&req=5

pone.0121933.g001: Trial profiles of the patients enrolled in our study.A total of 55 out of 334 septic patients were selected based on the relevant criteria. The sepsis group was further divided into sepsis (n = 12) and severe sepsis (n = 23) subgroups, as well as into survivor (n = 20) and non-survivor (n = 15) subgroups. Additionally, 18 normal controls and 14 SIRS were also included in this study.
Mentions: A total of 14 patients with SIRS, 35 patients with sepsis, and 18 normal controls were selected, in accordance with the relevant criteria, to be formally included in this study (Fig. 1). The sepsis group was further divided into sepsis and severe sepsis subgroups, as well as into survivor and non-survivor subgroups. Table 1 provides a general summary of the subjects’ clinical characteristics. The serum procalcitonin (PCT) and blood urea nitrogen (BUN) levels and the APACHE II scores were higher in the sepsis and severe sepsis groups than those in the SIRS and sepsis groups, respectively (P<0.05). Compared with the sepsis group, the SOFA scores in the severe sepsis group were significantly higher (P = 0.001). Regarding the 28-day survivals, the mortality rates for the severe sepsis and sepsis groups were higher than those of the sepsis and SIRS groups, respectively (P<0.05). Additionally, the non-survivor group had higher APACHE II and SOFA scores than the survivor group (P<0.05). The non-survivor group had higher percentages of mechanical ventilation and post-operative trauma (P<0.05). There were no statistically significant differences for any other parameters.

Bottom Line: As the disease progressed further or with poor prognosis, the levels of the different amino acids gradually increased, decreased, or fluctuated over time.The serum concentrations of SAAs, especially taurine, exhibited weak negative correlations with the Sequential Organ Failure Assessment (SOFA) (r=-0.319) and Acute Physiology and Chronic Health Evaluation (APACHE) II (r=-0.325) scores.The areas under the receiver operating characteristic curves of cystine, taurine, and SAA levels and the SOFA and APACHE II scores, which denoted disease prognosis, were 0.623, 0.674, 0.678, 0.86, and 0.857, respectively.

View Article: PubMed Central - PubMed

Affiliation: Department of Respiratory Diseases, Chinese PLA General Hospital, Beijing, China; Department of Critical Care Medicine, Peking Union Medical College Hospital, Peking Union Medical College & Chinese Academy of Medical Sciences, Beijing, China.

ABSTRACT

Objectives: The goal of this work was to explore the dynamic concentration profiles of 42 amino acids and the significance of these profiles in relation to sepsis, with the aim of providing guidance for clinical therapies.

Methods: Thirty-five critically ill patients with sepsis were included. These patients were further divided into sepsis (12 cases) and severe sepsis (23 cases) groups or survivor (20 cases) and non-survivor (15 cases) groups. Serum samples from the patients were collected on days 1, 3, 5, 7, 10, and 14 following intensive care unit (ICU) admission, and the serum concentrations of 42 amino acids were measured.

Results: The metabolic spectrum of the amino acids changed dramatically in patients with sepsis. As the disease progressed further or with poor prognosis, the levels of the different amino acids gradually increased, decreased, or fluctuated over time. The concentrations of sulfur-containing amino acids (SAAs), especially taurine, decreased significantly as the severity of sepsis worsened or with poor prognosis of the patient. The serum concentrations of SAAs, especially taurine, exhibited weak negative correlations with the Sequential Organ Failure Assessment (SOFA) (r=-0.319) and Acute Physiology and Chronic Health Evaluation (APACHE) II (r=-0.325) scores. The areas under the receiver operating characteristic curves of cystine, taurine, and SAA levels and the SOFA and APACHE II scores, which denoted disease prognosis, were 0.623, 0.674, 0.678, 0.86, and 0.857, respectively.

Conclusions: Critically ill patients with disorders of amino acid metabolism, especially of SAAs such as cystine and taurine, may provide an indicator of the need for the nutritional support of sepsis in the clinic.

Trial registration: ClinicalTrial.gov identifier NCT01818830.

No MeSH data available.


Related in: MedlinePlus