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Significant improvement of biocompatibility of polypropylene mesh for incisional hernia repair by using poly-ε-caprolactone nanofibers functionalized with thrombocyte-rich solution.

Plencner M, Prosecká E, Rampichová M, East B, Buzgo M, Vysloužilová L, Hoch J, Amler E - Int J Nanomedicine (2015)

Bottom Line: Nonetheless, the ideal mesh does not exist yet; it still needs to be developed.Compared with polypropylene mesh alone, this composite scaffold provided better adhesion, growth, metabolic activity, proliferation, and viability of mouse fibroblasts in all tests and was even better than a polypropylene mesh functionalized with PCL nanofibers.The gradual release of growth factors from biocompatible nanofiber-modified scaffolds seems to be a promising approach in tissue engineering and regenerative medicine.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biophysics, 2nd Faculty of Medicine, Charles University in Prague, Prague, Czech Republic ; Laboratory of Tissue Engineering, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic.

ABSTRACT
Incisional hernia is the most common postoperative complication, affecting up to 20% of patients after abdominal surgery. Insertion of a synthetic surgical mesh has become the standard of care in ventral hernia repair. However, the implementation of a mesh does not reduce the risk of recurrence and the onset of hernia recurrence is only delayed by 2-3 years. Nowadays, more than 100 surgical meshes are available on the market, with polypropylene the most widely used for ventral hernia repair. Nonetheless, the ideal mesh does not exist yet; it still needs to be developed. Polycaprolactone nanofibers appear to be a suitable material for different kinds of cells, including fibroblasts, chondrocytes, and mesenchymal stem cells. The aim of the study reported here was to develop a functionalized scaffold for ventral hernia regeneration. We prepared a novel composite scaffold based on a polypropylene surgical mesh functionalized with poly-ε-caprolactone (PCL) nanofibers and adhered thrombocytes as a natural source of growth factors. In extensive in vitro tests, we proved the biocompatibility of PCL nanofibers with adhered thrombocytes deposited on a polypropylene mesh. Compared with polypropylene mesh alone, this composite scaffold provided better adhesion, growth, metabolic activity, proliferation, and viability of mouse fibroblasts in all tests and was even better than a polypropylene mesh functionalized with PCL nanofibers. The gradual release of growth factors from biocompatible nanofiber-modified scaffolds seems to be a promising approach in tissue engineering and regenerative medicine.

No MeSH data available.


Related in: MedlinePlus

Cell adhesion evaluated on the 1st day after seeding. (1) Average surface area of spread 3T3 fibroblasts cultivated on the surface of a polypropylene (PP) mesh, (2) a PP mesh treated with thrombocyte-rich solution (TRS), (3) a PP mesh functionalized with poly-ε-caprolactone (PCL) nanofibers, and (4) a PP mesh functionalized with PCL nanofibers treated with TRS. Cell adhesion assay revealed a significantly larger surface area of spread 3T3 fibroblasts on scaffolds functionalized with PCL nanofibers (PP + PCL and PP + PCL + TRS) than on scaffolds without functionalization (PP and PP + TRS). Moreover, the average surface area of 3T3 fibroblasts was significantly higher (level of significance at a value of P<0.001) on the PP mesh functionalized with PCL nanofibers treated with TRS than on all other scaffolds.Notes: The level of statistical significance for the assays is designated above the mean values (P<0.05 indicated by number; P<0.001 indicated by number and *). Day 1: 2>1*; 3>1*, 2; 4>1*, 2*, 3*.
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f3-ijn-10-2635: Cell adhesion evaluated on the 1st day after seeding. (1) Average surface area of spread 3T3 fibroblasts cultivated on the surface of a polypropylene (PP) mesh, (2) a PP mesh treated with thrombocyte-rich solution (TRS), (3) a PP mesh functionalized with poly-ε-caprolactone (PCL) nanofibers, and (4) a PP mesh functionalized with PCL nanofibers treated with TRS. Cell adhesion assay revealed a significantly larger surface area of spread 3T3 fibroblasts on scaffolds functionalized with PCL nanofibers (PP + PCL and PP + PCL + TRS) than on scaffolds without functionalization (PP and PP + TRS). Moreover, the average surface area of 3T3 fibroblasts was significantly higher (level of significance at a value of P<0.001) on the PP mesh functionalized with PCL nanofibers treated with TRS than on all other scaffolds.Notes: The level of statistical significance for the assays is designated above the mean values (P<0.05 indicated by number; P<0.001 indicated by number and *). Day 1: 2>1*; 3>1*, 2; 4>1*, 2*, 3*.

Mentions: First, cell adhesion was evaluated 24 hours after seeding. The 3T3 fibroblasts were first stained using DiOC6 and propidium iodide, then visualized using confocal microscopy before areas of the spread cell surface were measured. A significantly larger surface area of the cells was observed on the PP mesh functionalized with PCL nanofibers than on the PP mesh alone (Figure 3). In addition, the largest spreading area was for the cells cultivated on the PP mesh functionalized with PCL nanofibers and TRS. Simultaneously, enrichment of the PP mesh with TRS improved cell adhesion, and the cell spreading area of PP + TRS was significantly larger than for PP (P<0.001). Clearly, functionalization of the PP meshes with PCL nanofibers alone, and also treatment of PP meshes with TRS, significantly improved 3T3 adhesion. These two actions seem to be independent, and can therefore result in an additive effect.


