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Chlorogenic acid improves late diabetes through adiponectin receptor signaling pathways in db/db mice.

Jin S, Chang C, Zhang L, Liu Y, Huang X, Chen Z - PLoS ONE (2015)

Bottom Line: At the end of the intervention, we assessed body fat and the parameters of glucose and lipid metabolism in the plasma, liver and skeletal muscle tissues as well as the levels of aldose reductase (AR) and transforming growth factor-β1 (TGF-β1) in the kidneys and measured adiponectin receptors and the protein expression of their signaling molecules in liver and muscle tissues.After 12 weeks of intervention, compared with the db/db-CON group, the percentage of body fat, fasting plasma glucose (FPG) and glycosylated hemoglobin (HbA1c) in the db/db-CGA group were all significantly decreased; TGF-β1 protein expression and AR activity in the kidney were both decreased; and the adiponectin level in visceral adipose was increased.The protein expression of adiponectin receptors (ADPNRs), the phosphorylation of AMP-activated protein kinase (AMPK) in the liver and muscle, and the mRNA and protein levels of peroxisome proliferator-activated receptor alpha (PPAR-α) in the liver were all significantly greater.

View Article: PubMed Central - PubMed

Affiliation: Institute of Sports Medicine, Peking University Third Hospital, Beijing, China.

ABSTRACT
The aim of this study was to examine the effects of chlorogenic acid (CGA) on glucose and lipid metabolism in late diabetic db/db mice, as well as on adiponectin receptors and their signaling molecules, to provide evidence for CGA in the prevention of type 2 diabetes. We randomly divided 16 female db/db mice into db/db-CGA and db/db-control (CON) groups equally; db/m mice were used as control mice. The mice in both the db/db-CGA and db/m-CGA groups were administered 80 mg/kg/d CGA by lavage for 12 weeks, whereas the mice in both CON groups were given equal volumes of phosphate-buffered saline (PBS) by lavage. At the end of the intervention, we assessed body fat and the parameters of glucose and lipid metabolism in the plasma, liver and skeletal muscle tissues as well as the levels of aldose reductase (AR) and transforming growth factor-β1 (TGF-β1) in the kidneys and measured adiponectin receptors and the protein expression of their signaling molecules in liver and muscle tissues. After 12 weeks of intervention, compared with the db/db-CON group, the percentage of body fat, fasting plasma glucose (FPG) and glycosylated hemoglobin (HbA1c) in the db/db-CGA group were all significantly decreased; TGF-β1 protein expression and AR activity in the kidney were both decreased; and the adiponectin level in visceral adipose was increased. The protein expression of adiponectin receptors (ADPNRs), the phosphorylation of AMP-activated protein kinase (AMPK) in the liver and muscle, and the mRNA and protein levels of peroxisome proliferator-activated receptor alpha (PPAR-α) in the liver were all significantly greater. CGA could lower the levels of fasting plasma glucose and HbA1c during late diabetes and improve kidney fibrosis to some extent through the modulation of adiponectin receptor signaling pathways in db/db mice.

No MeSH data available.


Related in: MedlinePlus

OGTT Test on db/db and db/m Mice after 8 Weeks of Intervention with CGA.An oral glucose tolerance test was performed on db/db and db/m (n = 8 each) mice. 2.5 g/kg glucose was loaded at 0 minutes. Blood samples were collected at 0, 15, 30, 60 and 120 min for glucose measurements. The values are expressed as the mean±SD. *P <0.05.
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pone.0120842.g001: OGTT Test on db/db and db/m Mice after 8 Weeks of Intervention with CGA.An oral glucose tolerance test was performed on db/db and db/m (n = 8 each) mice. 2.5 g/kg glucose was loaded at 0 minutes. Blood samples were collected at 0, 15, 30, 60 and 120 min for glucose measurements. The values are expressed as the mean±SD. *P <0.05.

Mentions: At the end of the experiment, the OGTT results showed that the 15-min peak blood glucose level in the CGA group was decreased by 18.2% (P <0.05) compared with the control group of db/db mice. There were no differences at 30 min, 60 min or 120 min between the two groups (P> 0.05). The glucose AUC in the CGA group showed a non-significant decrease of 10.5% (P> 0.05). The level of plasma glucose was not significantly different in the db/m mice at all time-points (Fig. 1).


