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Significant improvement of biocompatibility of polypropylene mesh for incisional hernia repair by using poly-ε-caprolactone nanofibers functionalized with thrombocyte-rich solution.

Plencner M, Prosecká E, Rampichová M, East B, Buzgo M, Vysloužilová L, Hoch J, Amler E - Int J Nanomedicine (2015)

Bottom Line: Nonetheless, the ideal mesh does not exist yet; it still needs to be developed.Compared with polypropylene mesh alone, this composite scaffold provided better adhesion, growth, metabolic activity, proliferation, and viability of mouse fibroblasts in all tests and was even better than a polypropylene mesh functionalized with PCL nanofibers.The gradual release of growth factors from biocompatible nanofiber-modified scaffolds seems to be a promising approach in tissue engineering and regenerative medicine.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biophysics, 2nd Faculty of Medicine, Charles University in Prague, Prague, Czech Republic ; Laboratory of Tissue Engineering, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic.

ABSTRACT
Incisional hernia is the most common postoperative complication, affecting up to 20% of patients after abdominal surgery. Insertion of a synthetic surgical mesh has become the standard of care in ventral hernia repair. However, the implementation of a mesh does not reduce the risk of recurrence and the onset of hernia recurrence is only delayed by 2-3 years. Nowadays, more than 100 surgical meshes are available on the market, with polypropylene the most widely used for ventral hernia repair. Nonetheless, the ideal mesh does not exist yet; it still needs to be developed. Polycaprolactone nanofibers appear to be a suitable material for different kinds of cells, including fibroblasts, chondrocytes, and mesenchymal stem cells. The aim of the study reported here was to develop a functionalized scaffold for ventral hernia regeneration. We prepared a novel composite scaffold based on a polypropylene surgical mesh functionalized with poly-ε-caprolactone (PCL) nanofibers and adhered thrombocytes as a natural source of growth factors. In extensive in vitro tests, we proved the biocompatibility of PCL nanofibers with adhered thrombocytes deposited on a polypropylene mesh. Compared with polypropylene mesh alone, this composite scaffold provided better adhesion, growth, metabolic activity, proliferation, and viability of mouse fibroblasts in all tests and was even better than a polypropylene mesh functionalized with PCL nanofibers. The gradual release of growth factors from biocompatible nanofiber-modified scaffolds seems to be a promising approach in tissue engineering and regenerative medicine.

No MeSH data available.


Related in: MedlinePlus

Metabolic activity of 3T3 fibroblasts cultivated on the surface of (1) polypropylene (PP) mesh, (2) PP mesh enriched with adhered thrombocytes, (3) PP mesh functionalized with poly-ε-caprolactone (PCL) nanofibers, and (4) PP mesh functionalized with PCL nanofibers enriched with adhered thrombocytes. 3-[4,5-dimethylthiazol- 2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay revealed significantly higher metabolic activity of 3T3 fibroblasts on scaffolds functionalized with PCL nanofibers (PP + PCL and PP + PCL + thrombocyte-rich solution [TRS]) on Day 14 than on scaffolds without functionalization (PP). Moreover, the metabolic activity of 3T3 fibroblasts on Days 7, 10, and 14 was significantly higher on the PP mesh functionalized with PCL nanofibers treated with TRS than on all other scaffolds.Notes: The level of statistical significance for the assays is designated above the mean values (P<0.05 indicated by a number; P<0.001 indicated by a number and *). Day 1: without significance. Day 3: 3>1, 2; 4>1*, 2*. Day 7: 3>1; 4>1*, 2*, 3. Day 10: 2>1; 3>1*; 4>1*, 2, 3. Day 14: 2>1*; 3>1*, 2*; 4>1*, 2*, 3.
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f4-ijn-10-2635: Metabolic activity of 3T3 fibroblasts cultivated on the surface of (1) polypropylene (PP) mesh, (2) PP mesh enriched with adhered thrombocytes, (3) PP mesh functionalized with poly-ε-caprolactone (PCL) nanofibers, and (4) PP mesh functionalized with PCL nanofibers enriched with adhered thrombocytes. 3-[4,5-dimethylthiazol- 2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay revealed significantly higher metabolic activity of 3T3 fibroblasts on scaffolds functionalized with PCL nanofibers (PP + PCL and PP + PCL + thrombocyte-rich solution [TRS]) on Day 14 than on scaffolds without functionalization (PP). Moreover, the metabolic activity of 3T3 fibroblasts on Days 7, 10, and 14 was significantly higher on the PP mesh functionalized with PCL nanofibers treated with TRS than on all other scaffolds.Notes: The level of statistical significance for the assays is designated above the mean values (P<0.05 indicated by a number; P<0.001 indicated by a number and *). Day 1: without significance. Day 3: 3>1, 2; 4>1*, 2*. Day 7: 3>1; 4>1*, 2*, 3. Day 10: 2>1; 3>1*; 4>1*, 2, 3. Day 14: 2>1*; 3>1*, 2*; 4>1*, 2*, 3.

