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Significant improvement of biocompatibility of polypropylene mesh for incisional hernia repair by using poly-ε-caprolactone nanofibers functionalized with thrombocyte-rich solution.

Plencner M, Prosecká E, Rampichová M, East B, Buzgo M, Vysloužilová L, Hoch J, Amler E - Int J Nanomedicine (2015)

Bottom Line: Nonetheless, the ideal mesh does not exist yet; it still needs to be developed.Compared with polypropylene mesh alone, this composite scaffold provided better adhesion, growth, metabolic activity, proliferation, and viability of mouse fibroblasts in all tests and was even better than a polypropylene mesh functionalized with PCL nanofibers.The gradual release of growth factors from biocompatible nanofiber-modified scaffolds seems to be a promising approach in tissue engineering and regenerative medicine.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biophysics, 2nd Faculty of Medicine, Charles University in Prague, Prague, Czech Republic ; Laboratory of Tissue Engineering, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic.

ABSTRACT
Incisional hernia is the most common postoperative complication, affecting up to 20% of patients after abdominal surgery. Insertion of a synthetic surgical mesh has become the standard of care in ventral hernia repair. However, the implementation of a mesh does not reduce the risk of recurrence and the onset of hernia recurrence is only delayed by 2-3 years. Nowadays, more than 100 surgical meshes are available on the market, with polypropylene the most widely used for ventral hernia repair. Nonetheless, the ideal mesh does not exist yet; it still needs to be developed. Polycaprolactone nanofibers appear to be a suitable material for different kinds of cells, including fibroblasts, chondrocytes, and mesenchymal stem cells. The aim of the study reported here was to develop a functionalized scaffold for ventral hernia regeneration. We prepared a novel composite scaffold based on a polypropylene surgical mesh functionalized with poly-ε-caprolactone (PCL) nanofibers and adhered thrombocytes as a natural source of growth factors. In extensive in vitro tests, we proved the biocompatibility of PCL nanofibers with adhered thrombocytes deposited on a polypropylene mesh. Compared with polypropylene mesh alone, this composite scaffold provided better adhesion, growth, metabolic activity, proliferation, and viability of mouse fibroblasts in all tests and was even better than a polypropylene mesh functionalized with PCL nanofibers. The gradual release of growth factors from biocompatible nanofiber-modified scaffolds seems to be a promising approach in tissue engineering and regenerative medicine.

No MeSH data available.


Related in: MedlinePlus

Methodology of the scaffold fabrication. Poly-ε-caprolactone (PCL) nanofibers were prepared by an electrospinning method. Electrospun nanofibers were deposited on a polypropylene (PP) surgical mesh, which was attached to the grounded collecting electrode from each side. PP covered with PCL nanofibers was cut into round patches of 6 mm in diameter, sterilized, and immersed in thrombocytes-rich solution (TRS) for 2 hours. The nonadhered thrombocytes were removed by rinsing twice in phosphate-buffered saline. The composite scaffolds were placed in a new well, seeded with 3T3 fibroblasts and tested in vitro.Notes: (1) Syringe and metering pump, (2) needle serving as the electrode, (3) stable part of the jet, (4) whipping/coiling zone, (5) collector covered with PP, (6) ground, and (7) high-voltage supply.
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f1-ijn-10-2635: Methodology of the scaffold fabrication. Poly-ε-caprolactone (PCL) nanofibers were prepared by an electrospinning method. Electrospun nanofibers were deposited on a polypropylene (PP) surgical mesh, which was attached to the grounded collecting electrode from each side. PP covered with PCL nanofibers was cut into round patches of 6 mm in diameter, sterilized, and immersed in thrombocytes-rich solution (TRS) for 2 hours. The nonadhered thrombocytes were removed by rinsing twice in phosphate-buffered saline. The composite scaffolds were placed in a new well, seeded with 3T3 fibroblasts and tested in vitro.Notes: (1) Syringe and metering pump, (2) needle serving as the electrode, (3) stable part of the jet, (4) whipping/coiling zone, (5) collector covered with PP, (6) ground, and (7) high-voltage supply.

Mentions: PCL nanofibers were prepared by an electrospinning method from PCL with molecular weight (MW) 45,000 (Sigma-Aldrich, St Louis, MO, USA).39 Electrospinning was performed from a 14% solution of PCL dissolved in chloroform:ethanol at a ratio of 8:2. A high-voltage source generated voltages of up to 50 kV, and the polymer solution was connected to a high-voltage source. Electrospun nanofibers were deposited on the grounded collecting electrode. A PP surgical mesh (Prolene, Ethicon Inc, Somerville, NJ, USA) was coated with PCL nanofibers. Prolene was attached to the grounded collector, and PCL nanofibers were deposited on the mesh from each side (Figure 1).


