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Significant improvement of biocompatibility of polypropylene mesh for incisional hernia repair by using poly-ε-caprolactone nanofibers functionalized with thrombocyte-rich solution.

Plencner M, Prosecká E, Rampichová M, East B, Buzgo M, Vysloužilová L, Hoch J, Amler E - Int J Nanomedicine (2015)

Bottom Line: Nonetheless, the ideal mesh does not exist yet; it still needs to be developed.Compared with polypropylene mesh alone, this composite scaffold provided better adhesion, growth, metabolic activity, proliferation, and viability of mouse fibroblasts in all tests and was even better than a polypropylene mesh functionalized with PCL nanofibers.The gradual release of growth factors from biocompatible nanofiber-modified scaffolds seems to be a promising approach in tissue engineering and regenerative medicine.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biophysics, 2nd Faculty of Medicine, Charles University in Prague, Prague, Czech Republic ; Laboratory of Tissue Engineering, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic.

ABSTRACT
Incisional hernia is the most common postoperative complication, affecting up to 20% of patients after abdominal surgery. Insertion of a synthetic surgical mesh has become the standard of care in ventral hernia repair. However, the implementation of a mesh does not reduce the risk of recurrence and the onset of hernia recurrence is only delayed by 2-3 years. Nowadays, more than 100 surgical meshes are available on the market, with polypropylene the most widely used for ventral hernia repair. Nonetheless, the ideal mesh does not exist yet; it still needs to be developed. Polycaprolactone nanofibers appear to be a suitable material for different kinds of cells, including fibroblasts, chondrocytes, and mesenchymal stem cells. The aim of the study reported here was to develop a functionalized scaffold for ventral hernia regeneration. We prepared a novel composite scaffold based on a polypropylene surgical mesh functionalized with poly-ε-caprolactone (PCL) nanofibers and adhered thrombocytes as a natural source of growth factors. In extensive in vitro tests, we proved the biocompatibility of PCL nanofibers with adhered thrombocytes deposited on a polypropylene mesh. Compared with polypropylene mesh alone, this composite scaffold provided better adhesion, growth, metabolic activity, proliferation, and viability of mouse fibroblasts in all tests and was even better than a polypropylene mesh functionalized with PCL nanofibers. The gradual release of growth factors from biocompatible nanofiber-modified scaffolds seems to be a promising approach in tissue engineering and regenerative medicine.

No MeSH data available.


Related in: MedlinePlus

Scanning electron microscopy of the scaffolds. (A) Poly-ε-caprolactone (PCL) nanofibers, (B) polypropylene (PP) mesh, and (C) PP mesh functionalized with PCL nanofibers.Notes: (A) Magnification ×230, scale bar 50 μm; (B and C) magnification ×18, scale bar 500 μm.
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f2-ijn-10-2635: Scanning electron microscopy of the scaffolds. (A) Poly-ε-caprolactone (PCL) nanofibers, (B) polypropylene (PP) mesh, and (C) PP mesh functionalized with PCL nanofibers.Notes: (A) Magnification ×230, scale bar 50 μm; (B and C) magnification ×18, scale bar 500 μm.

Mentions: The functionalized mesh was prepared by attaching the PP mesh onto the collector before the electrospinning process. PCL nanofibers were deposited on the surface of the PP mesh. The PP mesh with nanofibers was exposed in an aqueous environment for 2 weeks without any visual effect on the functionalized mesh. Three types of functionalized scaffolds were examined using SEM in secondary electron mode. Samples of the PP mesh (Figure 2B) were functionalized with PCL nanofibers (Figure 2A) to create a composite scaffold (Figure 2C). SEM revealed randomly oriented nanofibers and their deposition onto the PP mesh. Stereological analyses divided the nanofibers into two fractions of PCL fibers. The first fraction contained fibers with an average diameter of 1.29×103±0.33×103 nm, while in the second fraction the average diameter was 469±171 nm. The average diameter of the PP mesh fibers was about 152×103±5.8×103 nm. This kind of system was therefore considered suitable for further cell studies.