Chlorogenic acid improves late diabetes through adiponectin receptor signaling pathways in db/db mice.

Jin S, Chang C, Zhang L, Liu Y, Huang X, Chen Z - PLoS ONE (2015)

OGTT Test on db/db and db/m Mice after 8 Weeks of Intervention with CGA.An oral glucose tolerance test was performed on db/db and db/m (n = 8 each) mice. 2.5 g/kg glucose was loaded at 0 minutes. Blood samples were collected at 0, 15, 30, 60 and 120 min for glucose measurements. The values are expressed as the mean±SD. *P <0.05.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4388831&req=5

pone.0120842.g001: OGTT Test on db/db and db/m Mice after 8 Weeks of Intervention with CGA.An oral glucose tolerance test was performed on db/db and db/m (n = 8 each) mice. 2.5 g/kg glucose was loaded at 0 minutes. Blood samples were collected at 0, 15, 30, 60 and 120 min for glucose measurements. The values are expressed as the mean±SD. *P <0.05.
Mentions: At the end of the experiment, the OGTT results showed that the 15-min peak blood glucose level in the CGA group was decreased by 18.2% (P <0.05) compared with the control group of db/db mice. There were no differences at 30 min, 60 min or 120 min between the two groups (P> 0.05). The glucose AUC in the CGA group showed a non-significant decrease of 10.5% (P> 0.05). The level of plasma glucose was not significantly different in the db/m mice at all time-points (Fig. 1).

Bottom Line: At the end of the intervention, we assessed body fat and the parameters of glucose and lipid metabolism in the plasma, liver and skeletal muscle tissues as well as the levels of aldose reductase (AR) and transforming growth factor-β1 (TGF-β1) in the kidneys and measured adiponectin receptors and the protein expression of their signaling molecules in liver and muscle tissues.After 12 weeks of intervention, compared with the db/db-CON group, the percentage of body fat, fasting plasma glucose (FPG) and glycosylated hemoglobin (HbA1c) in the db/db-CGA group were all significantly decreased; TGF-β1 protein expression and AR activity in the kidney were both decreased; and the adiponectin level in visceral adipose was increased.The protein expression of adiponectin receptors (ADPNRs), the phosphorylation of AMP-activated protein kinase (AMPK) in the liver and muscle, and the mRNA and protein levels of peroxisome proliferator-activated receptor alpha (PPAR-α) in the liver were all significantly greater.

View Article: PubMed Central - PubMed

Affiliation: Institute of Sports Medicine, Peking University Third Hospital, Beijing, China.

ABSTRACT
The aim of this study was to examine the effects of chlorogenic acid (CGA) on glucose and lipid metabolism in late diabetic db/db mice, as well as on adiponectin receptors and their signaling molecules, to provide evidence for CGA in the prevention of type 2 diabetes. We randomly divided 16 female db/db mice into db/db-CGA and db/db-control (CON) groups equally; db/m mice were used as control mice. The mice in both the db/db-CGA and db/m-CGA groups were administered 80 mg/kg/d CGA by lavage for 12 weeks, whereas the mice in both CON groups were given equal volumes of phosphate-buffered saline (PBS) by lavage. At the end of the intervention, we assessed body fat and the parameters of glucose and lipid metabolism in the plasma, liver and skeletal muscle tissues as well as the levels of aldose reductase (AR) and transforming growth factor-β1 (TGF-β1) in the kidneys and measured adiponectin receptors and the protein expression of their signaling molecules in liver and muscle tissues. After 12 weeks of intervention, compared with the db/db-CON group, the percentage of body fat, fasting plasma glucose (FPG) and glycosylated hemoglobin (HbA1c) in the db/db-CGA group were all significantly decreased; TGF-β1 protein expression and AR activity in the kidney were both decreased; and the adiponectin level in visceral adipose was increased. The protein expression of adiponectin receptors (ADPNRs), the phosphorylation of AMP-activated protein kinase (AMPK) in the liver and muscle, and the mRNA and protein levels of peroxisome proliferator-activated receptor alpha (PPAR-α) in the liver were all significantly greater. CGA could lower the levels of fasting plasma glucose and HbA1c during late diabetes and improve kidney fibrosis to some extent through the modulation of adiponectin receptor signaling pathways in db/db mice.

No MeSH data available.


Related in: MedlinePlus