Significant improvement of biocompatibility of polypropylene mesh for incisional hernia repair by using poly-ε-caprolactone nanofibers functionalized with thrombocyte-rich solution.

Plencner M, Prosecká E, Rampichová M, East B, Buzgo M, Vysloužilová L, Hoch J, Amler E - Int J Nanomedicine (2015)

Methodology of the scaffold fabrication. Poly-ε-caprolactone (PCL) nanofibers were prepared by an electrospinning method. Electrospun nanofibers were deposited on a polypropylene (PP) surgical mesh, which was attached to the grounded collecting electrode from each side. PP covered with PCL nanofibers was cut into round patches of 6 mm in diameter, sterilized, and immersed in thrombocytes-rich solution (TRS) for 2 hours. The nonadhered thrombocytes were removed by rinsing twice in phosphate-buffered saline. The composite scaffolds were placed in a new well, seeded with 3T3 fibroblasts and tested in vitro.Notes: (1) Syringe and metering pump, (2) needle serving as the electrode, (3) stable part of the jet, (4) whipping/coiling zone, (5) collector covered with PP, (6) ground, and (7) high-voltage supply.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4388102&req=5

f1-ijn-10-2635: Methodology of the scaffold fabrication. Poly-ε-caprolactone (PCL) nanofibers were prepared by an electrospinning method. Electrospun nanofibers were deposited on a polypropylene (PP) surgical mesh, which was attached to the grounded collecting electrode from each side. PP covered with PCL nanofibers was cut into round patches of 6 mm in diameter, sterilized, and immersed in thrombocytes-rich solution (TRS) for 2 hours. The nonadhered thrombocytes were removed by rinsing twice in phosphate-buffered saline. The composite scaffolds were placed in a new well, seeded with 3T3 fibroblasts and tested in vitro.Notes: (1) Syringe and metering pump, (2) needle serving as the electrode, (3) stable part of the jet, (4) whipping/coiling zone, (5) collector covered with PP, (6) ground, and (7) high-voltage supply.
Mentions: PCL nanofibers were prepared by an electrospinning method from PCL with molecular weight (MW) 45,000 (Sigma-Aldrich, St Louis, MO, USA).39 Electrospinning was performed from a 14% solution of PCL dissolved in chloroform:ethanol at a ratio of 8:2. A high-voltage source generated voltages of up to 50 kV, and the polymer solution was connected to a high-voltage source. Electrospun nanofibers were deposited on the grounded collecting electrode. A PP surgical mesh (Prolene, Ethicon Inc, Somerville, NJ, USA) was coated with PCL nanofibers. Prolene was attached to the grounded collector, and PCL nanofibers were deposited on the mesh from each side (Figure 1).

Bottom Line: Nonetheless, the ideal mesh does not exist yet; it still needs to be developed.Compared with polypropylene mesh alone, this composite scaffold provided better adhesion, growth, metabolic activity, proliferation, and viability of mouse fibroblasts in all tests and was even better than a polypropylene mesh functionalized with PCL nanofibers.The gradual release of growth factors from biocompatible nanofiber-modified scaffolds seems to be a promising approach in tissue engineering and regenerative medicine.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biophysics, 2nd Faculty of Medicine, Charles University in Prague, Prague, Czech Republic ; Laboratory of Tissue Engineering, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic.

ABSTRACT
Incisional hernia is the most common postoperative complication, affecting up to 20% of patients after abdominal surgery. Insertion of a synthetic surgical mesh has become the standard of care in ventral hernia repair. However, the implementation of a mesh does not reduce the risk of recurrence and the onset of hernia recurrence is only delayed by 2-3 years. Nowadays, more than 100 surgical meshes are available on the market, with polypropylene the most widely used for ventral hernia repair. Nonetheless, the ideal mesh does not exist yet; it still needs to be developed. Polycaprolactone nanofibers appear to be a suitable material for different kinds of cells, including fibroblasts, chondrocytes, and mesenchymal stem cells. The aim of the study reported here was to develop a functionalized scaffold for ventral hernia regeneration. We prepared a novel composite scaffold based on a polypropylene surgical mesh functionalized with poly-ε-caprolactone (PCL) nanofibers and adhered thrombocytes as a natural source of growth factors. In extensive in vitro tests, we proved the biocompatibility of PCL nanofibers with adhered thrombocytes deposited on a polypropylene mesh. Compared with polypropylene mesh alone, this composite scaffold provided better adhesion, growth, metabolic activity, proliferation, and viability of mouse fibroblasts in all tests and was even better than a polypropylene mesh functionalized with PCL nanofibers. The gradual release of growth factors from biocompatible nanofiber-modified scaffolds seems to be a promising approach in tissue engineering and regenerative medicine.

No MeSH data available.


Related in: MedlinePlus