Significant improvement of biocompatibility of polypropylene mesh for incisional hernia repair by using poly-ε-caprolactone nanofibers functionalized with thrombocyte-rich solution.

Plencner M, Prosecká E, Rampichová M, East B, Buzgo M, Vysloužilová L, Hoch J, Amler E - Int J Nanomedicine (2015)

Scanning electron microscopy of the scaffolds. (A) Poly-ε-caprolactone (PCL) nanofibers, (B) polypropylene (PP) mesh, and (C) PP mesh functionalized with PCL nanofibers.Notes: (A) Magnification ×230, scale bar 50 μm; (B and C) magnification ×18, scale bar 500 μm.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4388102&req=5

f2-ijn-10-2635: Scanning electron microscopy of the scaffolds. (A) Poly-ε-caprolactone (PCL) nanofibers, (B) polypropylene (PP) mesh, and (C) PP mesh functionalized with PCL nanofibers.Notes: (A) Magnification ×230, scale bar 50 μm; (B and C) magnification ×18, scale bar 500 μm.
Mentions: The functionalized mesh was prepared by attaching the PP mesh onto the collector before the electrospinning process. PCL nanofibers were deposited on the surface of the PP mesh. The PP mesh with nanofibers was exposed in an aqueous environment for 2 weeks without any visual effect on the functionalized mesh. Three types of functionalized scaffolds were examined using SEM in secondary electron mode. Samples of the PP mesh (Figure 2B) were functionalized with PCL nanofibers (Figure 2A) to create a composite scaffold (Figure 2C). SEM revealed randomly oriented nanofibers and their deposition onto the PP mesh. Stereological analyses divided the nanofibers into two fractions of PCL fibers. The first fraction contained fibers with an average diameter of 1.29×103±0.33×103 nm, while in the second fraction the average diameter was 469±171 nm. The average diameter of the PP mesh fibers was about 152×103±5.8×103 nm. This kind of system was therefore considered suitable for further cell studies.

Bottom Line: Nonetheless, the ideal mesh does not exist yet; it still needs to be developed.Compared with polypropylene mesh alone, this composite scaffold provided better adhesion, growth, metabolic activity, proliferation, and viability of mouse fibroblasts in all tests and was even better than a polypropylene mesh functionalized with PCL nanofibers.The gradual release of growth factors from biocompatible nanofiber-modified scaffolds seems to be a promising approach in tissue engineering and regenerative medicine.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biophysics, 2nd Faculty of Medicine, Charles University in Prague, Prague, Czech Republic ; Laboratory of Tissue Engineering, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic.

ABSTRACT
Incisional hernia is the most common postoperative complication, affecting up to 20% of patients after abdominal surgery. Insertion of a synthetic surgical mesh has become the standard of care in ventral hernia repair. However, the implementation of a mesh does not reduce the risk of recurrence and the onset of hernia recurrence is only delayed by 2-3 years. Nowadays, more than 100 surgical meshes are available on the market, with polypropylene the most widely used for ventral hernia repair. Nonetheless, the ideal mesh does not exist yet; it still needs to be developed. Polycaprolactone nanofibers appear to be a suitable material for different kinds of cells, including fibroblasts, chondrocytes, and mesenchymal stem cells. The aim of the study reported here was to develop a functionalized scaffold for ventral hernia regeneration. We prepared a novel composite scaffold based on a polypropylene surgical mesh functionalized with poly-ε-caprolactone (PCL) nanofibers and adhered thrombocytes as a natural source of growth factors. In extensive in vitro tests, we proved the biocompatibility of PCL nanofibers with adhered thrombocytes deposited on a polypropylene mesh. Compared with polypropylene mesh alone, this composite scaffold provided better adhesion, growth, metabolic activity, proliferation, and viability of mouse fibroblasts in all tests and was even better than a polypropylene mesh functionalized with PCL nanofibers. The gradual release of growth factors from biocompatible nanofiber-modified scaffolds seems to be a promising approach in tissue engineering and regenerative medicine.

No MeSH data available.


Related in: MedlinePlus