Mentions: Metabolic activity of the 3T3 fibroblasts was determined by MTT assay. The MTT assay revealed significantly higher metabolic activity of the cells after the 10th day of cultivation on all of the composite scaffolds than on the PP mesh alone (Figure 4). Moreover, significantly higher (P<0.001) cell metabolic activity was observed on all of the functionalized composite scaffolds on Day 14. Significantly higher metabolic activity was also observed on the PP meshes functionalized with PCL nanofibers and the PP meshes functionalized with PCL nanofibers treated with TRS after the 7th day of cultivation. Additionally, a combination of two improvements to PP mesh – namely, functionalization with PCL nanofibers and treatment with TRS – led to significantly higher (P<0.001) cell metabolic activity on Day 14 than that on all other scaffolds that were used.


Significant improvement of biocompatibility of polypropylene mesh for incisional hernia repair by using poly-ε-caprolactone nanofibers functionalized with thrombocyte-rich solution.

Plencner M, Prosecká E, Rampichová M, East B, Buzgo M, Vysloužilová L, Hoch J, Amler E - Int J Nanomedicine (2015)

Metabolic activity of 3T3 fibroblasts cultivated on the surface of (1) polypropylene (PP) mesh, (2) PP mesh enriched with adhered thrombocytes, (3) PP mesh functionalized with poly-ε-caprolactone (PCL) nanofibers, and (4) PP mesh functionalized with PCL nanofibers enriched with adhered thrombocytes. 3-[4,5-dimethylthiazol- 2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay revealed significantly higher metabolic activity of 3T3 fibroblasts on scaffolds functionalized with PCL nanofibers (PP + PCL and PP + PCL + thrombocyte-rich solution [TRS]) on Day 14 than on scaffolds without functionalization (PP). Moreover, the metabolic activity of 3T3 fibroblasts on Days 7, 10, and 14 was significantly higher on the PP mesh functionalized with PCL nanofibers treated with TRS than on all other scaffolds.Notes: The level of statistical significance for the assays is designated above the mean values (P<0.05 indicated by a number; P<0.001 indicated by a number and *). Day 1: without significance. Day 3: 3>1, 2; 4>1*, 2*. Day 7: 3>1; 4>1*, 2*, 3. Day 10: 2>1; 3>1*; 4>1*, 2, 3. Day 14: 2>1*; 3>1*, 2*; 4>1*, 2*, 3.
© Copyright Policy
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4388102&req=5

