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Significant improvement of biocompatibility of polypropylene mesh for incisional hernia repair by using poly-ε-caprolactone nanofibers functionalized with thrombocyte-rich solution.

Plencner M, Prosecká E, Rampichová M, East B, Buzgo M, Vysloužilová L, Hoch J, Amler E - Int J Nanomedicine (2015)

Bottom Line: Nonetheless, the ideal mesh does not exist yet; it still needs to be developed.Compared with polypropylene mesh alone, this composite scaffold provided better adhesion, growth, metabolic activity, proliferation, and viability of mouse fibroblasts in all tests and was even better than a polypropylene mesh functionalized with PCL nanofibers.The gradual release of growth factors from biocompatible nanofiber-modified scaffolds seems to be a promising approach in tissue engineering and regenerative medicine.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biophysics, 2nd Faculty of Medicine, Charles University in Prague, Prague, Czech Republic ; Laboratory of Tissue Engineering, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic.

ABSTRACT
Incisional hernia is the most common postoperative complication, affecting up to 20% of patients after abdominal surgery. Insertion of a synthetic surgical mesh has become the standard of care in ventral hernia repair. However, the implementation of a mesh does not reduce the risk of recurrence and the onset of hernia recurrence is only delayed by 2-3 years. Nowadays, more than 100 surgical meshes are available on the market, with polypropylene the most widely used for ventral hernia repair. Nonetheless, the ideal mesh does not exist yet; it still needs to be developed. Polycaprolactone nanofibers appear to be a suitable material for different kinds of cells, including fibroblasts, chondrocytes, and mesenchymal stem cells. The aim of the study reported here was to develop a functionalized scaffold for ventral hernia regeneration. We prepared a novel composite scaffold based on a polypropylene surgical mesh functionalized with poly-ε-caprolactone (PCL) nanofibers and adhered thrombocytes as a natural source of growth factors. In extensive in vitro tests, we proved the biocompatibility of PCL nanofibers with adhered thrombocytes deposited on a polypropylene mesh. Compared with polypropylene mesh alone, this composite scaffold provided better adhesion, growth, metabolic activity, proliferation, and viability of mouse fibroblasts in all tests and was even better than a polypropylene mesh functionalized with PCL nanofibers. The gradual release of growth factors from biocompatible nanofiber-modified scaffolds seems to be a promising approach in tissue engineering and regenerative medicine.

No MeSH data available.


Related in: MedlinePlus

Proliferation of 3T3 fibroblasts cultivated on the surface of (1) polypropylene (PP) mesh, (2) PP mesh treated with thrombocyte-rich solution (TRS), (3) PP mesh functionalized with poly-ε-caprolactone (PCL) nanofibers, and (4) PP mesh functionalized with PCL nanofibers treated with TRS. A 5-bromo-2′-deoxyuridine (BrdU) colorimetric immunoassay revealed significantly greater proliferation of 3T3 fibroblasts on scaffolds functionalized with PCL nanofibers (PP + PCL and PP + PCL + TRS) on all days of evaluation than on scaffolds without functionalization (PP and PP + TRS). In addition, the proliferation of 3T3 fibroblasts on Day 14 was significantly higher (P<0.001) on the PP mesh functionalized with PCL nanofibers treated with TRS than on all other scaffolds.Notes: The level of statistical significance for the assays is designated above the mean values (P<0.05 indicated by a number, P<0.001 indicated by a number and *). Day 1: 3>1*, 2*; 4>1*, 2*. Day 3: 3>1*, 2; 4>1*, 2*. Day 7: 3>1*, 2*; 4>1*, 2. Day 10: 3>1*, 2*; 4>1*, 2*, 3. Day 14: 2>1*; 3>1*, 2; 4>1*, 2*, 3*.Abbreviation: AU, absorbance units.
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f7-ijn-10-2635: Proliferation of 3T3 fibroblasts cultivated on the surface of (1) polypropylene (PP) mesh, (2) PP mesh treated with thrombocyte-rich solution (TRS), (3) PP mesh functionalized with poly-ε-caprolactone (PCL) nanofibers, and (4) PP mesh functionalized with PCL nanofibers treated with TRS. A 5-bromo-2′-deoxyuridine (BrdU) colorimetric immunoassay revealed significantly greater proliferation of 3T3 fibroblasts on scaffolds functionalized with PCL nanofibers (PP + PCL and PP + PCL + TRS) on all days of evaluation than on scaffolds without functionalization (PP and PP + TRS). In addition, the proliferation of 3T3 fibroblasts on Day 14 was significantly higher (P<0.001) on the PP mesh functionalized with PCL nanofibers treated with TRS than on all other scaffolds.Notes: The level of statistical significance for the assays is designated above the mean values (P<0.05 indicated by a number, P<0.001 indicated by a number and *). Day 1: 3>1*, 2*; 4>1*, 2*. Day 3: 3>1*, 2; 4>1*, 2*. Day 7: 3>1*, 2*; 4>1*, 2. Day 10: 3>1*, 2*; 4>1*, 2*, 3. Day 14: 2>1*; 3>1*, 2; 4>1*, 2*, 3*.Abbreviation: AU, absorbance units.