Significant improvement of biocompatibility of polypropylene mesh for incisional hernia repair by using poly-ε-caprolactone nanofibers functionalized with thrombocyte-rich solution.

Plencner M, Prosecká E, Rampichová M, East B, Buzgo M, Vysloužilová L, Hoch J, Amler E - Int J Nanomedicine (2015)

Cell adhesion evaluated on the 1st day after seeding. (1) Average surface area of spread 3T3 fibroblasts cultivated on the surface of a polypropylene (PP) mesh, (2) a PP mesh treated with thrombocyte-rich solution (TRS), (3) a PP mesh functionalized with poly-ε-caprolactone (PCL) nanofibers, and (4) a PP mesh functionalized with PCL nanofibers treated with TRS. Cell adhesion assay revealed a significantly larger surface area of spread 3T3 fibroblasts on scaffolds functionalized with PCL nanofibers (PP + PCL and PP + PCL + TRS) than on scaffolds without functionalization (PP and PP + TRS). Moreover, the average surface area of 3T3 fibroblasts was significantly higher (level of significance at a value of P<0.001) on the PP mesh functionalized with PCL nanofibers treated with TRS than on all other scaffolds.Notes: The level of statistical significance for the assays is designated above the mean values (P<0.05 indicated by number; P<0.001 indicated by number and *). Day 1: 2>1*; 3>1*, 2; 4>1*, 2*, 3*.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4388102&req=5

f3-ijn-10-2635: Cell adhesion evaluated on the 1st day after seeding. (1) Average surface area of spread 3T3 fibroblasts cultivated on the surface of a polypropylene (PP) mesh, (2) a PP mesh treated with thrombocyte-rich solution (TRS), (3) a PP mesh functionalized with poly-ε-caprolactone (PCL) nanofibers, and (4) a PP mesh functionalized with PCL nanofibers treated with TRS. Cell adhesion assay revealed a significantly larger surface area of spread 3T3 fibroblasts on scaffolds functionalized with PCL nanofibers (PP + PCL and PP + PCL + TRS) than on scaffolds without functionalization (PP and PP + TRS). Moreover, the average surface area of 3T3 fibroblasts was significantly higher (level of significance at a value of P<0.001) on the PP mesh functionalized with PCL nanofibers treated with TRS than on all other scaffolds.Notes: The level of statistical significance for the assays is designated above the mean values (P<0.05 indicated by number; P<0.001 indicated by number and *). Day 1: 2>1*; 3>1*, 2; 4>1*, 2*, 3*.
Mentions: First, cell adhesion was evaluated 24 hours after seeding. The 3T3 fibroblasts were first stained using DiOC6 and propidium iodide, then visualized using confocal microscopy before areas of the spread cell surface were measured. A significantly larger surface area of the cells was observed on the PP mesh functionalized with PCL nanofibers than on the PP mesh alone (Figure 3). In addition, the largest spreading area was for the cells cultivated on the PP mesh functionalized with PCL nanofibers and TRS. Simultaneously, enrichment of the PP mesh with TRS improved cell adhesion, and the cell spreading area of PP + TRS was significantly larger than for PP (P<0.001). Clearly, functionalization of the PP meshes with PCL nanofibers alone, and also treatment of PP meshes with TRS, significantly improved 3T3 adhesion. These two actions seem to be independent, and can therefore result in an additive effect.

Bottom Line: Nonetheless, the ideal mesh does not exist yet; it still needs to be developed.Compared with polypropylene mesh alone, this composite scaffold provided better adhesion, growth, metabolic activity, proliferation, and viability of mouse fibroblasts in all tests and was even better than a polypropylene mesh functionalized with PCL nanofibers.The gradual release of growth factors from biocompatible nanofiber-modified scaffolds seems to be a promising approach in tissue engineering and regenerative medicine.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biophysics, 2nd Faculty of Medicine, Charles University in Prague, Prague, Czech Republic ; Laboratory of Tissue Engineering, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic.

ABSTRACT
Incisional hernia is the most common postoperative complication, affecting up to 20% of patients after abdominal surgery. Insertion of a synthetic surgical mesh has become the standard of care in ventral hernia repair. However, the implementation of a mesh does not reduce the risk of recurrence and the onset of hernia recurrence is only delayed by 2-3 years. Nowadays, more than 100 surgical meshes are available on the market, with polypropylene the most widely used for ventral hernia repair. Nonetheless, the ideal mesh does not exist yet; it still needs to be developed. Polycaprolactone nanofibers appear to be a suitable material for different kinds of cells, including fibroblasts, chondrocytes, and mesenchymal stem cells. The aim of the study reported here was to develop a functionalized scaffold for ventral hernia regeneration. We prepared a novel composite scaffold based on a polypropylene surgical mesh functionalized with poly-ε-caprolactone (PCL) nanofibers and adhered thrombocytes as a natural source of growth factors. In extensive in vitro tests, we proved the biocompatibility of PCL nanofibers with adhered thrombocytes deposited on a polypropylene mesh. Compared with polypropylene mesh alone, this composite scaffold provided better adhesion, growth, metabolic activity, proliferation, and viability of mouse fibroblasts in all tests and was even better than a polypropylene mesh functionalized with PCL nanofibers. The gradual release of growth factors from biocompatible nanofiber-modified scaffolds seems to be a promising approach in tissue engineering and regenerative medicine.

No MeSH data available.


Related in: MedlinePlus