f4-ijn-10-2635: Metabolic activity of 3T3 fibroblasts cultivated on the surface of (1) polypropylene (PP) mesh, (2) PP mesh enriched with adhered thrombocytes, (3) PP mesh functionalized with poly-ε-caprolactone (PCL) nanofibers, and (4) PP mesh functionalized with PCL nanofibers enriched with adhered thrombocytes. 3-[4,5-dimethylthiazol- 2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay revealed significantly higher metabolic activity of 3T3 fibroblasts on scaffolds functionalized with PCL nanofibers (PP + PCL and PP + PCL + thrombocyte-rich solution [TRS]) on Day 14 than on scaffolds without functionalization (PP). Moreover, the metabolic activity of 3T3 fibroblasts on Days 7, 10, and 14 was significantly higher on the PP mesh functionalized with PCL nanofibers treated with TRS than on all other scaffolds.Notes: The level of statistical significance for the assays is designated above the mean values (P<0.05 indicated by a number; P<0.001 indicated by a number and *). Day 1: without significance. Day 3: 3>1, 2; 4>1*, 2*. Day 7: 3>1; 4>1*, 2*, 3. Day 10: 2>1; 3>1*; 4>1*, 2, 3. Day 14: 2>1*; 3>1*, 2*; 4>1*, 2*, 3.
Mentions: Metabolic activity of the 3T3 fibroblasts was determined by MTT assay. The MTT assay revealed significantly higher metabolic activity of the cells after the 10th day of cultivation on all of the composite scaffolds than on the PP mesh alone (Figure 4). Moreover, significantly higher (P<0.001) cell metabolic activity was observed on all of the functionalized composite scaffolds on Day 14. Significantly higher metabolic activity was also observed on the PP meshes functionalized with PCL nanofibers and the PP meshes functionalized with PCL nanofibers treated with TRS after the 7th day of cultivation. Additionally, a combination of two improvements to PP mesh – namely, functionalization with PCL nanofibers and treatment with TRS – led to significantly higher (P<0.001) cell metabolic activity on Day 14 than that on all other scaffolds that were used.

Bottom Line: Nonetheless, the ideal mesh does not exist yet; it still needs to be developed.Compared with polypropylene mesh alone, this composite scaffold provided better adhesion, growth, metabolic activity, proliferation, and viability of mouse fibroblasts in all tests and was even better than a polypropylene mesh functionalized with PCL nanofibers.The gradual release of growth factors from biocompatible nanofiber-modified scaffolds seems to be a promising approach in tissue engineering and regenerative medicine.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biophysics, 2nd Faculty of Medicine, Charles University in Prague, Prague, Czech Republic ; Laboratory of Tissue Engineering, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic.

ABSTRACT
Incisional hernia is the most common postoperative complication, affecting up to 20% of patients after abdominal surgery. Insertion of a synthetic surgical mesh has become the standard of care in ventral hernia repair. However, the implementation of a mesh does not reduce the risk of recurrence and the onset of hernia recurrence is only delayed by 2-3 years. Nowadays, more than 100 surgical meshes are available on the market, with polypropylene the most widely used for ventral hernia repair. Nonetheless, the ideal mesh does not exist yet; it still needs to be developed. Polycaprolactone nanofibers appear to be a suitable material for different kinds of cells, including fibroblasts, chondrocytes, and mesenchymal stem cells. The aim of the study reported here was to develop a functionalized scaffold for ventral hernia regeneration. We prepared a novel composite scaffold based on a polypropylene surgical mesh functionalized with poly-ε-caprolactone (PCL) nanofibers and adhered thrombocytes as a natural source of growth factors. In extensive in vitro tests, we proved the biocompatibility of PCL nanofibers with adhered thrombocytes deposited on a polypropylene mesh. Compared with polypropylene mesh alone, this composite scaffold provided better adhesion, growth, metabolic activity, proliferation, and viability of mouse fibroblasts in all tests and was even better than a polypropylene mesh functionalized with PCL nanofibers. The gradual release of growth factors from biocompatible nanofiber-modified scaffolds seems to be a promising approach in tissue engineering and regenerative medicine.

No MeSH data available.


Related in: MedlinePlus