Mentions: Cell metabolic activity can result not only in a larger number of cells, as was confirmed by our study of DNA content, but also an increase in cell proliferation. Cell proliferation was therefore evaluated using a BrdU colorimetric immunoassay. This assay is based on incorporating bromodeoxyuridine only in the active process of DNA synthesis in healthy cells. BrdU colorimetric immunoassay was performed on Days 1, 3, 7, 10, and 14 (Figure 7). The BrdU assay revealed significantly higher proliferation of 3T3 fibroblasts on all evaluation days on scaffolds functionalized with PCL nanofibers and on scaffolds with PCL nanofibers treated with TRS than on scaffolds without functionalization – namely, the PP mesh alone and the PP mesh treated with TRS. Moreover, the proliferation of 3T3 fibroblasts on Day 14 was significantly higher (P<0.001) on the PP mesh functionalized with PCL nanofibers and treated with TRS than on all other scaffolds. Thus, PP mesh functionalization also significantly improved 3T3 fibroblast proliferation.


Significant improvement of biocompatibility of polypropylene mesh for incisional hernia repair by using poly-ε-caprolactone nanofibers functionalized with thrombocyte-rich solution.

Plencner M, Prosecká E, Rampichová M, East B, Buzgo M, Vysloužilová L, Hoch J, Amler E - Int J Nanomedicine (2015)

Proliferation of 3T3 fibroblasts cultivated on the surface of (1) polypropylene (PP) mesh, (2) PP mesh treated with thrombocyte-rich solution (TRS), (3) PP mesh functionalized with poly-ε-caprolactone (PCL) nanofibers, and (4) PP mesh functionalized with PCL nanofibers treated with TRS. A 5-bromo-2′-deoxyuridine (BrdU) colorimetric immunoassay revealed significantly greater proliferation of 3T3 fibroblasts on scaffolds functionalized with PCL nanofibers (PP + PCL and PP + PCL + TRS) on all days of evaluation than on scaffolds without functionalization (PP and PP + TRS). In addition, the proliferation of 3T3 fibroblasts on Day 14 was significantly higher (P<0.001) on the PP mesh functionalized with PCL nanofibers treated with TRS than on all other scaffolds.Notes: The level of statistical significance for the assays is designated above the mean values (P<0.05 indicated by a number, P<0.001 indicated by a number and *). Day 1: 3>1*, 2*; 4>1*, 2*. Day 3: 3>1*, 2; 4>1*, 2*. Day 7: 3>1*, 2*; 4>1*, 2. Day 10: 3>1*, 2*; 4>1*, 2*, 3. Day 14: 2>1*; 3>1*, 2; 4>1*, 2*, 3*.Abbreviation: AU, absorbance units.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4388102&req=5

f7-ijn-10-2635: Proliferation of 3T3 fibroblasts cultivated on the surface of (1) polypropylene (PP) mesh, (2) PP mesh treated with thrombocyte-rich solution (TRS), (3) PP mesh functionalized with poly-ε-caprolactone (PCL) nanofibers, and (4) PP mesh functionalized with PCL nanofibers treated with TRS. A 5-bromo-2′-deoxyuridine (BrdU) colorimetric immunoassay revealed significantly greater proliferation of 3T3 fibroblasts on scaffolds functionalized with PCL nanofibers (PP + PCL and PP + PCL + TRS) on all days of evaluation than on scaffolds without functionalization (PP and PP + TRS). In addition, the proliferation of 3T3 fibroblasts on Day 14 was significantly higher (P<0.001) on the PP mesh functionalized with PCL nanofibers treated with TRS than on all other scaffolds.Notes: The level of statistical significance for the assays is designated above the mean values (P<0.05 indicated by a number, P<0.001 indicated by a number and *). Day 1: 3>1*, 2*; 4>1*, 2*. Day 3: 3>1*, 2; 4>1*, 2*. Day 7: 3>1*, 2*; 4>1*, 2. Day 10: 3>1*, 2*; 4>1*, 2*, 3. Day 14: 2>1*; 3>1*, 2; 4>1*, 2*, 3*.Abbreviation: AU, absorbance units.
Mentions: Cell metabolic activity can result not only in a larger number of cells, as was confirmed by our study of DNA content, but also an increase in cell proliferation. Cell proliferation was therefore evaluated using a BrdU colorimetric immunoassay. This assay is based on incorporating bromodeoxyuridine only in the active process of DNA synthesis in healthy cells. BrdU colorimetric immunoassay was performed on Days 1, 3, 7, 10, and 14 (Figure 7). The BrdU assay revealed significantly higher proliferation of 3T3 fibroblasts on all evaluation days on scaffolds functionalized with PCL nanofibers and on scaffolds with PCL nanofibers treated with TRS than on scaffolds without functionalization – namely, the PP mesh alone and the PP mesh treated with TRS. Moreover, the proliferation of 3T3 fibroblasts on Day 14 was significantly higher (P<0.001) on the PP mesh functionalized with PCL nanofibers and treated with TRS than on all other scaffolds. Thus, PP mesh functionalization also significantly improved 3T3 fibroblast proliferation.

Bottom Line: Nonetheless, the ideal mesh does not exist yet; it still needs to be developed.Compared with polypropylene mesh alone, this composite scaffold provided better adhesion, growth, metabolic activity, proliferation, and viability of mouse fibroblasts in all tests and was even better than a polypropylene mesh functionalized with PCL nanofibers.The gradual release of growth factors from biocompatible nanofiber-modified scaffolds seems to be a promising approach in tissue engineering and regenerative medicine.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biophysics, 2nd Faculty of Medicine, Charles University in Prague, Prague, Czech Republic ; Laboratory of Tissue Engineering, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic.

ABSTRACT
Incisional hernia is the most common postoperative complication, affecting up to 20% of patients after abdominal surgery. Insertion of a synthetic surgical mesh has become the standard of care in ventral hernia repair. However, the implementation of a mesh does not reduce the risk of recurrence and the onset of hernia recurrence is only delayed by 2-3 years. Nowadays, more than 100 surgical meshes are available on the market, with polypropylene the most widely used for ventral hernia repair. Nonetheless, the ideal mesh does not exist yet; it still needs to be developed. Polycaprolactone nanofibers appear to be a suitable material for different kinds of cells, including fibroblasts, chondrocytes, and mesenchymal stem cells. The aim of the study reported here was to develop a functionalized scaffold for ventral hernia regeneration. We prepared a novel composite scaffold based on a polypropylene surgical mesh functionalized with poly-ε-caprolactone (PCL) nanofibers and adhered thrombocytes as a natural source of growth factors. In extensive in vitro tests, we proved the biocompatibility of PCL nanofibers with adhered thrombocytes deposited on a polypropylene mesh. Compared with polypropylene mesh alone, this composite scaffold provided better adhesion, growth, metabolic activity, proliferation, and viability of mouse fibroblasts in all tests and was even better than a polypropylene mesh functionalized with PCL nanofibers. The gradual release of growth factors from biocompatible nanofiber-modified scaffolds seems to be a promising approach in tissue engineering and regenerative medicine.

No MeSH data available.


Related in: MedlinePlus